Experiment: Parafilmed volatile R2A_HEPES agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS08075 and ABIE51_RS08080 are separated by 236 nucleotides ABIE51_RS08080 and ABIE51_RS08085 are separated by 77 nucleotides ABIE51_RS08085 and ABIE51_RS08090 are separated by 198 nucleotides ABIE51_RS08090 and ABIE51_RS08095 are separated by 135 nucleotides
ABIE51_RS08075: ABIE51_RS08075 - TraB/GumN family protein, at 1,718,669 to 1,719,718
_RS08075
ABIE51_RS08080: ABIE51_RS08080 - Rnf electron transport complex subunit RnfB, at 1,719,955 to 1,720,362
_RS08080
ABIE51_RS08085: ABIE51_RS08085 - methionine--tRNA ligase, at 1,720,440 to 1,722,512
_RS08085
ABIE51_RS08090: ABIE51_RS08090 - DUF2147 domain-containing protein, at 1,722,711 to 1,723,160
_RS08090
ABIE51_RS08095: ABIE51_RS08095 - TonB-dependent receptor, at 1,723,296 to 1,726,145
_RS08095
Position (kb)
1720
1721
1722
1723 Strain fitness (log2 ratio)
-1
0
1 at 1719.471 kb on - strand, within ABIE51_RS08075 at 1719.471 kb on - strand, within ABIE51_RS08075 at 1719.471 kb on - strand, within ABIE51_RS08075 at 1719.623 kb on + strand at 1719.623 kb on + strand at 1719.624 kb on - strand at 1719.624 kb on - strand at 1719.716 kb on + strand at 1719.716 kb on + strand at 1719.717 kb on - strand at 1719.717 kb on - strand at 1719.717 kb on - strand at 1719.717 kb on - strand at 1722.576 kb on - strand at 1722.576 kb on - strand at 1722.623 kb on + strand at 1722.623 kb on + strand at 1722.814 kb on + strand, within ABIE51_RS08090 at 1722.814 kb on + strand, within ABIE51_RS08090 at 1722.814 kb on + strand, within ABIE51_RS08090 at 1722.815 kb on - strand, within ABIE51_RS08090 at 1723.015 kb on + strand, within ABIE51_RS08090 at 1723.015 kb on + strand, within ABIE51_RS08090 at 1723.015 kb on + strand, within ABIE51_RS08090 at 1723.016 kb on - strand, within ABIE51_RS08090 at 1723.016 kb on - strand, within ABIE51_RS08090 at 1723.016 kb on - strand, within ABIE51_RS08090 at 1723.016 kb on - strand, within ABIE51_RS08090 at 1723.154 kb on + strand at 1723.155 kb on - strand at 1723.155 kb on - strand at 1723.194 kb on + strand at 1723.194 kb on + strand at 1723.194 kb on + strand at 1723.195 kb on - strand at 1723.195 kb on - strand at 1723.195 kb on - strand at 1723.195 kb on - strand at 1723.328 kb on - strand at 1723.342 kb on + strand at 1723.343 kb on - strand at 1723.351 kb on + strand at 1723.351 kb on + strand at 1723.351 kb on + strand at 1723.352 kb on - strand at 1723.352 kb on - strand at 1723.366 kb on + strand at 1723.367 kb on - strand at 1723.391 kb on - strand at 1723.391 kb on - strand at 1723.471 kb on + strand at 1723.471 kb on + strand at 1723.471 kb on + strand at 1723.472 kb on - strand at 1723.472 kb on - strand at 1723.472 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile R2A_HEPES agar plate with no fungus remove 1,719,471 - ABIE51_RS08075 0.76 -1.7 1,719,471 - ABIE51_RS08075 0.76 +1.0 1,719,471 - ABIE51_RS08075 0.76 -0.1 1,719,623 + -0.3 1,719,623 + +0.3 1,719,624 - -0.8 1,719,624 - -0.7 1,719,716 + -0.2 1,719,716 + +0.2 1,719,717 - -0.1 1,719,717 - -0.1 1,719,717 - +0.1 1,719,717 - +0.2 1,722,576 - +0.1 1,722,576 - +0.1 1,722,623 + -0.3 1,722,623 + +0.4 1,722,814 + ABIE51_RS08090 0.23 -0.4 1,722,814 + ABIE51_RS08090 0.23 +0.1 1,722,814 + ABIE51_RS08090 0.23 +0.1 1,722,815 - ABIE51_RS08090 0.23 -0.4 1,723,015 + ABIE51_RS08090 0.68 -0.1 1,723,015 + ABIE51_RS08090 0.68 +1.1 1,723,015 + ABIE51_RS08090 0.68 -0.5 1,723,016 - ABIE51_RS08090 0.68 +0.5 1,723,016 - ABIE51_RS08090 0.68 -0.7 1,723,016 - ABIE51_RS08090 0.68 -0.7 1,723,016 - ABIE51_RS08090 0.68 -0.0 1,723,154 + +0.5 1,723,155 - -0.3 1,723,155 - +0.3 1,723,194 + -0.3 1,723,194 + -0.7 1,723,194 + +0.6 1,723,195 - -1.1 1,723,195 - -0.2 1,723,195 - +0.4 1,723,195 - +0.2 1,723,328 - +0.9 1,723,342 + -0.3 1,723,343 - -1.0 1,723,351 + +0.6 1,723,351 + -1.0 1,723,351 + -1.2 1,723,352 - -0.2 1,723,352 - +0.3 1,723,366 + -0.0 1,723,367 - +0.2 1,723,391 - +0.0 1,723,391 - +1.2 1,723,471 + +0.0 1,723,471 + -0.4 1,723,471 + -0.2 1,723,472 - +0.1 1,723,472 - -0.2 1,723,472 - -0.6
Or see this region's nucleotide sequence