Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20790

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20780 and ECOLIN_RS20785 are separated by 54 nucleotidesECOLIN_RS20785 and ECOLIN_RS20790 are separated by 18 nucleotidesECOLIN_RS20790 and ECOLIN_RS20795 are separated by 6 nucleotidesECOLIN_RS20795 and ECOLIN_RS20800 are separated by 5 nucleotides ECOLIN_RS20780: ECOLIN_RS20780 - guanylate kinase, at 4,185,763 to 4,186,386 _RS20780 ECOLIN_RS20785: ECOLIN_RS20785 - DNA-directed RNA polymerase subunit omega, at 4,186,441 to 4,186,716 _RS20785 ECOLIN_RS20790: ECOLIN_RS20790 - bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase, at 4,186,735 to 4,188,843 _RS20790 ECOLIN_RS20795: ECOLIN_RS20795 - tRNA (guanosine(18)-2'-O)-methyltransferase TrmH, at 4,188,850 to 4,189,539 _RS20795 ECOLIN_RS20800: ECOLIN_RS20800 - ATP-dependent DNA helicase RecG, at 4,189,545 to 4,191,626 _RS20800 Position (kb) 4186 4187 4188 4189Strain fitness (log2 ratio) -3 -2 -1 0 1at 4185.749 kb on + strandat 4187.900 kb on - strand, within ECOLIN_RS20790at 4187.900 kb on - strand, within ECOLIN_RS20790at 4188.694 kb on - strandat 4188.734 kb on - strandat 4188.819 kb on + strandat 4188.842 kb on - strandat 4189.047 kb on + strand, within ECOLIN_RS20795at 4189.051 kb on - strand, within ECOLIN_RS20795at 4189.361 kb on - strand, within ECOLIN_RS20795at 4189.538 kb on - strandat 4189.562 kb on - strandat 4189.608 kb on - strandat 4189.657 kb on + strandat 4189.712 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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4,185,749 + +0.6
4,187,900 - ECOLIN_RS20790 0.55 -0.2
4,187,900 - ECOLIN_RS20790 0.55 +1.0
4,188,694 - -0.5
4,188,734 - -0.3
4,188,819 + -1.3
4,188,842 - +1.4
4,189,047 + ECOLIN_RS20795 0.29 -2.2
4,189,051 - ECOLIN_RS20795 0.29 -3.7
4,189,361 - ECOLIN_RS20795 0.74 -0.6
4,189,538 - -3.1
4,189,562 - +1.5
4,189,608 - -1.2
4,189,657 + -1.3
4,189,712 - -1.5

Or see this region's nucleotide sequence