Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS00365

Experiment: L-Arabinose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS00355 and ECOLIN_RS00360 are separated by 113 nucleotidesECOLIN_RS00360 and ECOLIN_RS00365 overlap by 17 nucleotidesECOLIN_RS00365 and ECOLIN_RS00370 overlap by 25 nucleotides ECOLIN_RS00355: ECOLIN_RS00355 - DedA family protein, at 74,331 to 75,095 _RS00355 ECOLIN_RS00360: ECOLIN_RS00360 - thiamine ABC transporter ATP-binding protein ThiQ, at 75,209 to 75,907 _RS00360 ECOLIN_RS00365: ECOLIN_RS00365 - thiamine/thiamine pyrophosphate ABC transporter permease ThiP, at 75,891 to 77,501 _RS00365 ECOLIN_RS00370: ECOLIN_RS00370 - thiamine ABC transporter substrate binding subunit, at 77,477 to 78,460 _RS00370 Position (kb) 75 76 77 78Strain fitness (log2 ratio) -1 0 1 2 3at 74.962 kb on - strand, within ECOLIN_RS00355at 75.060 kb on + strandat 75.060 kb on + strandat 75.169 kb on - strandat 75.208 kb on - strandat 75.211 kb on - strandat 75.211 kb on - strandat 75.603 kb on - strand, within ECOLIN_RS00360at 75.890 kb on - strandat 76.152 kb on + strand, within ECOLIN_RS00365at 76.153 kb on - strand, within ECOLIN_RS00365at 76.474 kb on - strand, within ECOLIN_RS00365at 76.643 kb on + strand, within ECOLIN_RS00365at 76.644 kb on - strand, within ECOLIN_RS00365at 76.810 kb on + strand, within ECOLIN_RS00365at 76.896 kb on + strand, within ECOLIN_RS00365at 76.896 kb on + strand, within ECOLIN_RS00365at 77.028 kb on + strand, within ECOLIN_RS00365at 77.769 kb on + strand, within ECOLIN_RS00370at 77.774 kb on - strand, within ECOLIN_RS00370at 77.818 kb on - strand, within ECOLIN_RS00370at 77.978 kb on - strand, within ECOLIN_RS00370at 78.381 kb on + strandat 78.381 kb on + strandat 78.382 kb on - strandat 78.382 kb on - strandat 78.385 kb on + strandat 78.386 kb on - strandat 78.387 kb on + strandat 78.388 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction L-Arabinose
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74,962 - ECOLIN_RS00355 0.82 -0.0
75,060 + -0.1
75,060 + +0.1
75,169 - +1.3
75,208 - +0.0
75,211 - -1.3
75,211 - +0.5
75,603 - ECOLIN_RS00360 0.56 +0.2
75,890 - +0.9
76,152 + ECOLIN_RS00365 0.16 +0.2
76,153 - ECOLIN_RS00365 0.16 -0.5
76,474 - ECOLIN_RS00365 0.36 -0.3
76,643 + ECOLIN_RS00365 0.47 +1.8
76,644 - ECOLIN_RS00365 0.47 +0.3
76,810 + ECOLIN_RS00365 0.57 -0.4
76,896 + ECOLIN_RS00365 0.62 +1.2
76,896 + ECOLIN_RS00365 0.62 +0.4
77,028 + ECOLIN_RS00365 0.71 +0.3
77,769 + ECOLIN_RS00370 0.30 +0.2
77,774 - ECOLIN_RS00370 0.30 -1.2
77,818 - ECOLIN_RS00370 0.35 -0.1
77,978 - ECOLIN_RS00370 0.51 -0.0
78,381 + +0.6
78,381 + +3.0
78,382 - +0.7
78,382 - +0.3
78,385 + -0.2
78,386 - +0.1
78,387 + +0.1
78,388 - +0.1

Or see this region's nucleotide sequence