Experiment: Parafilmed volatile R2A agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS06900 and ABIE51_RS06905 are separated by 86 nucleotides ABIE51_RS06905 and ABIE51_RS06910 are separated by 27 nucleotides
ABIE51_RS06900: ABIE51_RS06900 - outer membrane protein assembly factor BamA, at 1,482,487 to 1,484,973
_RS06900
ABIE51_RS06905: ABIE51_RS06905 - RIP metalloprotease RseP, at 1,485,060 to 1,486,424
_RS06905
ABIE51_RS06910: ABIE51_RS06910 - 1-deoxy-D-xylulose-5-phosphate reductoisomerase, at 1,486,452 to 1,487,619
_RS06910
Position (kb)
1485
1486
1487 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1484.977 kb on + strand at 1484.978 kb on - strand at 1484.978 kb on - strand at 1484.978 kb on - strand at 1484.978 kb on - strand at 1485.091 kb on + strand at 1485.092 kb on - strand at 1485.092 kb on - strand at 1485.092 kb on - strand at 1485.136 kb on + strand at 1485.136 kb on + strand at 1485.136 kb on + strand at 1485.136 kb on + strand at 1485.136 kb on + strand at 1485.136 kb on + strand at 1485.137 kb on - strand at 1485.137 kb on - strand at 1485.137 kb on - strand at 1485.137 kb on - strand at 1485.192 kb on - strand at 1485.196 kb on + strand at 1485.196 kb on + strand at 1485.196 kb on + strand at 1485.196 kb on + strand at 1485.196 kb on + strand at 1485.197 kb on - strand, within ABIE51_RS06905 at 1485.197 kb on - strand, within ABIE51_RS06905 at 1485.197 kb on - strand, within ABIE51_RS06905 at 1485.197 kb on - strand, within ABIE51_RS06905 at 1485.197 kb on - strand, within ABIE51_RS06905 at 1485.199 kb on + strand, within ABIE51_RS06905 at 1485.199 kb on + strand, within ABIE51_RS06905 at 1485.199 kb on + strand, within ABIE51_RS06905 at 1485.199 kb on + strand, within ABIE51_RS06905 at 1485.200 kb on - strand, within ABIE51_RS06905 at 1485.200 kb on - strand, within ABIE51_RS06905 at 1485.200 kb on - strand, within ABIE51_RS06905 at 1485.200 kb on - strand, within ABIE51_RS06905 at 1485.200 kb on - strand, within ABIE51_RS06905 at 1485.366 kb on + strand, within ABIE51_RS06905 at 1485.463 kb on + strand, within ABIE51_RS06905 at 1485.464 kb on - strand, within ABIE51_RS06905 at 1485.775 kb on + strand, within ABIE51_RS06905 at 1485.775 kb on + strand, within ABIE51_RS06905 at 1485.775 kb on + strand, within ABIE51_RS06905 at 1485.775 kb on + strand, within ABIE51_RS06905 at 1485.776 kb on - strand, within ABIE51_RS06905 at 1485.795 kb on + strand, within ABIE51_RS06905 at 1485.795 kb on + strand, within ABIE51_RS06905 at 1485.795 kb on + strand, within ABIE51_RS06905 at 1486.219 kb on + strand, within ABIE51_RS06905 at 1486.220 kb on - strand, within ABIE51_RS06905 at 1486.220 kb on - strand, within ABIE51_RS06905 at 1486.250 kb on - strand, within ABIE51_RS06905 at 1486.345 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile R2A agar plate with no fungus remove 1,484,977 + -2.1 1,484,978 - -0.2 1,484,978 - -1.0 1,484,978 - +1.2 1,484,978 - +0.2 1,485,091 + -2.7 1,485,092 - -0.4 1,485,092 - +1.2 1,485,092 - -1.1 1,485,136 + -0.1 1,485,136 + -0.8 1,485,136 + +2.2 1,485,136 + -2.2 1,485,136 + +0.6 1,485,136 + -2.5 1,485,137 - +1.2 1,485,137 - -0.4 1,485,137 - -1.3 1,485,137 - -2.4 1,485,192 - -0.7 1,485,196 + -3.0 1,485,196 + -2.9 1,485,196 + -2.1 1,485,196 + +2.2 1,485,196 + -3.2 1,485,197 - ABIE51_RS06905 0.10 +0.4 1,485,197 - ABIE51_RS06905 0.10 -1.2 1,485,197 - ABIE51_RS06905 0.10 +0.4 1,485,197 - ABIE51_RS06905 0.10 -0.7 1,485,197 - ABIE51_RS06905 0.10 -1.6 1,485,199 + ABIE51_RS06905 0.10 +0.6 1,485,199 + ABIE51_RS06905 0.10 +0.6 1,485,199 + ABIE51_RS06905 0.10 -1.9 1,485,199 + ABIE51_RS06905 0.10 -0.8 1,485,200 - ABIE51_RS06905 0.10 -0.7 1,485,200 - ABIE51_RS06905 0.10 +0.6 1,485,200 - ABIE51_RS06905 0.10 +0.6 1,485,200 - ABIE51_RS06905 0.10 -0.6 1,485,200 - ABIE51_RS06905 0.10 +0.4 1,485,366 + ABIE51_RS06905 0.22 -2.0 1,485,463 + ABIE51_RS06905 0.30 -0.2 1,485,464 - ABIE51_RS06905 0.30 -0.2 1,485,775 + ABIE51_RS06905 0.52 -0.6 1,485,775 + ABIE51_RS06905 0.52 -0.5 1,485,775 + ABIE51_RS06905 0.52 -0.5 1,485,775 + ABIE51_RS06905 0.52 +0.9 1,485,776 - ABIE51_RS06905 0.52 -0.1 1,485,795 + ABIE51_RS06905 0.54 -1.1 1,485,795 + ABIE51_RS06905 0.54 +1.8 1,485,795 + ABIE51_RS06905 0.54 +1.5 1,486,219 + ABIE51_RS06905 0.85 -0.6 1,486,220 - ABIE51_RS06905 0.85 +0.0 1,486,220 - ABIE51_RS06905 0.85 -0.0 1,486,250 - ABIE51_RS06905 0.87 -2.0 1,486,345 + +1.2
Or see this region's nucleotide sequence