Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI; R2A_HEPES media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS04950 and ABIE51_RS04955 overlap by 4 nucleotides ABIE51_RS04955 and ABIE51_RS04960 overlap by 8 nucleotides ABIE51_RS04960 and ABIE51_RS04965 overlap by 4 nucleotides
ABIE51_RS04950: ABIE51_RS04950 - cobyrinate a,c-diamide synthase, at 1,074,092 to 1,075,390
_RS04950
ABIE51_RS04955: ABIE51_RS04955 - adenosylcobinamide-phosphate synthase CbiB, at 1,075,387 to 1,076,304
_RS04955
ABIE51_RS04960: ABIE51_RS04960 - threonine-phosphate decarboxylase CobD, at 1,076,297 to 1,077,292
_RS04960
ABIE51_RS04965: ABIE51_RS04965 - cobyric acid synthase, at 1,077,289 to 1,078,761
_RS04965
Position (kb) 1076
1077
1078 Strain fitness (log2 ratio) -1
0
1 at 1075.343 kb on + strand at 1075.637 kb on - strand, within ABIE51_RS04955 at 1075.637 kb on - strand, within ABIE51_RS04955 at 1076.008 kb on + strand, within ABIE51_RS04955 at 1076.008 kb on + strand, within ABIE51_RS04955 at 1076.008 kb on + strand, within ABIE51_RS04955 at 1076.009 kb on - strand, within ABIE51_RS04955 at 1076.339 kb on + strand at 1076.340 kb on - strand at 1076.478 kb on - strand, within ABIE51_RS04960 at 1076.478 kb on - strand, within ABIE51_RS04960 at 1076.831 kb on - strand, within ABIE51_RS04960 at 1076.926 kb on - strand, within ABIE51_RS04960 at 1076.926 kb on - strand, within ABIE51_RS04960 at 1077.610 kb on + strand, within ABIE51_RS04965 at 1077.746 kb on - strand, within ABIE51_RS04965 at 1077.803 kb on + strand, within ABIE51_RS04965
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI; R2A_HEPES media remove 1,075,343 + -0.4 1,075,637 - ABIE51_RS04955 0.27 -0.3 1,075,637 - ABIE51_RS04955 0.27 +1.6 1,076,008 + ABIE51_RS04955 0.68 -0.3 1,076,008 + ABIE51_RS04955 0.68 -0.9 1,076,008 + ABIE51_RS04955 0.68 +0.3 1,076,009 - ABIE51_RS04955 0.68 +0.5 1,076,339 + +0.3 1,076,340 - +1.0 1,076,478 - ABIE51_RS04960 0.18 -0.2 1,076,478 - ABIE51_RS04960 0.18 +0.2 1,076,831 - ABIE51_RS04960 0.54 +0.3 1,076,926 - ABIE51_RS04960 0.63 -0.1 1,076,926 - ABIE51_RS04960 0.63 -0.2 1,077,610 + ABIE51_RS04965 0.22 -0.4 1,077,746 - ABIE51_RS04965 0.31 +0.3 1,077,803 + ABIE51_RS04965 0.35 +0.4
Or see this region's nucleotide sequence