Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17140

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17135 and ECOLIN_RS17140 overlap by 1 nucleotidesECOLIN_RS17140 and ECOLIN_RS17145 are separated by 250 nucleotides ECOLIN_RS17135: ECOLIN_RS17135 - glycolate oxidase subunit GlcE, at 3,453,875 to 3,454,927 _RS17135 ECOLIN_RS17140: ECOLIN_RS17140 - glycolate oxidase subunit GlcD, at 3,454,927 to 3,456,426 _RS17140 ECOLIN_RS17145: ECOLIN_RS17145 - transcriptional regulator GlcC, at 3,456,677 to 3,457,441 _RS17145 Position (kb) 3454 3455 3456 3457Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 3453.956 kb on + strandat 3454.305 kb on + strand, within ECOLIN_RS17135at 3454.305 kb on + strand, within ECOLIN_RS17135at 3454.310 kb on + strand, within ECOLIN_RS17135at 3454.310 kb on + strand, within ECOLIN_RS17135at 3454.311 kb on - strand, within ECOLIN_RS17135at 3454.639 kb on + strand, within ECOLIN_RS17135at 3454.640 kb on - strand, within ECOLIN_RS17135at 3454.640 kb on - strand, within ECOLIN_RS17135at 3454.762 kb on - strand, within ECOLIN_RS17135at 3454.766 kb on - strand, within ECOLIN_RS17135at 3454.922 kb on + strandat 3454.955 kb on - strandat 3455.290 kb on + strand, within ECOLIN_RS17140at 3455.291 kb on - strand, within ECOLIN_RS17140at 3455.291 kb on - strand, within ECOLIN_RS17140at 3455.367 kb on + strand, within ECOLIN_RS17140at 3455.368 kb on - strand, within ECOLIN_RS17140at 3455.590 kb on + strand, within ECOLIN_RS17140at 3455.590 kb on + strand, within ECOLIN_RS17140at 3455.591 kb on - strand, within ECOLIN_RS17140at 3455.591 kb on - strand, within ECOLIN_RS17140at 3455.782 kb on - strand, within ECOLIN_RS17140at 3455.782 kb on - strand, within ECOLIN_RS17140at 3455.896 kb on - strand, within ECOLIN_RS17140at 3456.000 kb on - strand, within ECOLIN_RS17140at 3456.413 kb on + strandat 3456.414 kb on - strandat 3456.491 kb on + strandat 3456.492 kb on - strandat 3456.525 kb on + strandat 3456.609 kb on + strandat 3456.634 kb on + strandat 3456.634 kb on + strandat 3456.732 kb on + strandat 3457.231 kb on - strand, within ECOLIN_RS17145at 3457.292 kb on + strand, within ECOLIN_RS17145

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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3,453,956 + -0.3
3,454,305 + ECOLIN_RS17135 0.41 +0.0
3,454,305 + ECOLIN_RS17135 0.41 +0.7
3,454,310 + ECOLIN_RS17135 0.41 -0.4
3,454,310 + ECOLIN_RS17135 0.41 -0.2
3,454,311 - ECOLIN_RS17135 0.41 +0.3
3,454,639 + ECOLIN_RS17135 0.73 -0.2
3,454,640 - ECOLIN_RS17135 0.73 +0.7
3,454,640 - ECOLIN_RS17135 0.73 +0.0
3,454,762 - ECOLIN_RS17135 0.84 -1.0
3,454,766 - ECOLIN_RS17135 0.85 +0.7
3,454,922 + -0.4
3,454,955 - +0.6
3,455,290 + ECOLIN_RS17140 0.24 +0.7
3,455,291 - ECOLIN_RS17140 0.24 -0.8
3,455,291 - ECOLIN_RS17140 0.24 -0.4
3,455,367 + ECOLIN_RS17140 0.29 -0.1
3,455,368 - ECOLIN_RS17140 0.29 -0.7
3,455,590 + ECOLIN_RS17140 0.44 +0.2
3,455,590 + ECOLIN_RS17140 0.44 +1.9
3,455,591 - ECOLIN_RS17140 0.44 -0.2
3,455,591 - ECOLIN_RS17140 0.44 +0.7
3,455,782 - ECOLIN_RS17140 0.57 -0.1
3,455,782 - ECOLIN_RS17140 0.57 +2.0
3,455,896 - ECOLIN_RS17140 0.65 -2.0
3,456,000 - ECOLIN_RS17140 0.72 +0.3
3,456,413 + +0.6
3,456,414 - -0.5
3,456,491 + -0.9
3,456,492 - +0.7
3,456,525 + -0.1
3,456,609 + -0.0
3,456,634 + +0.5
3,456,634 + -4.5
3,456,732 + +0.4
3,457,231 - ECOLIN_RS17145 0.72 -0.2
3,457,292 + ECOLIN_RS17145 0.80 -2.4

Or see this region's nucleotide sequence