Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS22850

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS22845 and ECOLIN_RS22850 are separated by 7 nucleotidesECOLIN_RS22850 and ECOLIN_RS22855 are separated by 60 nucleotides ECOLIN_RS22845: ECOLIN_RS22845 - N-acetyl-gamma-glutamyl-phosphate reductase, at 4,589,475 to 4,590,479 _RS22845 ECOLIN_RS22850: ECOLIN_RS22850 - acetylglutamate kinase, at 4,590,487 to 4,591,263 _RS22850 ECOLIN_RS22855: ECOLIN_RS22855 - argininosuccinate lyase, at 4,591,324 to 4,592,697 _RS22855 Position (kb) 4590 4591 4592Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 4589.524 kb on + strandat 4589.532 kb on + strandat 4589.532 kb on + strandat 4589.536 kb on + strandat 4589.536 kb on + strandat 4589.561 kb on - strandat 4589.606 kb on - strand, within ECOLIN_RS22845at 4589.894 kb on + strand, within ECOLIN_RS22845at 4589.897 kb on + strand, within ECOLIN_RS22845at 4589.898 kb on - strand, within ECOLIN_RS22845at 4590.401 kb on - strandat 4590.473 kb on + strandat 4590.501 kb on - strandat 4590.704 kb on + strand, within ECOLIN_RS22850at 4590.705 kb on - strand, within ECOLIN_RS22850at 4590.712 kb on - strand, within ECOLIN_RS22850at 4590.741 kb on + strand, within ECOLIN_RS22850at 4590.785 kb on + strand, within ECOLIN_RS22850at 4590.883 kb on - strand, within ECOLIN_RS22850at 4591.063 kb on - strand, within ECOLIN_RS22850at 4591.063 kb on - strand, within ECOLIN_RS22850at 4591.063 kb on - strand, within ECOLIN_RS22850at 4591.063 kb on - strand, within ECOLIN_RS22850at 4591.113 kb on + strand, within ECOLIN_RS22850at 4591.256 kb on + strandat 4591.423 kb on - strandat 4591.579 kb on - strand, within ECOLIN_RS22855at 4591.727 kb on + strand, within ECOLIN_RS22855at 4591.727 kb on + strand, within ECOLIN_RS22855at 4591.728 kb on - strand, within ECOLIN_RS22855at 4591.728 kb on - strand, within ECOLIN_RS22855at 4591.884 kb on + strand, within ECOLIN_RS22855

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,589,524 + -1.2
4,589,532 + -1.4
4,589,532 + +0.1
4,589,536 + +0.4
4,589,536 + -1.9
4,589,561 - -1.3
4,589,606 - ECOLIN_RS22845 0.13 -2.4
4,589,894 + ECOLIN_RS22845 0.42 +0.4
4,589,897 + ECOLIN_RS22845 0.42 -0.5
4,589,898 - ECOLIN_RS22845 0.42 -1.9
4,590,401 - -1.7
4,590,473 + -0.0
4,590,501 - -2.9
4,590,704 + ECOLIN_RS22850 0.28 -1.9
4,590,705 - ECOLIN_RS22850 0.28 -3.5
4,590,712 - ECOLIN_RS22850 0.29 -3.8
4,590,741 + ECOLIN_RS22850 0.33 -0.8
4,590,785 + ECOLIN_RS22850 0.38 -0.4
4,590,883 - ECOLIN_RS22850 0.51 -3.2
4,591,063 - ECOLIN_RS22850 0.74 -2.8
4,591,063 - ECOLIN_RS22850 0.74 -2.9
4,591,063 - ECOLIN_RS22850 0.74 -2.7
4,591,063 - ECOLIN_RS22850 0.74 -1.8
4,591,113 + ECOLIN_RS22850 0.81 -1.6
4,591,256 + +0.3
4,591,423 - -3.3
4,591,579 - ECOLIN_RS22855 0.19 -4.4
4,591,727 + ECOLIN_RS22855 0.29 -4.9
4,591,727 + ECOLIN_RS22855 0.29 -2.8
4,591,728 - ECOLIN_RS22855 0.29 -5.2
4,591,728 - ECOLIN_RS22855 0.29 -6.8
4,591,884 + ECOLIN_RS22855 0.41 -2.6

Or see this region's nucleotide sequence