Experiment: Parafilmed volatile R2A_HEPES agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS14340 and ABIE51_RS14345 are separated by 352 nucleotides ABIE51_RS14345 and ABIE51_RS14350 are separated by 705 nucleotides
ABIE51_RS14340: ABIE51_RS14340 - pirin family protein, at 3,100,276 to 3,101,136
_RS14340
ABIE51_RS14345: ABIE51_RS14345 - aminomethyl-transferring glycine dehydrogenase, at 3,101,489 to 3,104,380
_RS14345
ABIE51_RS14350: ABIE51_RS14350 - OmpA family protein, at 3,105,086 to 3,105,919
_RS14350
Position (kb)
3101
3102
3103
3104
3105 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 3100.777 kb on + strand, within ABIE51_RS14340 at 3100.777 kb on + strand, within ABIE51_RS14340 at 3100.778 kb on - strand, within ABIE51_RS14340 at 3100.778 kb on - strand, within ABIE51_RS14340 at 3100.792 kb on + strand, within ABIE51_RS14340 at 3100.792 kb on + strand, within ABIE51_RS14340 at 3100.792 kb on + strand, within ABIE51_RS14340 at 3100.793 kb on - strand, within ABIE51_RS14340 at 3100.862 kb on - strand, within ABIE51_RS14340 at 3101.287 kb on - strand at 3101.759 kb on + strand at 3101.760 kb on - strand at 3101.760 kb on - strand at 3101.760 kb on - strand at 3101.763 kb on - strand at 3102.016 kb on - strand, within ABIE51_RS14345 at 3102.173 kb on + strand, within ABIE51_RS14345 at 3102.173 kb on + strand, within ABIE51_RS14345 at 3102.174 kb on - strand, within ABIE51_RS14345 at 3102.174 kb on - strand, within ABIE51_RS14345 at 3102.365 kb on + strand, within ABIE51_RS14345 at 3102.366 kb on - strand, within ABIE51_RS14345 at 3102.366 kb on - strand, within ABIE51_RS14345 at 3102.530 kb on + strand, within ABIE51_RS14345 at 3102.539 kb on + strand, within ABIE51_RS14345 at 3102.938 kb on + strand, within ABIE51_RS14345 at 3102.938 kb on + strand, within ABIE51_RS14345 at 3103.100 kb on + strand, within ABIE51_RS14345 at 3103.223 kb on + strand, within ABIE51_RS14345 at 3103.223 kb on + strand, within ABIE51_RS14345 at 3103.392 kb on - strand, within ABIE51_RS14345 at 3103.502 kb on + strand, within ABIE51_RS14345 at 3103.502 kb on + strand, within ABIE51_RS14345 at 3103.502 kb on + strand, within ABIE51_RS14345 at 3103.503 kb on - strand, within ABIE51_RS14345 at 3103.784 kb on + strand, within ABIE51_RS14345 at 3103.850 kb on + strand, within ABIE51_RS14345 at 3103.851 kb on - strand, within ABIE51_RS14345 at 3103.851 kb on - strand, within ABIE51_RS14345 at 3103.851 kb on - strand, within ABIE51_RS14345 at 3103.890 kb on - strand, within ABIE51_RS14345 at 3104.276 kb on + strand at 3104.276 kb on + strand at 3104.310 kb on - strand at 3104.310 kb on - strand at 3104.354 kb on + strand at 3104.355 kb on - strand at 3104.355 kb on - strand at 3104.378 kb on + strand at 3104.565 kb on + strand at 3104.848 kb on - strand at 3105.084 kb on - strand at 3105.240 kb on + strand, within ABIE51_RS14350 at 3105.240 kb on + strand, within ABIE51_RS14350 at 3105.285 kb on + strand, within ABIE51_RS14350 at 3105.295 kb on + strand, within ABIE51_RS14350
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile R2A_HEPES agar plate with no fungus remove 3,100,777 + ABIE51_RS14340 0.58 +0.2 3,100,777 + ABIE51_RS14340 0.58 -0.5 3,100,778 - ABIE51_RS14340 0.58 -0.7 3,100,778 - ABIE51_RS14340 0.58 -0.3 3,100,792 + ABIE51_RS14340 0.60 -0.3 3,100,792 + ABIE51_RS14340 0.60 -0.4 3,100,792 + ABIE51_RS14340 0.60 +1.3 3,100,793 - ABIE51_RS14340 0.60 +0.5 3,100,862 - ABIE51_RS14340 0.68 +0.7 3,101,287 - -0.9 3,101,759 + -1.8 3,101,760 - -0.2 3,101,760 - -2.7 3,101,760 - -2.4 3,101,763 - -1.4 3,102,016 - ABIE51_RS14345 0.18 -1.3 3,102,173 + ABIE51_RS14345 0.24 -1.6 3,102,173 + ABIE51_RS14345 0.24 -1.2 3,102,174 - ABIE51_RS14345 0.24 -1.4 3,102,174 - ABIE51_RS14345 0.24 -2.1 3,102,365 + ABIE51_RS14345 0.30 -1.9 3,102,366 - ABIE51_RS14345 0.30 -0.9 3,102,366 - ABIE51_RS14345 0.30 -2.6 3,102,530 + ABIE51_RS14345 0.36 -0.7 3,102,539 + ABIE51_RS14345 0.36 -0.5 3,102,938 + ABIE51_RS14345 0.50 -1.7 3,102,938 + ABIE51_RS14345 0.50 +0.8 3,103,100 + ABIE51_RS14345 0.56 -2.0 3,103,223 + ABIE51_RS14345 0.60 -2.7 3,103,223 + ABIE51_RS14345 0.60 -1.4 3,103,392 - ABIE51_RS14345 0.66 -0.0 3,103,502 + ABIE51_RS14345 0.70 -0.3 3,103,502 + ABIE51_RS14345 0.70 -3.8 3,103,502 + ABIE51_RS14345 0.70 -0.4 3,103,503 - ABIE51_RS14345 0.70 -0.1 3,103,784 + ABIE51_RS14345 0.79 -1.2 3,103,850 + ABIE51_RS14345 0.82 -1.0 3,103,851 - ABIE51_RS14345 0.82 +0.5 3,103,851 - ABIE51_RS14345 0.82 -1.5 3,103,851 - ABIE51_RS14345 0.82 -1.1 3,103,890 - ABIE51_RS14345 0.83 -0.7 3,104,276 + -1.5 3,104,276 + -2.2 3,104,310 - -1.4 3,104,310 - -2.7 3,104,354 + -1.7 3,104,355 - +0.9 3,104,355 - +0.1 3,104,378 + +0.2 3,104,565 + -0.0 3,104,848 - +0.7 3,105,084 - -0.6 3,105,240 + ABIE51_RS14350 0.18 -0.2 3,105,240 + ABIE51_RS14350 0.18 +0.1 3,105,285 + ABIE51_RS14350 0.24 -1.3 3,105,295 + ABIE51_RS14350 0.25 -0.3
Or see this region's nucleotide sequence