Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS14875

Experiment: D-Glucose

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS14860 and ECOLIN_RS14865 are separated by 83 nucleotidesECOLIN_RS14865 and ECOLIN_RS14870 are separated by 182 nucleotidesECOLIN_RS14870 and ECOLIN_RS14875 are separated by 9 nucleotidesECOLIN_RS14875 and ECOLIN_RS14880 are separated by 46 nucleotides ECOLIN_RS14860: ECOLIN_RS14860 - potassium binding protein Kbp, at 3,002,680 to 3,003,129 _RS14860 ECOLIN_RS14865: ECOLIN_RS14865 - YqaE/Pmp3 family membrane protein, at 3,003,213 to 3,003,371 _RS14865 ECOLIN_RS14870: ECOLIN_RS14870 - metalloregulator ArsR/SmtB family transcription factor, at 3,003,554 to 3,003,853 _RS14870 ECOLIN_RS14875: ECOLIN_RS14875 - thiosulfate sulfurtransferase YgaP, at 3,003,863 to 3,004,387 _RS14875 ECOLIN_RS14880: ECOLIN_RS14880 - DNA-binding protein StpA, at 3,004,434 to 3,004,838 _RS14880 Position (kb) 3003 3004 3005Strain fitness (log2 ratio) -2 -1 0 1at 3003.129 kb on + strandat 3003.133 kb on + strandat 3003.259 kb on + strand, within ECOLIN_RS14865at 3003.371 kb on + strandat 3003.472 kb on - strandat 3003.498 kb on + strandat 3003.499 kb on - strandat 3003.500 kb on + strandat 3003.553 kb on + strandat 3004.436 kb on - strandat 3004.461 kb on - strandat 3004.997 kb on - strandat 3005.158 kb on + strandat 3005.159 kb on - strandat 3005.199 kb on + strandat 3005.228 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose
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3,003,129 + +0.6
3,003,133 + +0.0
3,003,259 + ECOLIN_RS14865 0.29 +0.8
3,003,371 + +1.3
3,003,472 - -0.7
3,003,498 + -0.6
3,003,499 - +0.2
3,003,500 + +0.2
3,003,553 + +0.5
3,004,436 - +0.2
3,004,461 - +0.1
3,004,997 - +0.6
3,005,158 + -0.3
3,005,159 - -1.0
3,005,199 + -2.5
3,005,228 - -2.4

Or see this region's nucleotide sequence