Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS12855

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS12840 and ECOLIN_RS12845 overlap by 4 nucleotidesECOLIN_RS12845 and ECOLIN_RS12850 overlap by 4 nucleotidesECOLIN_RS12850 and ECOLIN_RS12855 are separated by 41 nucleotidesECOLIN_RS12855 and ECOLIN_RS12860 overlap by 4 nucleotidesECOLIN_RS12860 and ECOLIN_RS12865 are separated by 12 nucleotides ECOLIN_RS12840: ECOLIN_RS12840 - cytochrome c maturation protein CcmE, at 2,554,464 to 2,554,943 _RS12840 ECOLIN_RS12845: ECOLIN_RS12845 - heme exporter protein CcmD, at 2,554,940 to 2,555,149 _RS12845 ECOLIN_RS12850: ECOLIN_RS12850 - heme exporter protein CcmC, at 2,555,146 to 2,555,883 _RS12850 ECOLIN_RS12855: ECOLIN_RS12855 - heme exporter protein CcmB, at 2,555,925 to 2,556,587 _RS12855 ECOLIN_RS12860: ECOLIN_RS12860 - cytochrome c biogenesis heme-transporting ATPase CcmA, at 2,556,584 to 2,557,207 _RS12860 ECOLIN_RS12865: ECOLIN_RS12865 - cytochrome c-type protein NapC, at 2,557,220 to 2,557,822 _RS12865 Position (kb) 2555 2556 2557Strain fitness (log2 ratio) -1 0 1at 2555.469 kb on - strand, within ECOLIN_RS12850at 2555.971 kb on - strandat 2556.126 kb on - strand, within ECOLIN_RS12855at 2557.357 kb on + strand, within ECOLIN_RS12865at 2557.357 kb on + strand, within ECOLIN_RS12865at 2557.360 kb on - strand, within ECOLIN_RS12865at 2557.502 kb on - strand, within ECOLIN_RS12865

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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2,555,469 - ECOLIN_RS12850 0.44 -1.4
2,555,971 - -0.6
2,556,126 - ECOLIN_RS12855 0.30 +0.1
2,557,357 + ECOLIN_RS12865 0.23 +0.4
2,557,357 + ECOLIN_RS12865 0.23 -0.0
2,557,360 - ECOLIN_RS12865 0.23 -0.1
2,557,502 - ECOLIN_RS12865 0.47 +0.7

Or see this region's nucleotide sequence