Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS32640 and ECOLIN_RS00345 are separated by 127 nucleotides ECOLIN_RS00345 and ECOLIN_RS00350 are separated by 6 nucleotides ECOLIN_RS00350 and ECOLIN_RS00355 are separated by 154 nucleotides
ECOLIN_RS32640: ECOLIN_RS32640 - IS630 family transposase, at 72,597 to 72,772
_RS32640
ECOLIN_RS00345: ECOLIN_RS00345 - hypothetical protein, at 72,900 to 73,747
_RS00345
ECOLIN_RS00350: ECOLIN_RS00350 - hypothetical protein, at 73,754 to 74,176
_RS00350
ECOLIN_RS00355: ECOLIN_RS00355 - DedA family protein, at 74,331 to 75,095
_RS00355
Position (kb) 73
74
75 Strain fitness (log2 ratio) -2
-1
0
1
2 at 72.874 kb on + strand at 73.937 kb on + strand, within ECOLIN_RS00350 at 74.068 kb on + strand, within ECOLIN_RS00350 at 74.068 kb on + strand, within ECOLIN_RS00350 at 74.068 kb on + strand, within ECOLIN_RS00350 at 74.282 kb on + strand at 74.354 kb on + strand at 74.366 kb on + strand at 74.366 kb on + strand at 74.373 kb on + strand at 74.373 kb on + strand at 74.491 kb on + strand, within ECOLIN_RS00355 at 74.491 kb on + strand, within ECOLIN_RS00355 at 74.962 kb on - strand, within ECOLIN_RS00355 at 75.060 kb on + strand at 75.060 kb on + strand at 75.169 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 72,874 + +1.8 73,937 + ECOLIN_RS00350 0.43 +0.1 74,068 + ECOLIN_RS00350 0.74 -0.7 74,068 + ECOLIN_RS00350 0.74 +0.0 74,068 + ECOLIN_RS00350 0.74 -0.2 74,282 + +1.5 74,354 + +0.0 74,366 + -0.1 74,366 + +0.1 74,373 + +0.6 74,373 + -0.2 74,491 + ECOLIN_RS00355 0.21 -0.2 74,491 + ECOLIN_RS00355 0.21 +0.9 74,962 - ECOLIN_RS00355 0.82 +0.3 75,060 + +0.0 75,060 + -0.3 75,169 - -1.9
Or see this region's nucleotide sequence