Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS33285

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS21290 and ECOLIN_RS21295 are separated by 114 nucleotidesECOLIN_RS21295 and ECOLIN_RS33285 are separated by 117 nucleotidesECOLIN_RS33285 and ECOLIN_RS21305 are separated by 20 nucleotides ECOLIN_RS21290: ECOLIN_RS21290 - sugar-phosphatase, at 4,273,598 to 4,274,410 _RS21290 ECOLIN_RS21295: ECOLIN_RS21295 - YidB family protein, at 4,274,525 to 4,274,923 _RS21295 ECOLIN_RS33285: ECOLIN_RS33285 - hypothetical protein, at 4,275,041 to 4,275,142 _RS33285 ECOLIN_RS21305: ECOLIN_RS21305 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 4,275,163 to 4,277,577 _RS21305 Position (kb) 4275 4276Strain fitness (log2 ratio) -1 0 1at 4274.142 kb on + strand, within ECOLIN_RS21290at 4274.209 kb on + strand, within ECOLIN_RS21290at 4274.237 kb on - strand, within ECOLIN_RS21290at 4274.579 kb on - strand, within ECOLIN_RS21295at 4274.937 kb on - strandat 4274.945 kb on - strandat 4274.965 kb on + strandat 4274.966 kb on - strandat 4275.035 kb on + strandat 4275.035 kb on + strandat 4275.164 kb on + strandat 4275.165 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,274,142 + ECOLIN_RS21290 0.67 +0.3
4,274,209 + ECOLIN_RS21290 0.75 -0.1
4,274,237 - ECOLIN_RS21290 0.79 +0.6
4,274,579 - ECOLIN_RS21295 0.14 -0.1
4,274,937 - +0.2
4,274,945 - -0.1
4,274,965 + +0.1
4,274,966 - +0.3
4,275,035 + +0.0
4,275,035 + +0.2
4,275,164 + +0.2
4,275,165 - +0.2

Or see this region's nucleotide sequence