Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS19350

Experiment: L-Rhamnose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS19340 and ECOLIN_RS19345 overlap by 8 nucleotidesECOLIN_RS19345 and ECOLIN_RS19350 are separated by 17 nucleotidesECOLIN_RS19350 and ECOLIN_RS19355 are separated by 106 nucleotides ECOLIN_RS19340: ECOLIN_RS19340 - phosphoglycolate phosphatase, at 3,869,070 to 3,869,828 _RS19340 ECOLIN_RS19345: ECOLIN_RS19345 - ribulose-phosphate 3-epimerase, at 3,869,821 to 3,870,498 _RS19345 ECOLIN_RS19350: ECOLIN_RS19350 - adenine-specific DNA-methyltransferase, at 3,870,516 to 3,871,352 _RS19350 ECOLIN_RS19355: ECOLIN_RS19355 - cell division protein DamX, at 3,871,459 to 3,872,745 _RS19355 Position (kb) 3870 3871 3872Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 3869.524 kb on + strand, within ECOLIN_RS19340at 3869.552 kb on + strand, within ECOLIN_RS19340at 3869.557 kb on - strand, within ECOLIN_RS19340at 3870.298 kb on + strand, within ECOLIN_RS19345at 3870.298 kb on + strand, within ECOLIN_RS19345at 3870.299 kb on - strand, within ECOLIN_RS19345at 3870.327 kb on + strand, within ECOLIN_RS19345at 3870.328 kb on - strand, within ECOLIN_RS19345at 3870.328 kb on - strand, within ECOLIN_RS19345at 3870.374 kb on + strand, within ECOLIN_RS19345at 3870.375 kb on - strand, within ECOLIN_RS19345at 3870.375 kb on - strand, within ECOLIN_RS19345at 3870.375 kb on - strand, within ECOLIN_RS19345at 3870.488 kb on + strandat 3870.489 kb on - strandat 3870.518 kb on - strandat 3870.601 kb on - strand, within ECOLIN_RS19350at 3870.602 kb on + strand, within ECOLIN_RS19350at 3870.602 kb on + strand, within ECOLIN_RS19350at 3870.602 kb on + strand, within ECOLIN_RS19350at 3871.132 kb on - strand, within ECOLIN_RS19350at 3871.202 kb on + strand, within ECOLIN_RS19350at 3871.205 kb on - strand, within ECOLIN_RS19350at 3871.293 kb on - strandat 3871.364 kb on - strandat 3871.364 kb on - strandat 3871.364 kb on - strandat 3871.534 kb on - strandat 3871.569 kb on - strandat 3871.589 kb on + strand, within ECOLIN_RS19355at 3871.589 kb on + strand, within ECOLIN_RS19355at 3871.620 kb on - strand, within ECOLIN_RS19355at 3871.741 kb on - strand, within ECOLIN_RS19355at 3872.322 kb on + strand, within ECOLIN_RS19355

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Rhamnose
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3,869,524 + ECOLIN_RS19340 0.60 +0.1
3,869,552 + ECOLIN_RS19340 0.64 -1.1
3,869,557 - ECOLIN_RS19340 0.64 +0.3
3,870,298 + ECOLIN_RS19345 0.70 -0.9
3,870,298 + ECOLIN_RS19345 0.70 -1.4
3,870,299 - ECOLIN_RS19345 0.71 -1.2
3,870,327 + ECOLIN_RS19345 0.75 -1.4
3,870,328 - ECOLIN_RS19345 0.75 +0.3
3,870,328 - ECOLIN_RS19345 0.75 -0.4
3,870,374 + ECOLIN_RS19345 0.82 -0.6
3,870,375 - ECOLIN_RS19345 0.82 -0.8
3,870,375 - ECOLIN_RS19345 0.82 -0.9
3,870,375 - ECOLIN_RS19345 0.82 -1.4
3,870,488 + +2.1
3,870,489 - +1.3
3,870,518 - +0.7
3,870,601 - ECOLIN_RS19350 0.10 -3.2
3,870,602 + ECOLIN_RS19350 0.10 -1.4
3,870,602 + ECOLIN_RS19350 0.10 -1.9
3,870,602 + ECOLIN_RS19350 0.10 -3.5
3,871,132 - ECOLIN_RS19350 0.74 -0.2
3,871,202 + ECOLIN_RS19350 0.82 +2.5
3,871,205 - ECOLIN_RS19350 0.82 -2.0
3,871,293 - +0.5
3,871,364 - -0.7
3,871,364 - +0.4
3,871,364 - -0.6
3,871,534 - -1.9
3,871,569 - +0.2
3,871,589 + ECOLIN_RS19355 0.10 -0.1
3,871,589 + ECOLIN_RS19355 0.10 -0.7
3,871,620 - ECOLIN_RS19355 0.13 -0.3
3,871,741 - ECOLIN_RS19355 0.22 +1.5
3,872,322 + ECOLIN_RS19355 0.67 -0.9

Or see this region's nucleotide sequence