Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS18035

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS18025 and ECOLIN_RS18030 are separated by 22 nucleotidesECOLIN_RS18030 and ECOLIN_RS18035 are separated by 10 nucleotidesECOLIN_RS18035 and ECOLIN_RS18040 overlap by 11 nucleotidesECOLIN_RS18040 and ECOLIN_RS18045 are separated by 17 nucleotides ECOLIN_RS18025: ECOLIN_RS18025 - tagatose-bisphosphate aldolase subunit KbaZ, at 3,635,273 to 3,636,553 _RS18025 ECOLIN_RS18030: ECOLIN_RS18030 - PTS N-acetylgalactosamine transporter subunit IIB, at 3,636,576 to 3,637,049 _RS18030 ECOLIN_RS18035: ECOLIN_RS18035 - PTS N-acetylgalactosamine transporter subunit IIC, at 3,637,060 to 3,637,839 _RS18035 ECOLIN_RS18040: ECOLIN_RS18040 - PTS N-acetylgalactosamine transporter subunit IID, at 3,637,829 to 3,638,707 _RS18040 ECOLIN_RS18045: ECOLIN_RS18045 - PTS galactosamine/N-acetylgalactosamine transporter subunit IIA, at 3,638,725 to 3,639,159 _RS18045 Position (kb) 3637 3638Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3636.276 kb on - strand, within ECOLIN_RS18025at 3636.317 kb on - strand, within ECOLIN_RS18025at 3636.317 kb on - strand, within ECOLIN_RS18025at 3636.339 kb on - strand, within ECOLIN_RS18025at 3636.447 kb on - strandat 3637.207 kb on - strand, within ECOLIN_RS18035at 3637.318 kb on - strand, within ECOLIN_RS18035at 3637.389 kb on + strand, within ECOLIN_RS18035at 3637.389 kb on + strand, within ECOLIN_RS18035at 3637.389 kb on + strand, within ECOLIN_RS18035at 3637.510 kb on + strand, within ECOLIN_RS18035at 3637.533 kb on + strand, within ECOLIN_RS18035at 3637.672 kb on + strand, within ECOLIN_RS18035at 3637.907 kb on + strandat 3637.908 kb on - strandat 3637.908 kb on - strandat 3637.948 kb on + strand, within ECOLIN_RS18040at 3638.005 kb on + strand, within ECOLIN_RS18040at 3638.065 kb on + strand, within ECOLIN_RS18040at 3638.149 kb on + strand, within ECOLIN_RS18040at 3638.171 kb on - strand, within ECOLIN_RS18040at 3638.259 kb on + strand, within ECOLIN_RS18040at 3638.298 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.455 kb on + strand, within ECOLIN_RS18040at 3638.456 kb on - strand, within ECOLIN_RS18040at 3638.495 kb on + strand, within ECOLIN_RS18040at 3638.495 kb on + strand, within ECOLIN_RS18040at 3638.495 kb on + strand, within ECOLIN_RS18040at 3638.550 kb on + strand, within ECOLIN_RS18040at 3638.601 kb on - strand, within ECOLIN_RS18040at 3638.720 kb on - strandat 3638.722 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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3,636,276 - ECOLIN_RS18025 0.78 -0.6
3,636,317 - ECOLIN_RS18025 0.81 -0.5
3,636,317 - ECOLIN_RS18025 0.81 +0.1
3,636,339 - ECOLIN_RS18025 0.83 +0.2
3,636,447 - -1.4
3,637,207 - ECOLIN_RS18035 0.19 +1.6
3,637,318 - ECOLIN_RS18035 0.33 -0.1
3,637,389 + ECOLIN_RS18035 0.42 +2.0
3,637,389 + ECOLIN_RS18035 0.42 -0.7
3,637,389 + ECOLIN_RS18035 0.42 +0.9
3,637,510 + ECOLIN_RS18035 0.58 -1.3
3,637,533 + ECOLIN_RS18035 0.61 +0.4
3,637,672 + ECOLIN_RS18035 0.78 +0.5
3,637,907 + +0.7
3,637,908 - +0.8
3,637,908 - +0.8
3,637,948 + ECOLIN_RS18040 0.14 -2.2
3,638,005 + ECOLIN_RS18040 0.20 +1.2
3,638,065 + ECOLIN_RS18040 0.27 -0.6
3,638,149 + ECOLIN_RS18040 0.36 -0.3
3,638,171 - ECOLIN_RS18040 0.39 -0.4
3,638,259 + ECOLIN_RS18040 0.49 +0.2
3,638,298 + ECOLIN_RS18040 0.53 -0.1
3,638,455 + ECOLIN_RS18040 0.71 -0.3
3,638,455 + ECOLIN_RS18040 0.71 -0.2
3,638,455 + ECOLIN_RS18040 0.71 -2.2
3,638,455 + ECOLIN_RS18040 0.71 +1.3
3,638,456 - ECOLIN_RS18040 0.71 +1.3
3,638,495 + ECOLIN_RS18040 0.76 -1.1
3,638,495 + ECOLIN_RS18040 0.76 +1.2
3,638,495 + ECOLIN_RS18040 0.76 -0.5
3,638,550 + ECOLIN_RS18040 0.82 -0.3
3,638,601 - ECOLIN_RS18040 0.88 +0.0
3,638,720 - +0.0
3,638,722 + +2.8

Or see this region's nucleotide sequence