Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS10760 and ECOLIN_RS32340 are separated by 114 nucleotides ECOLIN_RS32340 and ECOLIN_RS10770 are separated by 45 nucleotides ECOLIN_RS10770 and ECOLIN_RS10775 are separated by 144 nucleotides ECOLIN_RS10775 and ECOLIN_RS10780 are separated by 20 nucleotides
ECOLIN_RS10760: ECOLIN_RS10760 - protein-glutamate O-methyltransferase CheR, at 2,095,495 to 2,096,355
_RS10760
ECOLIN_RS32340: ECOLIN_RS32340 - Tar ligand binding domain-containing protein, at 2,096,470 to 2,096,600
_RS32340
ECOLIN_RS10770: ECOLIN_RS10770 - methyl-accepting chemotaxis protein II, at 2,096,646 to 2,098,307
_RS10770
ECOLIN_RS10775: ECOLIN_RS10775 - chemotaxis protein CheW, at 2,098,452 to 2,098,955
_RS10775
ECOLIN_RS10780: ECOLIN_RS10780 - chemotaxis protein CheA, at 2,098,976 to 2,100,940
_RS10780
Position (kb)
2096
2097
2098
2099 Strain fitness (log2 ratio)
-1
0
1 at 2096.320 kb on + strand at 2096.355 kb on + strand at 2097.657 kb on - strand, within ECOLIN_RS10770 at 2098.793 kb on + strand, within ECOLIN_RS10775 at 2098.996 kb on + strand at 2099.105 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 2,096,320 + -0.7 2,096,355 + +0.1 2,097,657 - ECOLIN_RS10770 0.61 +0.3 2,098,793 + ECOLIN_RS10775 0.68 -0.7 2,098,996 + -0.2 2,099,105 - +0.9
Or see this region's nucleotide sequence