Experiment: Parafilmed volatile R2A_HEPES agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS15080 and ABIE51_RS15085 are separated by 54 nucleotides ABIE51_RS15085 and ABIE51_RS15090 are separated by 196 nucleotides ABIE51_RS15090 and ABIE51_RS15095 are separated by 60 nucleotides
ABIE51_RS15080: ABIE51_RS15080 - dipeptide epimerase, at 3,277,895 to 3,278,914
_RS15080
ABIE51_RS15085: ABIE51_RS15085 - serine hydrolase, at 3,278,969 to 3,281,014
_RS15085
ABIE51_RS15090: ABIE51_RS15090 - XRE family transcriptional regulator, at 3,281,211 to 3,281,855
_RS15090
ABIE51_RS15095: ABIE51_RS15095 - TMEM43 family protein, at 3,281,916 to 3,282,647
_RS15095
Position (kb)
3278
3279
3280
3281
3282 Strain fitness (log2 ratio)
-1
0
1
2 at 3278.156 kb on + strand, within ABIE51_RS15080 at 3278.156 kb on + strand, within ABIE51_RS15080 at 3278.156 kb on + strand, within ABIE51_RS15080 at 3278.156 kb on + strand, within ABIE51_RS15080 at 3278.157 kb on - strand, within ABIE51_RS15080 at 3278.157 kb on - strand, within ABIE51_RS15080 at 3278.157 kb on - strand, within ABIE51_RS15080 at 3278.481 kb on + strand, within ABIE51_RS15080 at 3278.482 kb on - strand, within ABIE51_RS15080 at 3278.751 kb on + strand, within ABIE51_RS15080 at 3278.752 kb on - strand, within ABIE51_RS15080 at 3278.929 kb on + strand at 3278.974 kb on - strand at 3278.974 kb on - strand at 3279.168 kb on + strand at 3279.168 kb on + strand at 3279.169 kb on - strand at 3279.654 kb on + strand, within ABIE51_RS15085 at 3279.654 kb on + strand, within ABIE51_RS15085 at 3279.655 kb on - strand, within ABIE51_RS15085 at 3279.655 kb on - strand, within ABIE51_RS15085 at 3279.655 kb on - strand, within ABIE51_RS15085 at 3279.655 kb on - strand, within ABIE51_RS15085 at 3279.700 kb on - strand, within ABIE51_RS15085 at 3279.861 kb on + strand, within ABIE51_RS15085 at 3279.861 kb on + strand, within ABIE51_RS15085 at 3279.861 kb on + strand, within ABIE51_RS15085 at 3279.861 kb on + strand, within ABIE51_RS15085 at 3279.862 kb on - strand, within ABIE51_RS15085 at 3279.862 kb on - strand, within ABIE51_RS15085 at 3279.963 kb on + strand, within ABIE51_RS15085 at 3279.963 kb on + strand, within ABIE51_RS15085 at 3279.964 kb on - strand, within ABIE51_RS15085 at 3280.149 kb on + strand, within ABIE51_RS15085 at 3280.149 kb on + strand, within ABIE51_RS15085 at 3280.149 kb on + strand, within ABIE51_RS15085 at 3280.150 kb on - strand, within ABIE51_RS15085 at 3280.150 kb on - strand, within ABIE51_RS15085 at 3280.155 kb on + strand, within ABIE51_RS15085 at 3280.188 kb on + strand, within ABIE51_RS15085 at 3280.188 kb on + strand, within ABIE51_RS15085 at 3280.188 kb on + strand, within ABIE51_RS15085 at 3280.189 kb on - strand, within ABIE51_RS15085 at 3280.189 kb on - strand, within ABIE51_RS15085 at 3280.285 kb on - strand, within ABIE51_RS15085 at 3280.323 kb on + strand, within ABIE51_RS15085 at 3280.323 kb on + strand, within ABIE51_RS15085 at 3280.324 kb on - strand, within ABIE51_RS15085 at 3280.324 kb on - strand, within ABIE51_RS15085 at 3280.353 kb on + strand, within ABIE51_RS15085 at 3280.377 kb on + strand, within ABIE51_RS15085 at 3280.378 kb on - strand, within ABIE51_RS15085 at 3280.378 kb on - strand, within ABIE51_RS15085 at 3280.545 kb on + strand, within ABIE51_RS15085 at 3280.546 kb on - strand, within ABIE51_RS15085 at 3280.546 kb on - strand, within ABIE51_RS15085 at 3280.546 kb on - strand, within ABIE51_RS15085 at 3281.355 kb on + strand, within ABIE51_RS15090 at 3281.356 kb on - strand, within ABIE51_RS15090 at 3281.356 kb on - strand, within ABIE51_RS15090 at 3281.356 kb on - strand, within ABIE51_RS15090 at 3281.356 kb on - strand, within ABIE51_RS15090 at 3281.356 kb on - strand, within ABIE51_RS15090 at 3281.356 kb on - strand, within ABIE51_RS15090 at 3281.502 kb on + strand, within ABIE51_RS15090 at 3281.502 kb on + strand, within ABIE51_RS15090 at 3281.503 kb on - strand, within ABIE51_RS15090 at 3281.503 kb on - strand, within ABIE51_RS15090 at 3281.508 kb on + strand, within ABIE51_RS15090 at 3281.508 kb on + strand, within ABIE51_RS15090 at 3281.508 kb on + strand, within ABIE51_RS15090 at 3281.508 kb on + strand, within ABIE51_RS15090 at 3281.509 kb on - strand, within ABIE51_RS15090 at 3281.509 kb on - strand, within ABIE51_RS15090 at 3281.509 kb on - strand, within ABIE51_RS15090 at 3281.509 kb on - strand, within ABIE51_RS15090 at 3281.509 kb on - strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.514 kb on + strand, within ABIE51_RS15090 at 3281.515 kb on - strand, within ABIE51_RS15090 at 3281.515 kb on - strand, within ABIE51_RS15090 at 3281.515 kb on - strand, within ABIE51_RS15090 at 3281.515 kb on - strand, within ABIE51_RS15090 at 3281.515 kb on - strand, within ABIE51_RS15090 at 3281.515 kb on - strand, within ABIE51_RS15090 at 3281.623 kb on - strand, within ABIE51_RS15090 at 3281.623 kb on - strand, within ABIE51_RS15090 at 3281.623 kb on - strand, within ABIE51_RS15090 at 3281.667 kb on + strand, within ABIE51_RS15090 at 3281.667 kb on + strand, within ABIE51_RS15090 at 3281.667 kb on + strand, within ABIE51_RS15090 at 3281.667 kb on + strand, within ABIE51_RS15090 at 3281.667 kb on + strand, within ABIE51_RS15090 at 3281.668 kb on - strand, within ABIE51_RS15090 at 3281.668 kb on - strand, within ABIE51_RS15090 at 3281.668 kb on - strand, within ABIE51_RS15090 at 3281.668 kb on - strand, within ABIE51_RS15090 at 3281.703 kb on + strand, within ABIE51_RS15090 at 3281.704 kb on - strand, within ABIE51_RS15090 at 3281.704 kb on - strand, within ABIE51_RS15090 at 3281.704 kb on - strand, within ABIE51_RS15090 at 3281.704 kb on - strand, within ABIE51_RS15090 at 3281.704 kb on - strand, within ABIE51_RS15090 at 3281.730 kb on + strand, within ABIE51_RS15090 at 3281.730 kb on + strand, within ABIE51_RS15090 at 3281.731 kb on - strand, within ABIE51_RS15090 at 3281.731 kb on - strand, within ABIE51_RS15090 at 3281.871 kb on + strand at 3281.872 kb on - strand at 3281.872 kb on - strand at 3281.872 kb on - strand at 3281.917 kb on + strand at 3281.918 kb on - strand at 3281.964 kb on + strand at 3281.964 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile R2A_HEPES agar plate with no fungus remove 3,278,156 + ABIE51_RS15080 0.26 +0.1 3,278,156 + ABIE51_RS15080 0.26 +1.7 3,278,156 + ABIE51_RS15080 0.26 -0.9 3,278,156 + ABIE51_RS15080 0.26 +0.5 3,278,157 - ABIE51_RS15080 0.26 +0.1 3,278,157 - ABIE51_RS15080 0.26 +0.5 3,278,157 - ABIE51_RS15080 0.26 +0.0 3,278,481 + ABIE51_RS15080 0.57 -0.0 3,278,482 - ABIE51_RS15080 0.58 -0.4 3,278,751 + ABIE51_RS15080 0.84 +0.9 3,278,752 - ABIE51_RS15080 0.84 +0.1 3,278,929 + -0.2 3,278,974 - -0.2 3,278,974 - -0.3 3,279,168 + -0.1 3,279,168 + -0.1 3,279,169 - -1.0 3,279,654 + ABIE51_RS15085 0.33 -0.6 3,279,654 + ABIE51_RS15085 0.33 +0.4 3,279,655 - ABIE51_RS15085 0.34 -0.2 3,279,655 - ABIE51_RS15085 0.34 +1.4 3,279,655 - ABIE51_RS15085 0.34 +1.1 3,279,655 - ABIE51_RS15085 0.34 +0.4 3,279,700 - ABIE51_RS15085 0.36 -0.2 3,279,861 + ABIE51_RS15085 0.44 -0.2 3,279,861 + ABIE51_RS15085 0.44 +0.3 3,279,861 + ABIE51_RS15085 0.44 +0.6 3,279,861 + ABIE51_RS15085 0.44 -0.4 3,279,862 - ABIE51_RS15085 0.44 +0.2 3,279,862 - ABIE51_RS15085 0.44 +0.6 3,279,963 + ABIE51_RS15085 0.49 -0.3 3,279,963 + ABIE51_RS15085 0.49 +0.7 3,279,964 - ABIE51_RS15085 0.49 -0.2 3,280,149 + ABIE51_RS15085 0.58 +0.3 3,280,149 + ABIE51_RS15085 0.58 -0.2 3,280,149 + ABIE51_RS15085 0.58 -0.1 3,280,150 - ABIE51_RS15085 0.58 -0.4 3,280,150 - ABIE51_RS15085 0.58 +0.3 3,280,155 + ABIE51_RS15085 0.58 -0.2 3,280,188 + ABIE51_RS15085 0.60 -0.7 3,280,188 + ABIE51_RS15085 0.60 -0.6 3,280,188 + ABIE51_RS15085 0.60 -0.6 3,280,189 - ABIE51_RS15085 0.60 -0.4 3,280,189 - ABIE51_RS15085 0.60 +0.7 3,280,285 - ABIE51_RS15085 0.64 -0.5 3,280,323 + ABIE51_RS15085 0.66 -0.1 3,280,323 + ABIE51_RS15085 0.66 +0.0 3,280,324 - ABIE51_RS15085 0.66 -0.8 3,280,324 - ABIE51_RS15085 0.66 +0.3 3,280,353 + ABIE51_RS15085 0.68 +1.3 3,280,377 + ABIE51_RS15085 0.69 +1.1 3,280,378 - ABIE51_RS15085 0.69 +0.0 3,280,378 - ABIE51_RS15085 0.69 -0.2 3,280,545 + ABIE51_RS15085 0.77 -0.9 3,280,546 - ABIE51_RS15085 0.77 +0.5 3,280,546 - ABIE51_RS15085 0.77 -0.7 3,280,546 - ABIE51_RS15085 0.77 +0.2 3,281,355 + ABIE51_RS15090 0.22 +0.1 3,281,356 - ABIE51_RS15090 0.22 +0.2 3,281,356 - ABIE51_RS15090 0.22 +0.3 3,281,356 - ABIE51_RS15090 0.22 +0.1 3,281,356 - ABIE51_RS15090 0.22 +0.3 3,281,356 - ABIE51_RS15090 0.22 -0.6 3,281,356 - ABIE51_RS15090 0.22 -0.1 3,281,502 + ABIE51_RS15090 0.45 -0.7 3,281,502 + ABIE51_RS15090 0.45 +0.2 3,281,503 - ABIE51_RS15090 0.45 -0.6 3,281,503 - ABIE51_RS15090 0.45 +2.1 3,281,508 + ABIE51_RS15090 0.46 +0.1 3,281,508 + ABIE51_RS15090 0.46 -0.6 3,281,508 + ABIE51_RS15090 0.46 -0.3 3,281,508 + ABIE51_RS15090 0.46 +0.3 3,281,509 - ABIE51_RS15090 0.46 +0.1 3,281,509 - ABIE51_RS15090 0.46 +0.9 3,281,509 - ABIE51_RS15090 0.46 +0.3 3,281,509 - ABIE51_RS15090 0.46 -0.4 3,281,509 - ABIE51_RS15090 0.46 -0.2 3,281,514 + ABIE51_RS15090 0.47 +0.3 3,281,514 + ABIE51_RS15090 0.47 -0.4 3,281,514 + ABIE51_RS15090 0.47 -0.1 3,281,514 + ABIE51_RS15090 0.47 -0.3 3,281,514 + ABIE51_RS15090 0.47 -0.3 3,281,514 + ABIE51_RS15090 0.47 -0.2 3,281,514 + ABIE51_RS15090 0.47 -0.1 3,281,514 + ABIE51_RS15090 0.47 +1.4 3,281,515 - ABIE51_RS15090 0.47 -0.6 3,281,515 - ABIE51_RS15090 0.47 -0.4 3,281,515 - ABIE51_RS15090 0.47 -0.2 3,281,515 - ABIE51_RS15090 0.47 +0.8 3,281,515 - ABIE51_RS15090 0.47 -0.3 3,281,515 - ABIE51_RS15090 0.47 -0.8 3,281,623 - ABIE51_RS15090 0.64 -0.3 3,281,623 - ABIE51_RS15090 0.64 -0.5 3,281,623 - ABIE51_RS15090 0.64 +0.2 3,281,667 + ABIE51_RS15090 0.71 +0.3 3,281,667 + ABIE51_RS15090 0.71 -0.0 3,281,667 + ABIE51_RS15090 0.71 +0.7 3,281,667 + ABIE51_RS15090 0.71 -0.4 3,281,667 + ABIE51_RS15090 0.71 +0.8 3,281,668 - ABIE51_RS15090 0.71 -0.5 3,281,668 - ABIE51_RS15090 0.71 +0.6 3,281,668 - ABIE51_RS15090 0.71 -0.4 3,281,668 - ABIE51_RS15090 0.71 -0.1 3,281,703 + ABIE51_RS15090 0.76 -0.3 3,281,704 - ABIE51_RS15090 0.76 -0.1 3,281,704 - ABIE51_RS15090 0.76 -0.4 3,281,704 - ABIE51_RS15090 0.76 -0.1 3,281,704 - ABIE51_RS15090 0.76 -0.7 3,281,704 - ABIE51_RS15090 0.76 +0.1 3,281,730 + ABIE51_RS15090 0.80 +0.3 3,281,730 + ABIE51_RS15090 0.80 +0.6 3,281,731 - ABIE51_RS15090 0.81 -0.5 3,281,731 - ABIE51_RS15090 0.81 +0.5 3,281,871 + -0.2 3,281,872 - -0.3 3,281,872 - -0.3 3,281,872 - -0.8 3,281,917 + +2.1 3,281,918 - +0.1 3,281,964 + -1.2 3,281,964 + -0.5
Or see this region's nucleotide sequence