Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS21305

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS21290 and ECOLIN_RS21295 are separated by 114 nucleotidesECOLIN_RS21295 and ECOLIN_RS33285 are separated by 117 nucleotidesECOLIN_RS33285 and ECOLIN_RS21305 are separated by 20 nucleotidesECOLIN_RS21305 and ECOLIN_RS21310 are separated by 28 nucleotides ECOLIN_RS21290: ECOLIN_RS21290 - sugar-phosphatase, at 4,273,598 to 4,274,410 _RS21290 ECOLIN_RS21295: ECOLIN_RS21295 - YidB family protein, at 4,274,525 to 4,274,923 _RS21295 ECOLIN_RS33285: ECOLIN_RS33285 - hypothetical protein, at 4,275,041 to 4,275,142 _RS33285 ECOLIN_RS21305: ECOLIN_RS21305 - DNA topoisomerase (ATP-hydrolyzing) subunit B, at 4,275,163 to 4,277,577 _RS21305 ECOLIN_RS21310: ECOLIN_RS21310 - DNA replication/repair protein RecF, at 4,277,606 to 4,278,679 _RS21310 Position (kb) 4275 4276 4277 4278Strain fitness (log2 ratio) -1 0 1at 4274.209 kb on + strand, within ECOLIN_RS21290at 4274.237 kb on - strand, within ECOLIN_RS21290at 4274.579 kb on - strand, within ECOLIN_RS21295at 4274.937 kb on - strandat 4274.945 kb on - strandat 4274.965 kb on + strandat 4274.966 kb on - strandat 4275.035 kb on + strandat 4275.035 kb on + strandat 4275.164 kb on + strandat 4275.165 kb on - strandat 4277.597 kb on - strandat 4277.666 kb on + strandat 4277.667 kb on - strandat 4277.667 kb on - strandat 4277.667 kb on - strandat 4277.667 kb on - strandat 4277.778 kb on - strand, within ECOLIN_RS21310at 4277.784 kb on + strand, within ECOLIN_RS21310at 4277.838 kb on - strand, within ECOLIN_RS21310at 4277.857 kb on + strand, within ECOLIN_RS21310at 4277.878 kb on + strand, within ECOLIN_RS21310at 4277.878 kb on + strand, within ECOLIN_RS21310at 4277.923 kb on + strand, within ECOLIN_RS21310at 4277.944 kb on - strand, within ECOLIN_RS21310at 4277.949 kb on - strand, within ECOLIN_RS21310at 4278.064 kb on + strand, within ECOLIN_RS21310at 4278.095 kb on - strand, within ECOLIN_RS21310at 4278.096 kb on + strand, within ECOLIN_RS21310at 4278.159 kb on - strand, within ECOLIN_RS21310at 4278.549 kb on + strand, within ECOLIN_RS21310at 4278.549 kb on + strand, within ECOLIN_RS21310at 4278.550 kb on - strand, within ECOLIN_RS21310at 4278.550 kb on - strand, within ECOLIN_RS21310at 4278.550 kb on - strand, within ECOLIN_RS21310

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,274,209 + ECOLIN_RS21290 0.75 -0.1
4,274,237 - ECOLIN_RS21290 0.79 +0.6
4,274,579 - ECOLIN_RS21295 0.14 -0.1
4,274,937 - +0.2
4,274,945 - -0.1
4,274,965 + +0.1
4,274,966 - +0.3
4,275,035 + +0.0
4,275,035 + +0.2
4,275,164 + +0.2
4,275,165 - +0.2
4,277,597 - +0.2
4,277,666 + +0.1
4,277,667 - -0.1
4,277,667 - -0.1
4,277,667 - -0.1
4,277,667 - -0.1
4,277,778 - ECOLIN_RS21310 0.16 -0.3
4,277,784 + ECOLIN_RS21310 0.17 -0.3
4,277,838 - ECOLIN_RS21310 0.22 -0.3
4,277,857 + ECOLIN_RS21310 0.23 -0.5
4,277,878 + ECOLIN_RS21310 0.25 -0.6
4,277,878 + ECOLIN_RS21310 0.25 -0.5
4,277,923 + ECOLIN_RS21310 0.30 -0.6
4,277,944 - ECOLIN_RS21310 0.31 +0.0
4,277,949 - ECOLIN_RS21310 0.32 +0.1
4,278,064 + ECOLIN_RS21310 0.43 -0.4
4,278,095 - ECOLIN_RS21310 0.46 -0.1
4,278,096 + ECOLIN_RS21310 0.46 +0.2
4,278,159 - ECOLIN_RS21310 0.51 -0.4
4,278,549 + ECOLIN_RS21310 0.88 -0.2
4,278,549 + ECOLIN_RS21310 0.88 -0.8
4,278,550 - ECOLIN_RS21310 0.88 -0.1
4,278,550 - ECOLIN_RS21310 0.88 +0.1
4,278,550 - ECOLIN_RS21310 0.88 +0.1

Or see this region's nucleotide sequence