Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS21660

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS21650 and ECOLIN_RS21655 overlap by 4 nucleotidesECOLIN_RS21655 and ECOLIN_RS21660 are separated by 19 nucleotidesECOLIN_RS21660 and ECOLIN_RS21665 are separated by 64 nucleotides ECOLIN_RS21650: ECOLIN_RS21650 - acetolactate synthase 2 catalytic subunit, at 4,348,228 to 4,349,874 _RS21650 ECOLIN_RS21655: ECOLIN_RS21655 - acetolactate synthase 2 small subunit, at 4,349,871 to 4,350,134 _RS21655 ECOLIN_RS21660: ECOLIN_RS21660 - branched-chain-amino-acid transaminase, at 4,350,154 to 4,351,083 _RS21660 ECOLIN_RS21665: ECOLIN_RS21665 - dihydroxy-acid dehydratase, at 4,351,148 to 4,352,998 _RS21665 Position (kb) 4350 4351 4352Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 4349.157 kb on - strand, within ECOLIN_RS21650at 4349.169 kb on - strand, within ECOLIN_RS21650at 4349.189 kb on + strand, within ECOLIN_RS21650at 4349.190 kb on - strand, within ECOLIN_RS21650at 4349.190 kb on - strand, within ECOLIN_RS21650at 4349.190 kb on - strand, within ECOLIN_RS21650at 4349.201 kb on + strand, within ECOLIN_RS21650at 4349.201 kb on + strand, within ECOLIN_RS21650at 4349.280 kb on + strand, within ECOLIN_RS21650at 4349.500 kb on - strand, within ECOLIN_RS21650at 4349.582 kb on + strand, within ECOLIN_RS21650at 4349.845 kb on - strandat 4349.868 kb on - strandat 4349.933 kb on - strand, within ECOLIN_RS21655at 4349.976 kb on - strand, within ECOLIN_RS21655at 4350.005 kb on - strand, within ECOLIN_RS21655at 4350.005 kb on - strand, within ECOLIN_RS21655at 4350.005 kb on - strand, within ECOLIN_RS21655at 4350.046 kb on + strand, within ECOLIN_RS21655at 4350.046 kb on + strand, within ECOLIN_RS21655at 4350.047 kb on - strand, within ECOLIN_RS21655at 4350.062 kb on - strand, within ECOLIN_RS21655at 4350.149 kb on + strandat 4350.175 kb on + strandat 4350.175 kb on + strandat 4350.175 kb on + strandat 4350.305 kb on + strand, within ECOLIN_RS21660at 4350.306 kb on - strand, within ECOLIN_RS21660at 4350.324 kb on + strand, within ECOLIN_RS21660at 4350.325 kb on - strand, within ECOLIN_RS21660at 4350.439 kb on + strand, within ECOLIN_RS21660at 4350.439 kb on + strand, within ECOLIN_RS21660at 4350.541 kb on + strand, within ECOLIN_RS21660at 4350.542 kb on - strand, within ECOLIN_RS21660at 4350.642 kb on + strand, within ECOLIN_RS21660at 4350.642 kb on + strand, within ECOLIN_RS21660at 4350.646 kb on + strand, within ECOLIN_RS21660at 4350.647 kb on - strand, within ECOLIN_RS21660at 4350.724 kb on + strand, within ECOLIN_RS21660at 4350.724 kb on + strand, within ECOLIN_RS21660at 4350.724 kb on + strand, within ECOLIN_RS21660at 4350.916 kb on - strand, within ECOLIN_RS21660at 4350.956 kb on + strand, within ECOLIN_RS21660at 4351.064 kb on + strandat 4351.064 kb on + strandat 4351.074 kb on + strandat 4351.085 kb on + strandat 4351.142 kb on + strandat 4351.142 kb on + strandat 4351.142 kb on + strandat 4351.142 kb on + strandat 4351.142 kb on + strandat 4351.143 kb on - strandat 4351.147 kb on + strandat 4351.147 kb on + strandat 4351.148 kb on - strandat 4351.153 kb on + strandat 4351.153 kb on + strandat 4351.153 kb on + strandat 4351.189 kb on + strandat 4351.190 kb on - strandat 4351.421 kb on + strand, within ECOLIN_RS21665at 4351.421 kb on + strand, within ECOLIN_RS21665at 4351.463 kb on + strand, within ECOLIN_RS21665at 4351.463 kb on + strand, within ECOLIN_RS21665at 4351.463 kb on + strand, within ECOLIN_RS21665at 4351.463 kb on + strand, within ECOLIN_RS21665at 4351.464 kb on - strand, within ECOLIN_RS21665at 4351.464 kb on - strand, within ECOLIN_RS21665at 4351.464 kb on - strand, within ECOLIN_RS21665at 4351.464 kb on - strand, within ECOLIN_RS21665at 4351.464 kb on - strand, within ECOLIN_RS21665at 4351.464 kb on - strand, within ECOLIN_RS21665at 4351.465 kb on + strand, within ECOLIN_RS21665at 4351.466 kb on - strand, within ECOLIN_RS21665at 4351.883 kb on + strand, within ECOLIN_RS21665at 4352.001 kb on + strand, within ECOLIN_RS21665at 4352.070 kb on + strand, within ECOLIN_RS21665

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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4,349,157 - ECOLIN_RS21650 0.56 -0.9
4,349,169 - ECOLIN_RS21650 0.57 -0.2
4,349,189 + ECOLIN_RS21650 0.58 -0.9
4,349,190 - ECOLIN_RS21650 0.58 -1.0
4,349,190 - ECOLIN_RS21650 0.58 -0.4
4,349,190 - ECOLIN_RS21650 0.58 -0.2
4,349,201 + ECOLIN_RS21650 0.59 -2.2
4,349,201 + ECOLIN_RS21650 0.59 -0.9
4,349,280 + ECOLIN_RS21650 0.64 -3.1
4,349,500 - ECOLIN_RS21650 0.77 -0.4
4,349,582 + ECOLIN_RS21650 0.82 -1.1
4,349,845 - -0.9
4,349,868 - -1.1
4,349,933 - ECOLIN_RS21655 0.23 -1.4
4,349,976 - ECOLIN_RS21655 0.40 +0.0
4,350,005 - ECOLIN_RS21655 0.51 -0.7
4,350,005 - ECOLIN_RS21655 0.51 -1.1
4,350,005 - ECOLIN_RS21655 0.51 -0.9
4,350,046 + ECOLIN_RS21655 0.66 +2.8
4,350,046 + ECOLIN_RS21655 0.66 -0.3
4,350,047 - ECOLIN_RS21655 0.67 -1.6
4,350,062 - ECOLIN_RS21655 0.72 -1.1
4,350,149 + -2.4
4,350,175 + -0.9
4,350,175 + -1.1
4,350,175 + -1.3
4,350,305 + ECOLIN_RS21660 0.16 -2.5
4,350,306 - ECOLIN_RS21660 0.16 -1.4
4,350,324 + ECOLIN_RS21660 0.18 -1.2
4,350,325 - ECOLIN_RS21660 0.18 -0.7
4,350,439 + ECOLIN_RS21660 0.31 -0.9
4,350,439 + ECOLIN_RS21660 0.31 -1.5
4,350,541 + ECOLIN_RS21660 0.42 -2.1
4,350,542 - ECOLIN_RS21660 0.42 -1.5
4,350,642 + ECOLIN_RS21660 0.52 -1.7
4,350,642 + ECOLIN_RS21660 0.52 -1.5
4,350,646 + ECOLIN_RS21660 0.53 -1.3
4,350,647 - ECOLIN_RS21660 0.53 -3.2
4,350,724 + ECOLIN_RS21660 0.61 -1.4
4,350,724 + ECOLIN_RS21660 0.61 -1.0
4,350,724 + ECOLIN_RS21660 0.61 -1.4
4,350,916 - ECOLIN_RS21660 0.82 -1.3
4,350,956 + ECOLIN_RS21660 0.86 -2.9
4,351,064 + -1.8
4,351,064 + -0.7
4,351,074 + +0.4
4,351,085 + +0.1
4,351,142 + -1.0
4,351,142 + -0.1
4,351,142 + -0.6
4,351,142 + -1.3
4,351,142 + -0.7
4,351,143 - -0.8
4,351,147 + -2.1
4,351,147 + -1.7
4,351,148 - -0.9
4,351,153 + -0.2
4,351,153 + -0.8
4,351,153 + -1.3
4,351,189 + -0.8
4,351,190 - -1.2
4,351,421 + ECOLIN_RS21665 0.15 -1.5
4,351,421 + ECOLIN_RS21665 0.15 -2.2
4,351,463 + ECOLIN_RS21665 0.17 -0.7
4,351,463 + ECOLIN_RS21665 0.17 -0.7
4,351,463 + ECOLIN_RS21665 0.17 -1.4
4,351,463 + ECOLIN_RS21665 0.17 -1.0
4,351,464 - ECOLIN_RS21665 0.17 -2.9
4,351,464 - ECOLIN_RS21665 0.17 -2.2
4,351,464 - ECOLIN_RS21665 0.17 -2.3
4,351,464 - ECOLIN_RS21665 0.17 -0.5
4,351,464 - ECOLIN_RS21665 0.17 -1.4
4,351,464 - ECOLIN_RS21665 0.17 -2.3
4,351,465 + ECOLIN_RS21665 0.17 -0.9
4,351,466 - ECOLIN_RS21665 0.17 -0.4
4,351,883 + ECOLIN_RS21665 0.40 -3.1
4,352,001 + ECOLIN_RS21665 0.46 -2.0
4,352,070 + ECOLIN_RS21665 0.50 -2.2

Or see this region's nucleotide sequence