Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS21975 and ECOLIN_RS21980 are separated by 117 nucleotides ECOLIN_RS21980 and ECOLIN_RS33090 are separated by 168 nucleotides ECOLIN_RS33090 and ECOLIN_RS21990 are separated by 74 nucleotides ECOLIN_RS21990 and ECOLIN_RS33095 overlap by 4 nucleotides
ECOLIN_RS21970: ECOLIN_RS21970 - biotin transporter, at 4,413,461 to 4,414,360
_RS21970
ECOLIN_RS21975: ECOLIN_RS21975 - HTH-type transcriptional regulator MetR, at 4,414,248 to 4,415,201
_RS21975
ECOLIN_RS21980: ECOLIN_RS21980 - 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase, at 4,415,319 to 4,417,580
_RS21980
ECOLIN_RS33090: ECOLIN_RS33090 - hypothetical protein, at 4,417,749 to 4,417,994
_RS33090
ECOLIN_RS21990: ECOLIN_RS21990 - hypothetical protein, at 4,418,069 to 4,418,326
_RS21990
ECOLIN_RS33095: ECOLIN_RS33095 - type VI secretion system tube protein TssD, at 4,418,323 to 4,419,456
_RS33095
Position (kb) 4415
4416
4417
4418 Strain fitness (log2 ratio) -2
-1
0
1 at 4415.517 kb on + strand at 4415.527 kb on + strand at 4415.527 kb on + strand at 4415.527 kb on + strand at 4415.527 kb on + strand at 4415.527 kb on + strand at 4415.676 kb on - strand, within ECOLIN_RS21980 at 4415.691 kb on + strand, within ECOLIN_RS21980 at 4415.691 kb on + strand, within ECOLIN_RS21980 at 4415.691 kb on + strand, within ECOLIN_RS21980 at 4415.692 kb on - strand, within ECOLIN_RS21980 at 4415.813 kb on + strand, within ECOLIN_RS21980 at 4415.813 kb on + strand, within ECOLIN_RS21980 at 4415.992 kb on - strand, within ECOLIN_RS21980 at 4416.046 kb on - strand, within ECOLIN_RS21980 at 4416.046 kb on - strand, within ECOLIN_RS21980 at 4416.119 kb on - strand, within ECOLIN_RS21980 at 4416.119 kb on - strand, within ECOLIN_RS21980 at 4416.188 kb on + strand, within ECOLIN_RS21980 at 4416.189 kb on - strand, within ECOLIN_RS21980 at 4416.479 kb on - strand, within ECOLIN_RS21980 at 4416.483 kb on - strand, within ECOLIN_RS21980 at 4416.660 kb on - strand, within ECOLIN_RS21980 at 4416.684 kb on - strand, within ECOLIN_RS21980 at 4416.760 kb on + strand, within ECOLIN_RS21980 at 4416.760 kb on + strand, within ECOLIN_RS21980 at 4416.780 kb on - strand, within ECOLIN_RS21980 at 4416.990 kb on - strand, within ECOLIN_RS21980 at 4417.586 kb on - strand at 4417.629 kb on + strand at 4417.680 kb on - strand at 4417.746 kb on - strand at 4417.750 kb on + strand at 4417.814 kb on + strand, within ECOLIN_RS33090 at 4417.942 kb on - strand, within ECOLIN_RS33090 at 4417.944 kb on - strand, within ECOLIN_RS33090 at 4417.958 kb on + strand, within ECOLIN_RS33090 at 4418.157 kb on - strand, within ECOLIN_RS21990 at 4418.234 kb on - strand, within ECOLIN_RS21990 at 4418.234 kb on - strand, within ECOLIN_RS21990 at 4418.449 kb on + strand, within ECOLIN_RS33095 at 4418.487 kb on + strand, within ECOLIN_RS33095
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 4,415,517 + -0.2 4,415,527 + -0.0 4,415,527 + +0.0 4,415,527 + +0.0 4,415,527 + +0.0 4,415,527 + -0.3 4,415,676 - ECOLIN_RS21980 0.16 -0.2 4,415,691 + ECOLIN_RS21980 0.16 +0.7 4,415,691 + ECOLIN_RS21980 0.16 -0.1 4,415,691 + ECOLIN_RS21980 0.16 +0.1 4,415,692 - ECOLIN_RS21980 0.16 +0.0 4,415,813 + ECOLIN_RS21980 0.22 -0.2 4,415,813 + ECOLIN_RS21980 0.22 +0.2 4,415,992 - ECOLIN_RS21980 0.30 +0.3 4,416,046 - ECOLIN_RS21980 0.32 -0.0 4,416,046 - ECOLIN_RS21980 0.32 +0.3 4,416,119 - ECOLIN_RS21980 0.35 -0.1 4,416,119 - ECOLIN_RS21980 0.35 +0.2 4,416,188 + ECOLIN_RS21980 0.38 -0.2 4,416,189 - ECOLIN_RS21980 0.38 -0.0 4,416,479 - ECOLIN_RS21980 0.51 -0.1 4,416,483 - ECOLIN_RS21980 0.51 -0.3 4,416,660 - ECOLIN_RS21980 0.59 +0.2 4,416,684 - ECOLIN_RS21980 0.60 +1.0 4,416,760 + ECOLIN_RS21980 0.64 +0.1 4,416,760 + ECOLIN_RS21980 0.64 -0.2 4,416,780 - ECOLIN_RS21980 0.65 -0.0 4,416,990 - ECOLIN_RS21980 0.74 -0.0 4,417,586 - +0.1 4,417,629 + -0.1 4,417,680 - -0.1 4,417,746 - -0.7 4,417,750 + +0.3 4,417,814 + ECOLIN_RS33090 0.26 +0.3 4,417,942 - ECOLIN_RS33090 0.78 +0.3 4,417,944 - ECOLIN_RS33090 0.79 -0.3 4,417,958 + ECOLIN_RS33090 0.85 -1.8 4,418,157 - ECOLIN_RS21990 0.34 +0.1 4,418,234 - ECOLIN_RS21990 0.64 +0.6 4,418,234 - ECOLIN_RS21990 0.64 -0.0 4,418,449 + ECOLIN_RS33095 0.11 +0.1 4,418,487 + ECOLIN_RS33095 0.14 +0.8
Or see this region's nucleotide sequence