Experiment: Parafilmed volatile R2A agar plate with no fungus
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ABIE51_RS16660 and ABIE51_RS16665 are separated by 348 nucleotides ABIE51_RS16665 and ABIE51_RS16670 are separated by 146 nucleotides ABIE51_RS16670 and ABIE51_RS16675 are separated by 11 nucleotides ABIE51_RS16675 and ABIE51_RS16680 are separated by 32 nucleotides
ABIE51_RS16660: ABIE51_RS16660 - flagellar basal body P-ring formation chaperone FlgA, at 3,623,712 to 3,624,380
_RS16660
ABIE51_RS16665: ABIE51_RS16665 - chemotaxis protein, at 3,624,729 to 3,625,655
_RS16665
ABIE51_RS16670: ABIE51_RS16670 - flagellar basal body rod protein FlgB, at 3,625,802 to 3,626,161
_RS16670
ABIE51_RS16675: ABIE51_RS16675 - flagellar basal body rod protein FlgC, at 3,626,173 to 3,626,580
_RS16675
ABIE51_RS16680: ABIE51_RS16680 - flagellar hook capping FlgD N-terminal domain-containing protein, at 3,626,613 to 3,627,275
_RS16680
Position (kb)
3624
3625
3626 Strain fitness (log2 ratio)
-1
0
1
2
3 at 3623.762 kb on + strand at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.118 kb on + strand, within ABIE51_RS16660 at 3624.119 kb on - strand, within ABIE51_RS16660 at 3624.119 kb on - strand, within ABIE51_RS16660 at 3624.119 kb on - strand, within ABIE51_RS16660 at 3624.696 kb on - strand at 3625.002 kb on + strand, within ABIE51_RS16665 at 3625.002 kb on + strand, within ABIE51_RS16665 at 3625.003 kb on - strand, within ABIE51_RS16665 at 3625.126 kb on - strand, within ABIE51_RS16665 at 3625.126 kb on - strand, within ABIE51_RS16665 at 3625.423 kb on - strand, within ABIE51_RS16665 at 3626.159 kb on + strand at 3626.159 kb on + strand at 3626.160 kb on - strand at 3626.188 kb on - strand at 3626.408 kb on - strand, within ABIE51_RS16675 at 3626.443 kb on + strand, within ABIE51_RS16675 at 3626.444 kb on - strand, within ABIE51_RS16675 at 3626.444 kb on - strand, within ABIE51_RS16675 at 3626.444 kb on - strand, within ABIE51_RS16675 at 3626.449 kb on + strand, within ABIE51_RS16675 at 3626.450 kb on - strand, within ABIE51_RS16675 at 3626.450 kb on - strand, within ABIE51_RS16675 at 3626.509 kb on + strand, within ABIE51_RS16675 at 3626.509 kb on + strand, within ABIE51_RS16675 at 3626.509 kb on + strand, within ABIE51_RS16675 at 3626.509 kb on + strand, within ABIE51_RS16675 at 3626.510 kb on - strand, within ABIE51_RS16675 at 3626.510 kb on - strand, within ABIE51_RS16675 at 3626.510 kb on - strand, within ABIE51_RS16675 at 3626.510 kb on - strand, within ABIE51_RS16675 at 3626.644 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Parafilmed volatile R2A agar plate with no fungus remove 3,623,762 + -0.2 3,624,118 + ABIE51_RS16660 0.61 -0.1 3,624,118 + ABIE51_RS16660 0.61 -0.2 3,624,118 + ABIE51_RS16660 0.61 +0.3 3,624,118 + ABIE51_RS16660 0.61 +0.0 3,624,118 + ABIE51_RS16660 0.61 +0.3 3,624,118 + ABIE51_RS16660 0.61 +0.4 3,624,118 + ABIE51_RS16660 0.61 +1.1 3,624,118 + ABIE51_RS16660 0.61 -0.3 3,624,118 + ABIE51_RS16660 0.61 +2.8 3,624,119 - ABIE51_RS16660 0.61 +0.2 3,624,119 - ABIE51_RS16660 0.61 -0.3 3,624,119 - ABIE51_RS16660 0.61 +0.3 3,624,696 - +2.0 3,625,002 + ABIE51_RS16665 0.29 +0.8 3,625,002 + ABIE51_RS16665 0.29 +0.6 3,625,003 - ABIE51_RS16665 0.30 -0.0 3,625,126 - ABIE51_RS16665 0.43 -0.2 3,625,126 - ABIE51_RS16665 0.43 -0.1 3,625,423 - ABIE51_RS16665 0.75 -0.4 3,626,159 + +0.3 3,626,159 + -0.6 3,626,160 - +0.6 3,626,188 - -0.6 3,626,408 - ABIE51_RS16675 0.58 +0.3 3,626,443 + ABIE51_RS16675 0.66 -0.3 3,626,444 - ABIE51_RS16675 0.66 -0.1 3,626,444 - ABIE51_RS16675 0.66 +0.2 3,626,444 - ABIE51_RS16675 0.66 -0.6 3,626,449 + ABIE51_RS16675 0.68 +0.0 3,626,450 - ABIE51_RS16675 0.68 -0.3 3,626,450 - ABIE51_RS16675 0.68 -0.3 3,626,509 + ABIE51_RS16675 0.82 -0.1 3,626,509 + ABIE51_RS16675 0.82 +0.2 3,626,509 + ABIE51_RS16675 0.82 -0.5 3,626,509 + ABIE51_RS16675 0.82 +0.2 3,626,510 - ABIE51_RS16675 0.83 -0.0 3,626,510 - ABIE51_RS16675 0.83 +0.0 3,626,510 - ABIE51_RS16675 0.83 +2.0 3,626,510 - ABIE51_RS16675 0.83 -0.7 3,626,644 - +0.2
Or see this region's nucleotide sequence