Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04575

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04565 and ECOLIN_RS04570 are separated by 59 nucleotidesECOLIN_RS04570 and ECOLIN_RS04575 are separated by 40 nucleotidesECOLIN_RS04575 and ECOLIN_RS04580 are separated by 28 nucleotidesECOLIN_RS04580 and ECOLIN_RS04585 are separated by 225 nucleotides ECOLIN_RS04565: ECOLIN_RS04565 - putrescine ABC transporter permease PotI, at 924,124 to 924,969 _RS04565 ECOLIN_RS04570: ECOLIN_RS04570 - YbjO family protein, at 925,029 to 925,517 _RS04570 ECOLIN_RS04575: ECOLIN_RS04575 - 23S rRNA (uracil(747)-C(5))-methyltransferase RlmC, at 925,558 to 926,685 _RS04575 ECOLIN_RS04580: ECOLIN_RS04580 - arginine ABC transporter substrate-binding protein ArtJ, at 926,714 to 927,445 _RS04580 ECOLIN_RS04585: ECOLIN_RS04585 - arginine ABC transporter permease ArtM, at 927,671 to 928,339 _RS04585 Position (kb) 925 926 927Strain fitness (log2 ratio) -1 0 1at 924.999 kb on + strandat 925.522 kb on + strandat 925.894 kb on + strand, within ECOLIN_RS04575at 926.151 kb on + strand, within ECOLIN_RS04575at 926.753 kb on - strandat 927.093 kb on + strand, within ECOLIN_RS04580at 927.163 kb on + strand, within ECOLIN_RS04580

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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924,999 + -1.0
925,522 + +0.4
925,894 + ECOLIN_RS04575 0.30 +0.6
926,151 + ECOLIN_RS04575 0.53 -0.3
926,753 - -0.3
927,093 + ECOLIN_RS04580 0.52 -0.1
927,163 + ECOLIN_RS04580 0.61 -1.4

Or see this region's nucleotide sequence