Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00215

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00210 and ECOLIN_RS00215 are separated by 127 nucleotidesECOLIN_RS00215 and ECOLIN_RS00220 are separated by 30 nucleotides ECOLIN_RS00210: ECOLIN_RS00210 - L-carnitine CoA-transferase, at 39,887 to 41,104 _RS00210 ECOLIN_RS00215: ECOLIN_RS00215 - crotonobetainyl-CoA dehydrogenase, at 41,232 to 42,374 _RS00215 ECOLIN_RS00220: ECOLIN_RS00220 - L-carnitine/gamma-butyrobetaine antiporter, at 42,405 to 43,919 _RS00220 Position (kb) 41 42 43Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 40.264 kb on + strand, within ECOLIN_RS00210at 40.265 kb on - strand, within ECOLIN_RS00210at 40.265 kb on - strand, within ECOLIN_RS00210at 40.265 kb on - strand, within ECOLIN_RS00210at 40.319 kb on - strand, within ECOLIN_RS00210at 40.387 kb on + strand, within ECOLIN_RS00210at 40.388 kb on - strand, within ECOLIN_RS00210at 40.389 kb on + strand, within ECOLIN_RS00210at 40.408 kb on - strand, within ECOLIN_RS00210at 40.504 kb on - strand, within ECOLIN_RS00210at 40.684 kb on + strand, within ECOLIN_RS00210at 40.686 kb on + strand, within ECOLIN_RS00210at 40.704 kb on + strand, within ECOLIN_RS00210at 40.705 kb on - strand, within ECOLIN_RS00210at 40.933 kb on - strand, within ECOLIN_RS00210at 41.094 kb on - strandat 41.108 kb on + strandat 41.108 kb on + strandat 41.108 kb on + strandat 41.109 kb on - strandat 41.109 kb on - strandat 41.124 kb on + strandat 41.153 kb on + strandat 41.816 kb on + strand, within ECOLIN_RS00215at 41.885 kb on - strand, within ECOLIN_RS00215at 41.919 kb on - strand, within ECOLIN_RS00215at 41.932 kb on + strand, within ECOLIN_RS00215at 42.093 kb on - strand, within ECOLIN_RS00215at 42.122 kb on - strand, within ECOLIN_RS00215at 42.637 kb on + strand, within ECOLIN_RS00220at 42.637 kb on + strand, within ECOLIN_RS00220at 42.638 kb on - strand, within ECOLIN_RS00220at 42.638 kb on - strand, within ECOLIN_RS00220at 42.638 kb on - strand, within ECOLIN_RS00220at 42.795 kb on - strand, within ECOLIN_RS00220at 42.940 kb on + strand, within ECOLIN_RS00220at 42.962 kb on - strand, within ECOLIN_RS00220at 43.011 kb on - strand, within ECOLIN_RS00220at 43.012 kb on + strand, within ECOLIN_RS00220at 43.033 kb on + strand, within ECOLIN_RS00220at 43.134 kb on - strand, within ECOLIN_RS00220at 43.265 kb on + strand, within ECOLIN_RS00220at 43.266 kb on - strand, within ECOLIN_RS00220at 43.266 kb on - strand, within ECOLIN_RS00220at 43.333 kb on + strand, within ECOLIN_RS00220at 43.333 kb on + strand, within ECOLIN_RS00220

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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40,264 + ECOLIN_RS00210 0.31 +0.3
40,265 - ECOLIN_RS00210 0.31 -0.3
40,265 - ECOLIN_RS00210 0.31 +0.2
40,265 - ECOLIN_RS00210 0.31 +0.1
40,319 - ECOLIN_RS00210 0.35 -0.3
40,387 + ECOLIN_RS00210 0.41 -2.7
40,388 - ECOLIN_RS00210 0.41 -1.6
40,389 + ECOLIN_RS00210 0.41 +2.0
40,408 - ECOLIN_RS00210 0.43 -1.6
40,504 - ECOLIN_RS00210 0.51 +0.4
40,684 + ECOLIN_RS00210 0.65 -0.6
40,686 + ECOLIN_RS00210 0.66 +0.3
40,704 + ECOLIN_RS00210 0.67 +0.7
40,705 - ECOLIN_RS00210 0.67 -2.3
40,933 - ECOLIN_RS00210 0.86 -0.1
41,094 - -0.9
41,108 + -0.2
41,108 + -0.2
41,108 + +2.3
41,109 - -0.8
41,109 - +0.8
41,124 + -0.2
41,153 + +1.2
41,816 + ECOLIN_RS00215 0.51 -0.1
41,885 - ECOLIN_RS00215 0.57 +0.1
41,919 - ECOLIN_RS00215 0.60 +0.8
41,932 + ECOLIN_RS00215 0.61 +0.3
42,093 - ECOLIN_RS00215 0.75 +0.3
42,122 - ECOLIN_RS00215 0.78 +0.3
42,637 + ECOLIN_RS00220 0.15 +0.1
42,637 + ECOLIN_RS00220 0.15 +0.9
42,638 - ECOLIN_RS00220 0.15 +0.1
42,638 - ECOLIN_RS00220 0.15 +0.7
42,638 - ECOLIN_RS00220 0.15 +0.4
42,795 - ECOLIN_RS00220 0.26 -0.2
42,940 + ECOLIN_RS00220 0.35 -0.3
42,962 - ECOLIN_RS00220 0.37 -0.8
43,011 - ECOLIN_RS00220 0.40 +0.0
43,012 + ECOLIN_RS00220 0.40 -2.8
43,033 + ECOLIN_RS00220 0.41 -0.7
43,134 - ECOLIN_RS00220 0.48 -0.2
43,265 + ECOLIN_RS00220 0.57 +0.5
43,266 - ECOLIN_RS00220 0.57 +0.6
43,266 - ECOLIN_RS00220 0.57 -1.2
43,333 + ECOLIN_RS00220 0.61 +0.1
43,333 + ECOLIN_RS00220 0.61 -0.1

Or see this region's nucleotide sequence