Experiment: Parafilmed volatile R2A agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS06200 and ABIE51_RS06205 are separated by 29 nucleotides ABIE51_RS06205 and ABIE51_RS06210 are separated by 39 nucleotides ABIE51_RS06210 and ABIE51_RS06215 are separated by 3 nucleotides ABIE51_RS06215 and ABIE51_RS06220 overlap by 4 nucleotides
ABIE51_RS06200: ABIE51_RS06200 - glutamate--tRNA ligase, at 1,325,692 to 1,327,099
_RS06200
ABIE51_RS06205: ABIE51_RS06205 - transcriptional repressor, at 1,327,129 to 1,327,620
_RS06205
ABIE51_RS06210: ABIE51_RS06210 - hypothetical protein, at 1,327,660 to 1,328,088
_RS06210
ABIE51_RS06215: ABIE51_RS06215 - cupin domain-containing protein, at 1,328,092 to 1,328,451
_RS06215
ABIE51_RS06220: ABIE51_RS06220 - MFS transporter, at 1,328,448 to 1,329,710
_RS06220
Position (kb)
1327
1328 Strain fitness (log2 ratio)
-1
0
1 at 1327.661 kb on + strand at 1327.661 kb on + strand at 1327.662 kb on - strand at 1327.662 kb on - strand at 1327.667 kb on + strand at 1327.667 kb on + strand at 1327.667 kb on + strand at 1327.667 kb on + strand at 1327.667 kb on + strand at 1327.667 kb on + strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.668 kb on - strand at 1327.733 kb on + strand, within ABIE51_RS06210 at 1327.734 kb on - strand, within ABIE51_RS06210 at 1327.734 kb on - strand, within ABIE51_RS06210 at 1327.921 kb on + strand, within ABIE51_RS06210 at 1327.921 kb on + strand, within ABIE51_RS06210 at 1327.922 kb on - strand, within ABIE51_RS06210 at 1328.030 kb on + strand, within ABIE51_RS06210 at 1328.064 kb on + strand at 1328.064 kb on + strand at 1328.077 kb on + strand at 1328.078 kb on - strand at 1328.078 kb on - strand at 1328.551 kb on + strand at 1328.567 kb on - strand at 1328.567 kb on - strand at 1328.567 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile R2A agar plate with no fungus remove 1,327,661 + -0.1 1,327,661 + -0.1 1,327,662 - -0.2 1,327,662 - +0.5 1,327,667 + -0.2 1,327,667 + +0.5 1,327,667 + +1.3 1,327,667 + -0.6 1,327,667 + +0.5 1,327,667 + +0.0 1,327,668 - +0.5 1,327,668 - +0.6 1,327,668 - -0.2 1,327,668 - +0.1 1,327,668 - +0.7 1,327,668 - -0.2 1,327,668 - +0.1 1,327,668 - +0.2 1,327,668 - +0.1 1,327,668 - +0.1 1,327,668 - +0.4 1,327,668 - -0.1 1,327,668 - -0.1 1,327,733 + ABIE51_RS06210 0.17 +0.3 1,327,734 - ABIE51_RS06210 0.17 -1.0 1,327,734 - ABIE51_RS06210 0.17 +0.9 1,327,921 + ABIE51_RS06210 0.61 +0.1 1,327,921 + ABIE51_RS06210 0.61 +0.5 1,327,922 - ABIE51_RS06210 0.61 +0.0 1,328,030 + ABIE51_RS06210 0.86 +0.1 1,328,064 + -0.3 1,328,064 + -0.4 1,328,077 + +0.0 1,328,078 - -0.0 1,328,078 - -0.5 1,328,551 + -0.7 1,328,567 - -0.2 1,328,567 - +0.1 1,328,567 - -0.1
Or see this region's nucleotide sequence