Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20590

Experiment: Sodium-DL-Lactate

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20580 and ECOLIN_RS20585 are separated by 47 nucleotidesECOLIN_RS20585 and ECOLIN_RS20590 are separated by 54 nucleotidesECOLIN_RS20590 and ECOLIN_RS20595 are separated by 79 nucleotides ECOLIN_RS20580: ECOLIN_RS20580 - quinone-dependent L-lactate dehydrogenase, at 4,147,493 to 4,148,683 _RS20580 ECOLIN_RS20585: ECOLIN_RS20585 - tRNA (uridine(34)/cytosine(34)/5- carboxymethylaminomethyluridine(34)-2'-O)- methyltransferase TrmL, at 4,148,731 to 4,149,204 _RS20585 ECOLIN_RS20590: ECOLIN_RS20590 - serine O-acetyltransferase, at 4,149,259 to 4,150,080 _RS20590 ECOLIN_RS20595: ECOLIN_RS20595 - NAD(P)H-dependent glycerol-3-phosphate dehydrogenase, at 4,150,160 to 4,151,179 _RS20595 Position (kb) 4149 4150 4151Strain fitness (log2 ratio) -6 -5 -4 -3 -2 -1 0 1at 4148.331 kb on + strand, within ECOLIN_RS20580at 4148.341 kb on - strand, within ECOLIN_RS20580at 4148.419 kb on - strand, within ECOLIN_RS20580at 4148.443 kb on + strand, within ECOLIN_RS20580at 4148.443 kb on + strand, within ECOLIN_RS20580at 4148.528 kb on - strand, within ECOLIN_RS20580at 4148.528 kb on - strand, within ECOLIN_RS20580at 4148.696 kb on + strandat 4148.730 kb on + strandat 4148.730 kb on + strandat 4148.735 kb on + strandat 4148.735 kb on + strandat 4148.745 kb on - strandat 4148.772 kb on + strandat 4148.773 kb on - strandat 4148.976 kb on + strand, within ECOLIN_RS20585at 4148.996 kb on + strand, within ECOLIN_RS20585at 4149.154 kb on + strand, within ECOLIN_RS20585at 4149.275 kb on + strandat 4149.286 kb on - strandat 4149.315 kb on + strandat 4149.559 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.649 kb on + strand, within ECOLIN_RS20590at 4149.650 kb on - strand, within ECOLIN_RS20590at 4149.650 kb on - strand, within ECOLIN_RS20590at 4149.770 kb on + strand, within ECOLIN_RS20590at 4149.772 kb on + strand, within ECOLIN_RS20590at 4149.789 kb on - strand, within ECOLIN_RS20590at 4149.789 kb on - strand, within ECOLIN_RS20590at 4149.789 kb on - strand, within ECOLIN_RS20590at 4149.792 kb on + strand, within ECOLIN_RS20590at 4149.828 kb on + strand, within ECOLIN_RS20590at 4149.909 kb on + strand, within ECOLIN_RS20590at 4149.910 kb on - strand, within ECOLIN_RS20590at 4149.941 kb on + strand, within ECOLIN_RS20590at 4149.941 kb on + strand, within ECOLIN_RS20590at 4149.942 kb on - strand, within ECOLIN_RS20590at 4150.041 kb on + strandat 4150.044 kb on + strandat 4150.085 kb on + strandat 4150.103 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Sodium-DL-Lactate
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4,148,331 + ECOLIN_RS20580 0.70 -0.3
4,148,341 - ECOLIN_RS20580 0.71 -0.3
4,148,419 - ECOLIN_RS20580 0.78 -1.7
4,148,443 + ECOLIN_RS20580 0.80 -0.3
4,148,443 + ECOLIN_RS20580 0.80 -0.9
4,148,528 - ECOLIN_RS20580 0.87 -1.0
4,148,528 - ECOLIN_RS20580 0.87 -0.3
4,148,696 + +0.9
4,148,730 + +0.0
4,148,730 + +0.0
4,148,735 + -0.2
4,148,735 + -0.1
4,148,745 - +0.8
4,148,772 + +0.1
4,148,773 - +0.4
4,148,976 + ECOLIN_RS20585 0.52 +0.2
4,148,996 + ECOLIN_RS20585 0.56 -0.0
4,149,154 + ECOLIN_RS20585 0.89 -0.0
4,149,275 + +0.2
4,149,286 - -0.5
4,149,315 + -4.1
4,149,559 + ECOLIN_RS20590 0.36 -6.1
4,149,649 + ECOLIN_RS20590 0.47 -4.9
4,149,649 + ECOLIN_RS20590 0.47 -3.0
4,149,649 + ECOLIN_RS20590 0.47 -3.9
4,149,649 + ECOLIN_RS20590 0.47 -4.7
4,149,650 - ECOLIN_RS20590 0.48 -2.0
4,149,650 - ECOLIN_RS20590 0.48 -4.5
4,149,770 + ECOLIN_RS20590 0.62 -5.0
4,149,772 + ECOLIN_RS20590 0.62 -5.3
4,149,789 - ECOLIN_RS20590 0.64 -2.9
4,149,789 - ECOLIN_RS20590 0.64 -5.4
4,149,789 - ECOLIN_RS20590 0.64 -4.0
4,149,792 + ECOLIN_RS20590 0.65 -6.0
4,149,828 + ECOLIN_RS20590 0.69 -1.8
4,149,909 + ECOLIN_RS20590 0.79 -4.6
4,149,910 - ECOLIN_RS20590 0.79 -2.9
4,149,941 + ECOLIN_RS20590 0.83 -3.8
4,149,941 + ECOLIN_RS20590 0.83 -2.3
4,149,942 - ECOLIN_RS20590 0.83 -3.4
4,150,041 + -4.2
4,150,044 + -5.0
4,150,085 + -3.8
4,150,103 - +0.1

Or see this region's nucleotide sequence