Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00360

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00355 and ECOLIN_RS00360 are separated by 113 nucleotidesECOLIN_RS00360 and ECOLIN_RS00365 overlap by 17 nucleotides ECOLIN_RS00355: ECOLIN_RS00355 - DedA family protein, at 74,331 to 75,095 _RS00355 ECOLIN_RS00360: ECOLIN_RS00360 - thiamine ABC transporter ATP-binding protein ThiQ, at 75,209 to 75,907 _RS00360 ECOLIN_RS00365: ECOLIN_RS00365 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 75,891 to 77,501 _RS00365 Position (kb) 75 76Strain fitness (log2 ratio) -2 -1 0 1 2 3at 74.282 kb on + strandat 74.354 kb on + strandat 74.366 kb on + strandat 74.366 kb on + strandat 74.373 kb on + strandat 74.373 kb on + strandat 74.491 kb on + strand, within ECOLIN_RS00355at 74.491 kb on + strand, within ECOLIN_RS00355at 74.962 kb on - strand, within ECOLIN_RS00355at 75.060 kb on + strandat 75.060 kb on + strandat 75.169 kb on - strandat 75.208 kb on - strandat 75.211 kb on - strandat 75.211 kb on - strandat 75.603 kb on - strand, within ECOLIN_RS00360at 75.890 kb on - strandat 76.152 kb on + strand, within ECOLIN_RS00365at 76.153 kb on - strand, within ECOLIN_RS00365at 76.474 kb on - strand, within ECOLIN_RS00365at 76.643 kb on + strand, within ECOLIN_RS00365at 76.644 kb on - strand, within ECOLIN_RS00365at 76.810 kb on + strand, within ECOLIN_RS00365at 76.896 kb on + strand, within ECOLIN_RS00365at 76.896 kb on + strand, within ECOLIN_RS00365

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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74,282 + +1.4
74,354 + -0.8
74,366 + +0.1
74,366 + -0.7
74,373 + -1.8
74,373 + -0.2
74,491 + ECOLIN_RS00355 0.21 -0.2
74,491 + ECOLIN_RS00355 0.21 +0.4
74,962 - ECOLIN_RS00355 0.82 +0.3
75,060 + -0.2
75,060 + -0.2
75,169 - -0.4
75,208 - +0.5
75,211 - -0.7
75,211 - -0.5
75,603 - ECOLIN_RS00360 0.56 -0.4
75,890 - +0.4
76,152 + ECOLIN_RS00365 0.16 +0.3
76,153 - ECOLIN_RS00365 0.16 -0.1
76,474 - ECOLIN_RS00365 0.36 -2.0
76,643 + ECOLIN_RS00365 0.47 -1.3
76,644 - ECOLIN_RS00365 0.47 +0.4
76,810 + ECOLIN_RS00365 0.57 +0.3
76,896 + ECOLIN_RS00365 0.62 +3.0
76,896 + ECOLIN_RS00365 0.62 -1.0

Or see this region's nucleotide sequence