Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS02385 and LRK55_RS02390 are separated by 105 nucleotides LRK55_RS02390 and LRK55_RS02395 are separated by 168 nucleotides LRK55_RS02395 and LRK55_RS02400 are separated by 69 nucleotides
LRK55_RS02385: LRK55_RS02385 - MFS transporter, at 514,274 to 515,506
_RS02385
LRK55_RS02390: LRK55_RS02390 - class I SAM-dependent methyltransferase, at 515,612 to 516,268
_RS02390
LRK55_RS02395: LRK55_RS02395 - DEAD/DEAH box helicase, at 516,437 to 517,765
_RS02395
LRK55_RS02400: LRK55_RS02400 - S-formylglutathione hydrolase, at 517,835 to 518,683
_RS02400
Position (kb) 516
517
518 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 515.558 kb on + strand at 515.558 kb on + strand at 515.558 kb on + strand at 515.558 kb on + strand at 515.558 kb on + strand at 515.559 kb on - strand at 515.559 kb on - strand at 515.578 kb on + strand at 515.578 kb on + strand at 515.578 kb on + strand at 515.579 kb on - strand at 515.579 kb on - strand at 515.579 kb on - strand at 515.648 kb on + strand at 515.648 kb on + strand at 515.648 kb on + strand at 515.649 kb on - strand at 515.649 kb on - strand at 515.649 kb on - strand at 515.649 kb on - strand at 515.672 kb on + strand at 515.672 kb on + strand at 515.672 kb on + strand at 515.672 kb on + strand at 515.673 kb on - strand at 515.673 kb on - strand at 515.705 kb on + strand, within LRK55_RS02390 at 515.705 kb on + strand, within LRK55_RS02390 at 515.706 kb on - strand, within LRK55_RS02390 at 515.786 kb on + strand, within LRK55_RS02390 at 515.786 kb on + strand, within LRK55_RS02390 at 515.786 kb on + strand, within LRK55_RS02390 at 515.787 kb on - strand, within LRK55_RS02390 at 515.787 kb on - strand, within LRK55_RS02390 at 515.787 kb on - strand, within LRK55_RS02390 at 515.787 kb on - strand, within LRK55_RS02390 at 515.787 kb on - strand, within LRK55_RS02390 at 515.817 kb on - strand, within LRK55_RS02390 at 515.817 kb on - strand, within LRK55_RS02390 at 515.817 kb on - strand, within LRK55_RS02390 at 515.961 kb on + strand, within LRK55_RS02390 at 515.961 kb on + strand, within LRK55_RS02390 at 515.961 kb on + strand, within LRK55_RS02390 at 515.961 kb on + strand, within LRK55_RS02390 at 515.961 kb on + strand, within LRK55_RS02390 at 515.961 kb on + strand, within LRK55_RS02390 at 515.961 kb on + strand, within LRK55_RS02390 at 515.962 kb on - strand, within LRK55_RS02390 at 515.962 kb on - strand, within LRK55_RS02390 at 515.962 kb on - strand, within LRK55_RS02390 at 515.962 kb on - strand, within LRK55_RS02390 at 515.962 kb on - strand, within LRK55_RS02390 at 515.969 kb on + strand, within LRK55_RS02390 at 515.969 kb on + strand, within LRK55_RS02390 at 515.969 kb on + strand, within LRK55_RS02390 at 515.969 kb on + strand, within LRK55_RS02390 at 515.969 kb on + strand, within LRK55_RS02390 at 515.969 kb on + strand, within LRK55_RS02390 at 515.969 kb on + strand, within LRK55_RS02390 at 515.970 kb on - strand, within LRK55_RS02390 at 515.970 kb on - strand, within LRK55_RS02390 at 515.970 kb on - strand, within LRK55_RS02390 at 516.239 kb on + strand at 516.239 kb on + strand at 516.239 kb on + strand at 516.239 kb on + strand at 516.239 kb on + strand at 516.239 kb on + strand at 516.239 kb on + strand at 516.239 kb on + strand at 516.240 kb on - strand at 516.240 kb on - strand at 516.240 kb on - strand at 516.240 kb on - strand at 516.240 kb on - strand at 516.240 kb on - strand at 516.240 kb on - strand at 516.332 kb on + strand at 516.332 kb on + strand at 516.333 kb on - strand at 516.333 kb on - strand at 516.333 kb on - strand at 516.334 kb on + strand at 516.334 kb on + strand at 516.334 kb on + strand at 516.334 kb on + strand at 516.334 kb on + strand at 516.335 kb on - strand at 516.335 kb on - strand at 516.335 kb on - strand at 516.335 kb on - strand at 516.390 kb on + strand at 516.390 kb on + strand at 516.391 kb on - strand at 516.391 kb on - strand at 516.405 kb on + strand at 516.405 kb on + strand at 516.405 kb on + strand at 516.405 kb on + strand at 516.405 kb on + strand at 516.405 kb on + strand at 516.406 kb on - strand at 516.406 kb on - strand at 516.406 kb on - strand at 516.406 kb on - strand at 516.406 kb on - strand at 516.497 kb on + strand at 516.497 kb on + strand at 516.497 kb on + strand at 516.722 kb on + strand, within LRK55_RS02395 at 516.722 kb on + strand, within LRK55_RS02395 at 516.723 kb on - strand, within LRK55_RS02395 at 517.415 kb on + strand, within LRK55_RS02395 at 517.415 kb on + strand, within LRK55_RS02395 at 517.415 kb on + strand, within LRK55_RS02395 at 517.415 kb on + strand, within LRK55_RS02395 at 517.415 kb on + strand, within LRK55_RS02395 at 517.416 kb on - strand, within LRK55_RS02395 at 517.416 kb on - strand, within LRK55_RS02395 at 517.416 kb on - strand, within LRK55_RS02395 at 517.416 kb on - strand, within LRK55_RS02395 at 517.416 kb on - strand, within LRK55_RS02395 at 517.556 kb on + strand, within LRK55_RS02395 at 517.557 kb on - strand, within LRK55_RS02395 at 517.685 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.902 kb on + strand at 517.903 kb on - strand at 517.903 kb on - strand at 517.914 kb on + strand at 518.110 kb on - strand, within LRK55_RS02400 at 518.110 kb on - strand, within LRK55_RS02400 at 518.313 kb on + strand, within LRK55_RS02400 at 518.313 kb on + strand, within LRK55_RS02400 at 518.314 kb on - strand, within LRK55_RS02400 at 518.314 kb on - strand, within LRK55_RS02400 at 518.314 kb on - strand, within LRK55_RS02400 at 518.314 kb on - strand, within LRK55_RS02400 at 518.314 kb on - strand, within LRK55_RS02400 at 518.365 kb on - strand, within LRK55_RS02400 at 518.535 kb on + strand, within LRK55_RS02400 at 518.535 kb on + strand, within LRK55_RS02400 at 518.535 kb on + strand, within LRK55_RS02400 at 518.535 kb on + strand, within LRK55_RS02400 at 518.535 kb on + strand, within LRK55_RS02400 at 518.536 kb on - strand, within LRK55_RS02400 at 518.536 kb on - strand, within LRK55_RS02400 at 518.536 kb on - strand, within LRK55_RS02400 at 518.536 kb on - strand, within LRK55_RS02400 at 518.536 kb on - strand, within LRK55_RS02400 at 518.577 kb on + strand, within LRK55_RS02400 at 518.577 kb on + strand, within LRK55_RS02400 at 518.577 kb on + strand, within LRK55_RS02400 at 518.577 kb on + strand, within LRK55_RS02400 at 518.578 kb on - strand, within LRK55_RS02400 at 518.625 kb on + strand at 518.626 kb on - strand at 518.626 kb on - strand at 518.626 kb on - strand at 518.723 kb on - strand at 518.723 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 515,558 + -1.9 515,558 + +1.0 515,558 + -0.5 515,558 + +1.4 515,558 + -3.0 515,559 - +0.5 515,559 - -0.7 515,578 + +1.8 515,578 + -1.4 515,578 + +1.0 515,579 - +0.2 515,579 - +0.0 515,579 - -0.1 515,648 + -0.3 515,648 + -0.7 515,648 + +0.1 515,649 - -1.0 515,649 - -0.1 515,649 - +0.3 515,649 - -0.2 515,672 + -2.0 515,672 + +0.3 515,672 + +1.2 515,672 + +3.9 515,673 - -1.7 515,673 - +1.0 515,705 + LRK55_RS02390 0.14 +0.7 515,705 + LRK55_RS02390 0.14 -1.8 515,706 - LRK55_RS02390 0.14 -2.2 515,786 + LRK55_RS02390 0.26 -0.1 515,786 + LRK55_RS02390 0.26 -0.6 515,786 + LRK55_RS02390 0.26 +1.0 515,787 - LRK55_RS02390 0.27 +1.3 515,787 - LRK55_RS02390 0.27 +0.4 515,787 - LRK55_RS02390 0.27 +0.9 515,787 - LRK55_RS02390 0.27 -0.2 515,787 - LRK55_RS02390 0.27 +2.1 515,817 - LRK55_RS02390 0.31 -2.4 515,817 - LRK55_RS02390 0.31 +1.0 515,817 - LRK55_RS02390 0.31 +0.2 515,961 + LRK55_RS02390 0.53 +2.3 515,961 + LRK55_RS02390 0.53 -0.6 515,961 + LRK55_RS02390 0.53 +0.4 515,961 + LRK55_RS02390 0.53 -0.1 515,961 + LRK55_RS02390 0.53 -0.2 515,961 + LRK55_RS02390 0.53 -2.3 515,961 + LRK55_RS02390 0.53 -1.3 515,962 - LRK55_RS02390 0.53 +1.3 515,962 - LRK55_RS02390 0.53 +2.1 515,962 - LRK55_RS02390 0.53 -1.4 515,962 - LRK55_RS02390 0.53 -0.4 515,962 - LRK55_RS02390 0.53 +0.0 515,969 + LRK55_RS02390 0.54 +0.2 515,969 + LRK55_RS02390 0.54 -2.3 515,969 + LRK55_RS02390 0.54 -1.0 515,969 + LRK55_RS02390 0.54 -0.1 515,969 + LRK55_RS02390 0.54 -0.5 515,969 + LRK55_RS02390 0.54 -2.4 515,969 + LRK55_RS02390 0.54 -1.5 515,970 - LRK55_RS02390 0.54 -1.2 515,970 - LRK55_RS02390 0.54 +2.2 515,970 - LRK55_RS02390 0.54 +0.1 516,239 + +1.6 516,239 + +0.2 516,239 + -1.0 516,239 + -2.0 516,239 + +2.0 516,239 + -1.3 516,239 + +0.7 516,239 + -0.1 516,240 - +1.8 516,240 - +1.3 516,240 - -2.5 516,240 - +0.2 516,240 - -1.4 516,240 - -0.2 516,240 - +1.1 516,332 + -2.1 516,332 + +0.1 516,333 - -0.5 516,333 - +2.1 516,333 - -0.9 516,334 + -0.6 516,334 + +0.8 516,334 + +0.8 516,334 + -1.8 516,334 + -2.2 516,335 - -0.8 516,335 - +0.5 516,335 - +1.1 516,335 - -0.7 516,390 + +1.4 516,390 + +0.6 516,391 - +1.4 516,391 - +0.0 516,405 + -1.6 516,405 + +1.6 516,405 + +1.0 516,405 + -1.4 516,405 + +0.9 516,405 + +0.3 516,406 - +1.4 516,406 - +0.5 516,406 - +1.9 516,406 - +1.3 516,406 - -1.9 516,497 + -0.5 516,497 + +0.9 516,497 + +1.3 516,722 + LRK55_RS02395 0.21 +2.4 516,722 + LRK55_RS02395 0.21 +1.1 516,723 - LRK55_RS02395 0.22 -1.8 517,415 + LRK55_RS02395 0.74 -0.2 517,415 + LRK55_RS02395 0.74 -2.5 517,415 + LRK55_RS02395 0.74 +0.6 517,415 + LRK55_RS02395 0.74 -1.3 517,415 + LRK55_RS02395 0.74 -0.8 517,416 - LRK55_RS02395 0.74 -1.1 517,416 - LRK55_RS02395 0.74 +1.0 517,416 - LRK55_RS02395 0.74 +1.6 517,416 - LRK55_RS02395 0.74 -0.9 517,416 - LRK55_RS02395 0.74 +0.8 517,556 + LRK55_RS02395 0.84 +0.2 517,557 - LRK55_RS02395 0.84 +1.6 517,685 + -1.9 517,902 + +0.0 517,902 + -1.2 517,902 + -1.0 517,902 + +0.1 517,902 + +0.1 517,902 + +2.5 517,902 + +0.7 517,902 + -1.9 517,902 + -0.2 517,903 - +0.9 517,903 - -0.8 517,914 + +2.2 518,110 - LRK55_RS02400 0.32 -1.2 518,110 - LRK55_RS02400 0.32 -2.4 518,313 + LRK55_RS02400 0.56 +0.1 518,313 + LRK55_RS02400 0.56 +1.2 518,314 - LRK55_RS02400 0.56 +0.9 518,314 - LRK55_RS02400 0.56 +0.1 518,314 - LRK55_RS02400 0.56 +4.1 518,314 - LRK55_RS02400 0.56 +1.8 518,314 - LRK55_RS02400 0.56 -1.0 518,365 - LRK55_RS02400 0.62 -0.7 518,535 + LRK55_RS02400 0.82 -0.9 518,535 + LRK55_RS02400 0.82 +0.1 518,535 + LRK55_RS02400 0.82 +0.9 518,535 + LRK55_RS02400 0.82 +2.1 518,535 + LRK55_RS02400 0.82 -1.0 518,536 - LRK55_RS02400 0.83 +2.9 518,536 - LRK55_RS02400 0.83 -1.5 518,536 - LRK55_RS02400 0.83 +0.8 518,536 - LRK55_RS02400 0.83 -0.5 518,536 - LRK55_RS02400 0.83 +1.6 518,577 + LRK55_RS02400 0.87 +1.6 518,577 + LRK55_RS02400 0.87 +0.9 518,577 + LRK55_RS02400 0.87 -1.0 518,577 + LRK55_RS02400 0.87 +2.6 518,578 - LRK55_RS02400 0.88 -1.0 518,625 + -2.6 518,626 - +0.1 518,626 - -0.6 518,626 - +1.3 518,723 - +2.0 518,723 - -0.5
Or see this region's nucleotide sequence