Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS03400 and LRK55_RS03405 are separated by 76 nucleotides LRK55_RS03405 and LRK55_RS03410 are separated by 122 nucleotides LRK55_RS03410 and LRK55_RS03415 are separated by 137 nucleotides LRK55_RS03415 and LRK55_RS03420 are separated by 278 nucleotides
LRK55_RS03400: LRK55_RS03400 - elongation factor Tu, at 725,371 to 726,561
_RS03400
LRK55_RS03405: LRK55_RS03405 - tRNA-Thr, at 726,638 to 726,713
_RS03405
LRK55_RS03410: LRK55_RS03410 - tRNA-Gly, at 726,836 to 726,909
_RS03410
LRK55_RS03415: LRK55_RS03415 - tRNA-Tyr, at 727,047 to 727,132
_RS03415
LRK55_RS03420: LRK55_RS03420 - acyl-CoA thioesterase, at 727,411 to 727,899
_RS03420
Position (kb)
726
727 Strain fitness (log2 ratio)
-1
0
1
2 at 726.723 kb on - strand at 726.977 kb on - strand at 727.754 kb on + strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.755 kb on - strand, within LRK55_RS03420 at 727.784 kb on + strand, within LRK55_RS03420 at 727.890 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 726,723 - +0.9 726,977 - -0.8 727,754 + LRK55_RS03420 0.70 +0.7 727,755 - LRK55_RS03420 0.70 +0.7 727,755 - LRK55_RS03420 0.70 +0.2 727,755 - LRK55_RS03420 0.70 -0.5 727,755 - LRK55_RS03420 0.70 +2.2 727,755 - LRK55_RS03420 0.70 +1.7 727,784 + LRK55_RS03420 0.76 -0.5 727,890 + +0.8
Or see this region's nucleotide sequence