Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI; R2A_HEPES media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS14930 and ABIE51_RS14935 are separated by 89 nucleotides ABIE51_RS14935 and ABIE51_RS14940 overlap by 4 nucleotides ABIE51_RS14940 and ABIE51_RS14945 are separated by 36 nucleotides ABIE51_RS14945 and ABIE51_RS14950 are separated by 0 nucleotides
ABIE51_RS14930: ABIE51_RS14930 - ATP-dependent RNA helicase DbpA, at 3,241,089 to 3,242,495
_RS14930
ABIE51_RS14935: ABIE51_RS14935 - hypothetical protein, at 3,242,585 to 3,243,178
_RS14935
ABIE51_RS14940: ABIE51_RS14940 - GIY-YIG nuclease family protein, at 3,243,175 to 3,243,444
_RS14940
ABIE51_RS14945: ABIE51_RS14945 - RNA-binding S4 domain-containing protein, at 3,243,481 to 3,243,705
_RS14945
ABIE51_RS14950: ABIE51_RS14950 - hypothetical protein, at 3,243,706 to 3,244,851
_RS14950
Position (kb)
3243
3244 Strain fitness (log2 ratio)
-2
-1
0
1 at 3242.547 kb on + strand at 3242.548 kb on - strand at 3242.565 kb on + strand at 3242.566 kb on - strand at 3242.723 kb on + strand, within ABIE51_RS14935 at 3242.724 kb on - strand, within ABIE51_RS14935 at 3242.724 kb on - strand, within ABIE51_RS14935 at 3242.829 kb on - strand, within ABIE51_RS14935 at 3242.847 kb on - strand, within ABIE51_RS14935 at 3242.847 kb on - strand, within ABIE51_RS14935 at 3242.847 kb on - strand, within ABIE51_RS14935 at 3242.903 kb on + strand, within ABIE51_RS14935 at 3242.922 kb on - strand, within ABIE51_RS14935 at 3242.969 kb on + strand, within ABIE51_RS14935 at 3243.014 kb on + strand, within ABIE51_RS14935 at 3243.014 kb on + strand, within ABIE51_RS14935 at 3243.014 kb on + strand, within ABIE51_RS14935 at 3243.035 kb on + strand, within ABIE51_RS14935 at 3243.063 kb on + strand, within ABIE51_RS14935 at 3243.064 kb on - strand, within ABIE51_RS14935 at 3243.087 kb on + strand, within ABIE51_RS14935 at 3243.088 kb on - strand, within ABIE51_RS14935 at 3243.088 kb on - strand, within ABIE51_RS14935 at 3243.088 kb on - strand, within ABIE51_RS14935 at 3243.153 kb on - strand at 3243.162 kb on - strand at 3243.168 kb on - strand at 3243.208 kb on + strand, within ABIE51_RS14940 at 3243.214 kb on + strand, within ABIE51_RS14940 at 3243.215 kb on - strand, within ABIE51_RS14940 at 3243.858 kb on - strand, within ABIE51_RS14950 at 3244.118 kb on + strand, within ABIE51_RS14950 at 3244.412 kb on + strand, within ABIE51_RS14950 at 3244.413 kb on - strand, within ABIE51_RS14950 at 3244.652 kb on + strand, within ABIE51_RS14950 at 3244.652 kb on + strand, within ABIE51_RS14950 at 3244.676 kb on - strand, within ABIE51_RS14950
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI; R2A_HEPES media remove 3,242,547 + +1.1 3,242,548 - +0.0 3,242,565 + +0.5 3,242,566 - +0.2 3,242,723 + ABIE51_RS14935 0.23 +0.2 3,242,724 - ABIE51_RS14935 0.23 +0.6 3,242,724 - ABIE51_RS14935 0.23 +0.9 3,242,829 - ABIE51_RS14935 0.41 -0.1 3,242,847 - ABIE51_RS14935 0.44 -0.1 3,242,847 - ABIE51_RS14935 0.44 -0.2 3,242,847 - ABIE51_RS14935 0.44 +0.1 3,242,903 + ABIE51_RS14935 0.54 -0.5 3,242,922 - ABIE51_RS14935 0.57 +0.3 3,242,969 + ABIE51_RS14935 0.65 +0.5 3,243,014 + ABIE51_RS14935 0.72 -0.7 3,243,014 + ABIE51_RS14935 0.72 +0.5 3,243,014 + ABIE51_RS14935 0.72 -0.1 3,243,035 + ABIE51_RS14935 0.76 -0.4 3,243,063 + ABIE51_RS14935 0.80 -0.5 3,243,064 - ABIE51_RS14935 0.81 +0.3 3,243,087 + ABIE51_RS14935 0.85 -0.1 3,243,088 - ABIE51_RS14935 0.85 -1.1 3,243,088 - ABIE51_RS14935 0.85 -0.3 3,243,088 - ABIE51_RS14935 0.85 -0.3 3,243,153 - +1.5 3,243,162 - +1.2 3,243,168 - -0.7 3,243,208 + ABIE51_RS14940 0.12 -0.4 3,243,214 + ABIE51_RS14940 0.14 -2.3 3,243,215 - ABIE51_RS14940 0.15 -0.3 3,243,858 - ABIE51_RS14950 0.13 -0.2 3,244,118 + ABIE51_RS14950 0.36 +0.1 3,244,412 + ABIE51_RS14950 0.62 -0.9 3,244,413 - ABIE51_RS14950 0.62 -0.1 3,244,652 + ABIE51_RS14950 0.83 +0.5 3,244,652 + ABIE51_RS14950 0.83 -0.4 3,244,676 - ABIE51_RS14950 0.85 -0.2
Or see this region's nucleotide sequence