Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS20445

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS20440 and ECOLIN_RS20445 are separated by 200 nucleotidesECOLIN_RS20445 and ECOLIN_RS20450 are separated by 11 nucleotidesECOLIN_RS20450 and ECOLIN_RS20455 are separated by 22 nucleotides ECOLIN_RS20440: ECOLIN_RS20440 - IclR family transcriptional regulator YiaJ, at 4,112,563 to 4,113,411 _RS20440 ECOLIN_RS20445: ECOLIN_RS20445 - 3-dehydro-L-gulonate 2-dehydrogenase, at 4,113,612 to 4,114,610 _RS20445 ECOLIN_RS20450: ECOLIN_RS20450 - YhcH/YjgK/YiaL family protein, at 4,114,622 to 4,115,086 _RS20450 ECOLIN_RS20455: ECOLIN_RS20455 - DUF4862 family protein, at 4,115,109 to 4,116,029 _RS20455 Position (kb) 4113 4114 4115Strain fitness (log2 ratio) -1 0 1 2at 4112.616 kb on + strandat 4112.616 kb on + strandat 4112.616 kb on + strandat 4112.949 kb on - strand, within ECOLIN_RS20440at 4112.968 kb on + strand, within ECOLIN_RS20440at 4112.970 kb on + strand, within ECOLIN_RS20440at 4112.973 kb on - strand, within ECOLIN_RS20440at 4112.973 kb on - strand, within ECOLIN_RS20440at 4112.973 kb on - strand, within ECOLIN_RS20440at 4113.012 kb on + strand, within ECOLIN_RS20440at 4113.013 kb on - strand, within ECOLIN_RS20440at 4113.013 kb on - strand, within ECOLIN_RS20440at 4113.055 kb on - strand, within ECOLIN_RS20440at 4113.058 kb on - strand, within ECOLIN_RS20440at 4113.150 kb on + strand, within ECOLIN_RS20440at 4113.151 kb on - strand, within ECOLIN_RS20440at 4113.635 kb on - strandat 4113.635 kb on - strandat 4113.978 kb on + strand, within ECOLIN_RS20445at 4114.005 kb on + strand, within ECOLIN_RS20445at 4114.006 kb on - strand, within ECOLIN_RS20445at 4114.159 kb on + strand, within ECOLIN_RS20445at 4114.160 kb on - strand, within ECOLIN_RS20445at 4114.195 kb on + strand, within ECOLIN_RS20445at 4114.314 kb on - strand, within ECOLIN_RS20445at 4114.470 kb on + strand, within ECOLIN_RS20445at 4114.470 kb on + strand, within ECOLIN_RS20445at 4114.471 kb on - strand, within ECOLIN_RS20445at 4114.471 kb on - strand, within ECOLIN_RS20445at 4114.532 kb on + strandat 4114.533 kb on - strandat 4114.565 kb on + strandat 4114.616 kb on + strandat 4114.823 kb on + strand, within ECOLIN_RS20450at 4114.912 kb on - strand, within ECOLIN_RS20450at 4114.978 kb on + strand, within ECOLIN_RS20450at 4114.978 kb on + strand, within ECOLIN_RS20450at 4115.008 kb on - strand, within ECOLIN_RS20450at 4115.024 kb on - strand, within ECOLIN_RS20450at 4115.132 kb on + strandat 4115.133 kb on - strandat 4115.133 kb on - strandat 4115.210 kb on - strand, within ECOLIN_RS20455at 4115.294 kb on + strand, within ECOLIN_RS20455at 4115.416 kb on - strand, within ECOLIN_RS20455at 4115.436 kb on - strand, within ECOLIN_RS20455

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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4,112,616 + -1.2
4,112,616 + -1.3
4,112,616 + +0.5
4,112,949 - ECOLIN_RS20440 0.45 -0.6
4,112,968 + ECOLIN_RS20440 0.48 +1.1
4,112,970 + ECOLIN_RS20440 0.48 -0.3
4,112,973 - ECOLIN_RS20440 0.48 -0.4
4,112,973 - ECOLIN_RS20440 0.48 -0.3
4,112,973 - ECOLIN_RS20440 0.48 -0.2
4,113,012 + ECOLIN_RS20440 0.53 -0.1
4,113,013 - ECOLIN_RS20440 0.53 -0.7
4,113,013 - ECOLIN_RS20440 0.53 -0.9
4,113,055 - ECOLIN_RS20440 0.58 -0.2
4,113,058 - ECOLIN_RS20440 0.58 -0.7
4,113,150 + ECOLIN_RS20440 0.69 +0.4
4,113,151 - ECOLIN_RS20440 0.69 -0.4
4,113,635 - -0.1
4,113,635 - -0.1
4,113,978 + ECOLIN_RS20445 0.37 +0.3
4,114,005 + ECOLIN_RS20445 0.39 -0.6
4,114,006 - ECOLIN_RS20445 0.39 -0.4
4,114,159 + ECOLIN_RS20445 0.55 +0.3
4,114,160 - ECOLIN_RS20445 0.55 -0.3
4,114,195 + ECOLIN_RS20445 0.58 +2.1
4,114,314 - ECOLIN_RS20445 0.70 +0.0
4,114,470 + ECOLIN_RS20445 0.86 +0.6
4,114,470 + ECOLIN_RS20445 0.86 -0.3
4,114,471 - ECOLIN_RS20445 0.86 -0.2
4,114,471 - ECOLIN_RS20445 0.86 +0.2
4,114,532 + +0.5
4,114,533 - -0.1
4,114,565 + -0.3
4,114,616 + -1.2
4,114,823 + ECOLIN_RS20450 0.43 -0.5
4,114,912 - ECOLIN_RS20450 0.62 -0.6
4,114,978 + ECOLIN_RS20450 0.77 +1.3
4,114,978 + ECOLIN_RS20450 0.77 +0.1
4,115,008 - ECOLIN_RS20450 0.83 -0.4
4,115,024 - ECOLIN_RS20450 0.86 +1.4
4,115,132 + +0.5
4,115,133 - +0.3
4,115,133 - +0.1
4,115,210 - ECOLIN_RS20455 0.11 +0.2
4,115,294 + ECOLIN_RS20455 0.20 +0.2
4,115,416 - ECOLIN_RS20455 0.33 -0.6
4,115,436 - ECOLIN_RS20455 0.36 +0.0

Or see this region's nucleotide sequence