Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS02005

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS02000 and ECOLIN_RS02005 are separated by 10 nucleotidesECOLIN_RS02005 and ECOLIN_RS02010 overlap by 8 nucleotidesECOLIN_RS02010 and ECOLIN_RS02020 are separated by 242 nucleotides ECOLIN_RS02000: ECOLIN_RS02000 - (Fe-S)-binding protein, at 404,577 to 405,296 _RS02000 ECOLIN_RS02005: ECOLIN_RS02005 - LutB/LldF family L-lactate oxidation iron-sulfur protein, at 405,307 to 406,734 _RS02005 ECOLIN_RS02010: ECOLIN_RS02010 - lactate utilization protein C, at 406,727 to 407,422 _RS02010 ECOLIN_RS02020: ECOLIN_RS02020 - protein YkgH, at 407,665 to 408,333 _RS02020 Position (kb) 405 406 407Strain fitness (log2 ratio) -3 -2 -1 0 1at 404.506 kb on - strandat 404.844 kb on + strand, within ECOLIN_RS02000at 404.844 kb on + strand, within ECOLIN_RS02000at 404.897 kb on + strand, within ECOLIN_RS02000at 404.927 kb on + strand, within ECOLIN_RS02000at 404.947 kb on + strand, within ECOLIN_RS02000at 404.947 kb on + strand, within ECOLIN_RS02000at 404.947 kb on + strand, within ECOLIN_RS02000at 405.029 kb on + strand, within ECOLIN_RS02000at 405.029 kb on + strand, within ECOLIN_RS02000at 405.190 kb on + strand, within ECOLIN_RS02000at 405.303 kb on - strandat 405.908 kb on + strand, within ECOLIN_RS02005at 406.003 kb on - strand, within ECOLIN_RS02005at 406.011 kb on + strand, within ECOLIN_RS02005at 406.242 kb on + strand, within ECOLIN_RS02005at 406.823 kb on + strand, within ECOLIN_RS02010at 407.121 kb on + strand, within ECOLIN_RS02010at 407.314 kb on + strand, within ECOLIN_RS02010at 407.430 kb on - strandat 407.497 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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404,506 - +0.8
404,844 + ECOLIN_RS02000 0.37 -1.8
404,844 + ECOLIN_RS02000 0.37 +0.5
404,897 + ECOLIN_RS02000 0.44 +0.6
404,927 + ECOLIN_RS02000 0.49 -2.4
404,947 + ECOLIN_RS02000 0.51 +0.3
404,947 + ECOLIN_RS02000 0.51 -1.6
404,947 + ECOLIN_RS02000 0.51 -0.7
405,029 + ECOLIN_RS02000 0.63 +1.6
405,029 + ECOLIN_RS02000 0.63 -0.4
405,190 + ECOLIN_RS02000 0.85 -0.3
405,303 - -0.4
405,908 + ECOLIN_RS02005 0.42 -0.4
406,003 - ECOLIN_RS02005 0.49 -1.3
406,011 + ECOLIN_RS02005 0.49 -2.8
406,242 + ECOLIN_RS02005 0.65 -0.1
406,823 + ECOLIN_RS02010 0.14 +0.8
407,121 + ECOLIN_RS02010 0.57 -0.0
407,314 + ECOLIN_RS02010 0.84 -0.2
407,430 - -0.2
407,497 + -0.6

Or see this region's nucleotide sequence