Experiment: Parafilmed volatile agar plate with Trichoderma atroviridae IMI; R2A_HEPES media
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS04670 and ABIE51_RS04675 overlap by 4 nucleotides ABIE51_RS04675 and ABIE51_RS04680 are separated by 1 nucleotides ABIE51_RS04680 and ABIE51_RS04685 are separated by 200 nucleotides ABIE51_RS04685 and ABIE51_RS04690 overlap by 17 nucleotides
ABIE51_RS04670: ABIE51_RS04670 - FAD-linked oxidase C-terminal domain-containing protein, at 1,009,726 to 1,011,108
_RS04670
ABIE51_RS04675: ABIE51_RS04675 - metal-dependent hydrolase, at 1,011,105 to 1,011,638
_RS04675
ABIE51_RS04680: ABIE51_RS04680 - DUF4349 domain-containing protein, at 1,011,640 to 1,012,509
_RS04680
ABIE51_RS04685: ABIE51_RS04685 - YraN family protein, at 1,012,710 to 1,013,090
_RS04685
ABIE51_RS04690: ABIE51_RS04690 - penicillin-binding protein activator, at 1,013,074 to 1,014,468
_RS04690
Position (kb)
1011
1012
1013 Strain fitness (log2 ratio)
-1
0
1 at 1010.640 kb on - strand, within ABIE51_RS04670 at 1010.696 kb on + strand, within ABIE51_RS04670 at 1011.020 kb on + strand at 1011.020 kb on + strand at 1011.021 kb on - strand at 1011.021 kb on - strand at 1011.021 kb on - strand at 1011.021 kb on - strand at 1011.121 kb on - strand at 1011.174 kb on + strand, within ABIE51_RS04675 at 1011.396 kb on + strand, within ABIE51_RS04675 at 1011.397 kb on - strand, within ABIE51_RS04675 at 1011.397 kb on - strand, within ABIE51_RS04675 at 1011.487 kb on + strand, within ABIE51_RS04675 at 1011.487 kb on + strand, within ABIE51_RS04675 at 1011.488 kb on - strand, within ABIE51_RS04675 at 1011.545 kb on + strand, within ABIE51_RS04675 at 1011.546 kb on - strand, within ABIE51_RS04675 at 1011.546 kb on - strand, within ABIE51_RS04675 at 1011.546 kb on - strand, within ABIE51_RS04675 at 1011.546 kb on - strand, within ABIE51_RS04675 at 1011.713 kb on + strand at 1011.714 kb on - strand at 1011.743 kb on - strand, within ABIE51_RS04680 at 1011.743 kb on - strand, within ABIE51_RS04680 at 1011.794 kb on - strand, within ABIE51_RS04680 at 1012.188 kb on + strand, within ABIE51_RS04680 at 1012.188 kb on + strand, within ABIE51_RS04680 at 1012.188 kb on + strand, within ABIE51_RS04680 at 1012.189 kb on - strand, within ABIE51_RS04680 at 1012.189 kb on - strand, within ABIE51_RS04680 at 1012.189 kb on - strand, within ABIE51_RS04680 at 1012.189 kb on - strand, within ABIE51_RS04680 at 1012.190 kb on + strand, within ABIE51_RS04680 at 1012.190 kb on + strand, within ABIE51_RS04680 at 1012.190 kb on + strand, within ABIE51_RS04680 at 1012.190 kb on + strand, within ABIE51_RS04680 at 1012.190 kb on + strand, within ABIE51_RS04680 at 1012.191 kb on - strand, within ABIE51_RS04680 at 1012.191 kb on - strand, within ABIE51_RS04680 at 1012.191 kb on - strand, within ABIE51_RS04680 at 1012.191 kb on - strand, within ABIE51_RS04680 at 1012.191 kb on - strand, within ABIE51_RS04680 at 1012.286 kb on + strand, within ABIE51_RS04680 at 1012.287 kb on - strand, within ABIE51_RS04680 at 1012.287 kb on - strand, within ABIE51_RS04680 at 1012.287 kb on - strand, within ABIE51_RS04680 at 1012.326 kb on - strand, within ABIE51_RS04680 at 1012.588 kb on + strand at 1012.711 kb on + strand at 1012.711 kb on + strand at 1012.711 kb on + strand at 1012.712 kb on - strand at 1012.712 kb on - strand at 1012.868 kb on + strand, within ABIE51_RS04685 at 1012.868 kb on + strand, within ABIE51_RS04685 at 1012.868 kb on + strand, within ABIE51_RS04685 at 1012.868 kb on + strand, within ABIE51_RS04685 at 1012.868 kb on + strand, within ABIE51_RS04685 at 1012.869 kb on - strand, within ABIE51_RS04685 at 1012.882 kb on + strand, within ABIE51_RS04685 at 1012.984 kb on + strand, within ABIE51_RS04685 at 1012.985 kb on - strand, within ABIE51_RS04685 at 1012.985 kb on - strand, within ABIE51_RS04685 at 1013.001 kb on + strand, within ABIE51_RS04685 at 1013.001 kb on + strand, within ABIE51_RS04685 at 1013.002 kb on - strand, within ABIE51_RS04685 at 1013.229 kb on - strand, within ABIE51_RS04690
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile agar plate with Trichoderma atroviridae IMI; R2A_HEPES media remove 1,010,640 - ABIE51_RS04670 0.66 +0.9 1,010,696 + ABIE51_RS04670 0.70 +0.9 1,011,020 + +0.9 1,011,020 + -0.7 1,011,021 - +0.0 1,011,021 - -0.1 1,011,021 - -0.5 1,011,021 - -0.1 1,011,121 - +0.1 1,011,174 + ABIE51_RS04675 0.13 +0.2 1,011,396 + ABIE51_RS04675 0.54 +0.3 1,011,397 - ABIE51_RS04675 0.55 -1.7 1,011,397 - ABIE51_RS04675 0.55 +0.2 1,011,487 + ABIE51_RS04675 0.72 -0.0 1,011,487 + ABIE51_RS04675 0.72 +0.1 1,011,488 - ABIE51_RS04675 0.72 -0.3 1,011,545 + ABIE51_RS04675 0.82 -0.1 1,011,546 - ABIE51_RS04675 0.83 -0.3 1,011,546 - ABIE51_RS04675 0.83 +0.3 1,011,546 - ABIE51_RS04675 0.83 -0.2 1,011,546 - ABIE51_RS04675 0.83 +0.2 1,011,713 + +1.4 1,011,714 - +0.0 1,011,743 - ABIE51_RS04680 0.12 -0.5 1,011,743 - ABIE51_RS04680 0.12 +0.0 1,011,794 - ABIE51_RS04680 0.18 +0.4 1,012,188 + ABIE51_RS04680 0.63 +0.5 1,012,188 + ABIE51_RS04680 0.63 -0.6 1,012,188 + ABIE51_RS04680 0.63 +0.9 1,012,189 - ABIE51_RS04680 0.63 +1.1 1,012,189 - ABIE51_RS04680 0.63 -0.5 1,012,189 - ABIE51_RS04680 0.63 +0.1 1,012,189 - ABIE51_RS04680 0.63 +0.8 1,012,190 + ABIE51_RS04680 0.63 +0.7 1,012,190 + ABIE51_RS04680 0.63 -1.0 1,012,190 + ABIE51_RS04680 0.63 -0.6 1,012,190 + ABIE51_RS04680 0.63 -0.0 1,012,190 + ABIE51_RS04680 0.63 +0.9 1,012,191 - ABIE51_RS04680 0.63 +0.9 1,012,191 - ABIE51_RS04680 0.63 -0.1 1,012,191 - ABIE51_RS04680 0.63 -0.1 1,012,191 - ABIE51_RS04680 0.63 -0.1 1,012,191 - ABIE51_RS04680 0.63 +0.9 1,012,286 + ABIE51_RS04680 0.74 -0.3 1,012,287 - ABIE51_RS04680 0.74 +0.2 1,012,287 - ABIE51_RS04680 0.74 -0.0 1,012,287 - ABIE51_RS04680 0.74 -0.1 1,012,326 - ABIE51_RS04680 0.79 -0.4 1,012,588 + -0.3 1,012,711 + +0.1 1,012,711 + -0.0 1,012,711 + +0.2 1,012,712 - +0.3 1,012,712 - +0.2 1,012,868 + ABIE51_RS04685 0.41 +0.9 1,012,868 + ABIE51_RS04685 0.41 -0.9 1,012,868 + ABIE51_RS04685 0.41 +0.3 1,012,868 + ABIE51_RS04685 0.41 -0.4 1,012,868 + ABIE51_RS04685 0.41 -0.6 1,012,869 - ABIE51_RS04685 0.42 -0.3 1,012,882 + ABIE51_RS04685 0.45 +0.1 1,012,984 + ABIE51_RS04685 0.72 -0.6 1,012,985 - ABIE51_RS04685 0.72 -0.5 1,012,985 - ABIE51_RS04685 0.72 -0.1 1,013,001 + ABIE51_RS04685 0.76 -0.0 1,013,001 + ABIE51_RS04685 0.76 +0.3 1,013,002 - ABIE51_RS04685 0.77 -0.4 1,013,229 - ABIE51_RS04690 0.11 -0.2
Or see this region's nucleotide sequence