Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS16045

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16040 and ECOLIN_RS16045 are separated by 2 nucleotidesECOLIN_RS16045 and ECOLIN_RS16050 are separated by 50 nucleotidesECOLIN_RS16050 and ECOLIN_RS16055 overlap by 4 nucleotides ECOLIN_RS16040: ECOLIN_RS16040 - putative selenate reductase subunit YgfK, at 3,248,615 to 3,251,713 _RS16040 ECOLIN_RS16045: ECOLIN_RS16045 - putative aminohydrolase SsnA, at 3,251,716 to 3,253,044 _RS16045 ECOLIN_RS16050: ECOLIN_RS16050 - molybdopterin-dependent oxidoreductase FAD-binding subunit, at 3,253,095 to 3,253,874 _RS16050 ECOLIN_RS16055: ECOLIN_RS16055 - molybdopterin-dependent oxidoreductase Mo/Fe-S-binding subunit, at 3,253,871 to 3,256,741 _RS16055 Position (kb) 3251 3252 3253 3254Strain fitness (log2 ratio) -2 -1 0 1at 3250.947 kb on + strand, within ECOLIN_RS16040at 3251.191 kb on + strand, within ECOLIN_RS16040at 3251.192 kb on - strand, within ECOLIN_RS16040at 3251.318 kb on - strand, within ECOLIN_RS16040at 3251.321 kb on + strand, within ECOLIN_RS16040at 3251.647 kb on + strandat 3251.647 kb on + strandat 3251.648 kb on - strandat 3251.648 kb on - strandat 3251.687 kb on + strandat 3251.711 kb on + strandat 3251.712 kb on - strandat 3251.750 kb on + strandat 3251.750 kb on + strandat 3252.106 kb on + strand, within ECOLIN_RS16045at 3252.514 kb on + strand, within ECOLIN_RS16045at 3252.514 kb on + strand, within ECOLIN_RS16045at 3252.985 kb on + strandat 3253.014 kb on + strandat 3253.014 kb on + strandat 3253.015 kb on - strandat 3253.042 kb on + strandat 3253.042 kb on + strandat 3253.042 kb on + strandat 3253.046 kb on + strandat 3253.046 kb on + strandat 3253.046 kb on + strandat 3253.334 kb on + strand, within ECOLIN_RS16050at 3253.513 kb on - strand, within ECOLIN_RS16050at 3253.557 kb on - strand, within ECOLIN_RS16050at 3253.797 kb on + strandat 3253.806 kb on + strandat 3253.808 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,250,947 + ECOLIN_RS16040 0.75 +0.2
3,251,191 + ECOLIN_RS16040 0.83 -0.3
3,251,192 - ECOLIN_RS16040 0.83 -0.3
3,251,318 - ECOLIN_RS16040 0.87 -0.6
3,251,321 + ECOLIN_RS16040 0.87 +0.3
3,251,647 + +0.0
3,251,647 + -0.2
3,251,648 - +0.1
3,251,648 - +0.2
3,251,687 + -0.6
3,251,711 + -0.4
3,251,712 - +0.3
3,251,750 + -1.6
3,251,750 + -2.4
3,252,106 + ECOLIN_RS16045 0.29 -0.4
3,252,514 + ECOLIN_RS16045 0.60 +1.0
3,252,514 + ECOLIN_RS16045 0.60 +0.0
3,252,985 + +0.0
3,253,014 + +0.5
3,253,014 + +0.2
3,253,015 - +0.1
3,253,042 + +0.7
3,253,042 + +0.3
3,253,042 + +0.2
3,253,046 + +0.7
3,253,046 + +0.1
3,253,046 + -0.3
3,253,334 + ECOLIN_RS16050 0.31 -0.4
3,253,513 - ECOLIN_RS16050 0.54 -0.5
3,253,557 - ECOLIN_RS16050 0.59 -0.5
3,253,797 + -0.3
3,253,806 + +0.6
3,253,808 + -1.0

Or see this region's nucleotide sequence