Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS17685

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS17675 and ECOLIN_RS17680 overlap by 4 nucleotidesECOLIN_RS17680 and ECOLIN_RS17685 are separated by 47 nucleotidesECOLIN_RS17685 and ECOLIN_RS17690 are separated by 42 nucleotides ECOLIN_RS17675: ECOLIN_RS17675 - L(+)-tartrate dehydratase subunit alpha, at 3,560,803 to 3,561,714 _RS17675 ECOLIN_RS17680: ECOLIN_RS17680 - L(+)-tartrate dehydratase subunit beta, at 3,561,711 to 3,562,316 _RS17680 ECOLIN_RS17685: ECOLIN_RS17685 - L-tartrate/succinate antiporter, at 3,562,364 to 3,563,827 _RS17685 ECOLIN_RS17690: ECOLIN_RS17690 - tRNA (adenosine(37)-N6)-threonylcarbamoyltransferase complex transferase subunit TsaD, at 3,563,870 to 3,564,883 _RS17690 Position (kb) 3562 3563 3564Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 3561.497 kb on + strand, within ECOLIN_RS17675at 3561.780 kb on + strand, within ECOLIN_RS17680at 3561.780 kb on + strand, within ECOLIN_RS17680at 3562.132 kb on + strand, within ECOLIN_RS17680at 3562.132 kb on + strand, within ECOLIN_RS17680at 3562.208 kb on + strand, within ECOLIN_RS17680at 3562.209 kb on - strand, within ECOLIN_RS17680at 3562.211 kb on - strand, within ECOLIN_RS17680at 3562.211 kb on - strand, within ECOLIN_RS17680at 3562.279 kb on + strandat 3562.280 kb on - strandat 3562.315 kb on - strandat 3562.358 kb on - strandat 3562.429 kb on - strandat 3562.429 kb on - strandat 3562.429 kb on - strandat 3562.459 kb on + strandat 3562.459 kb on + strandat 3562.459 kb on + strandat 3562.459 kb on + strandat 3562.549 kb on - strand, within ECOLIN_RS17685at 3562.549 kb on - strand, within ECOLIN_RS17685at 3562.615 kb on + strand, within ECOLIN_RS17685at 3562.615 kb on + strand, within ECOLIN_RS17685at 3562.616 kb on - strand, within ECOLIN_RS17685at 3562.859 kb on - strand, within ECOLIN_RS17685at 3562.937 kb on + strand, within ECOLIN_RS17685at 3562.938 kb on - strand, within ECOLIN_RS17685at 3563.041 kb on + strand, within ECOLIN_RS17685at 3563.041 kb on + strand, within ECOLIN_RS17685at 3563.338 kb on + strand, within ECOLIN_RS17685at 3563.338 kb on + strand, within ECOLIN_RS17685at 3563.366 kb on - strand, within ECOLIN_RS17685at 3563.525 kb on + strand, within ECOLIN_RS17685at 3563.561 kb on + strand, within ECOLIN_RS17685at 3563.562 kb on - strand, within ECOLIN_RS17685at 3563.564 kb on - strand, within ECOLIN_RS17685at 3563.674 kb on + strand, within ECOLIN_RS17685at 3563.675 kb on - strand, within ECOLIN_RS17685at 3563.682 kb on - strandat 3563.705 kb on + strandat 3563.770 kb on - strandat 3563.826 kb on - strandat 3563.863 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,561,497 + ECOLIN_RS17675 0.76 -0.3
3,561,780 + ECOLIN_RS17680 0.11 +0.2
3,561,780 + ECOLIN_RS17680 0.11 +0.8
3,562,132 + ECOLIN_RS17680 0.69 -0.2
3,562,132 + ECOLIN_RS17680 0.69 -0.0
3,562,208 + ECOLIN_RS17680 0.82 -0.1
3,562,209 - ECOLIN_RS17680 0.82 -0.5
3,562,211 - ECOLIN_RS17680 0.83 +0.7
3,562,211 - ECOLIN_RS17680 0.83 -0.1
3,562,279 + -0.0
3,562,280 - -0.3
3,562,315 - -2.4
3,562,358 - +0.0
3,562,429 - +0.1
3,562,429 - -0.2
3,562,429 - +0.3
3,562,459 + -0.3
3,562,459 + +0.1
3,562,459 + -0.9
3,562,459 + +0.0
3,562,549 - ECOLIN_RS17685 0.13 +0.8
3,562,549 - ECOLIN_RS17685 0.13 +0.3
3,562,615 + ECOLIN_RS17685 0.17 -4.3
3,562,615 + ECOLIN_RS17685 0.17 -1.3
3,562,616 - ECOLIN_RS17685 0.17 -0.2
3,562,859 - ECOLIN_RS17685 0.34 -1.2
3,562,937 + ECOLIN_RS17685 0.39 -0.2
3,562,938 - ECOLIN_RS17685 0.39 -0.0
3,563,041 + ECOLIN_RS17685 0.46 +0.0
3,563,041 + ECOLIN_RS17685 0.46 +0.1
3,563,338 + ECOLIN_RS17685 0.67 -0.2
3,563,338 + ECOLIN_RS17685 0.67 +0.4
3,563,366 - ECOLIN_RS17685 0.68 +1.0
3,563,525 + ECOLIN_RS17685 0.79 -0.6
3,563,561 + ECOLIN_RS17685 0.82 +0.3
3,563,562 - ECOLIN_RS17685 0.82 +0.3
3,563,564 - ECOLIN_RS17685 0.82 -1.9
3,563,674 + ECOLIN_RS17685 0.89 +0.3
3,563,675 - ECOLIN_RS17685 0.90 -0.3
3,563,682 - -0.1
3,563,705 + -0.4
3,563,770 - +0.2
3,563,826 - -0.8
3,563,863 - -0.1

Or see this region's nucleotide sequence