Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS02005 and LRK55_RS02010 are separated by 222 nucleotides LRK55_RS02010 and LRK55_RS02015 are separated by 111 nucleotides LRK55_RS02015 and LRK55_RS02020 overlap by 4 nucleotides
LRK55_RS02005: LRK55_RS02005 - murein biosynthesis integral membrane protein MurJ, at 441,539 to 443,149
_RS02005
LRK55_RS02010: LRK55_RS02010 - 30S ribosomal protein S20, at 443,372 to 443,635
_RS02010
LRK55_RS02015: LRK55_RS02015 - hypothetical protein, at 443,747 to 444,049
_RS02015
LRK55_RS02020: LRK55_RS02020 - ferrous iron transporter B, at 444,046 to 445,881
_RS02020
Position (kb) 443
444 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 443.162 kb on + strand at 443.162 kb on + strand at 443.162 kb on + strand at 443.162 kb on + strand at 443.163 kb on - strand at 443.163 kb on - strand at 443.163 kb on - strand at 443.163 kb on - strand at 443.163 kb on - strand at 443.163 kb on - strand at 443.163 kb on - strand at 443.229 kb on + strand at 443.230 kb on - strand at 443.239 kb on + strand at 443.239 kb on + strand at 443.239 kb on + strand at 443.240 kb on - strand at 443.633 kb on + strand at 443.633 kb on + strand at 443.634 kb on - strand at 443.634 kb on - strand at 443.748 kb on + strand at 443.749 kb on - strand at 443.830 kb on + strand, within LRK55_RS02015 at 443.830 kb on + strand, within LRK55_RS02015 at 443.830 kb on + strand, within LRK55_RS02015 at 443.830 kb on + strand, within LRK55_RS02015 at 443.830 kb on + strand, within LRK55_RS02015 at 443.831 kb on - strand, within LRK55_RS02015 at 443.831 kb on - strand, within LRK55_RS02015 at 443.831 kb on - strand, within LRK55_RS02015 at 443.831 kb on - strand, within LRK55_RS02015 at 443.831 kb on - strand, within LRK55_RS02015 at 444.003 kb on - strand, within LRK55_RS02015 at 444.024 kb on + strand at 444.024 kb on + strand at 444.024 kb on + strand at 444.024 kb on + strand at 444.024 kb on + strand at 444.024 kb on + strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.025 kb on - strand at 444.071 kb on + strand at 444.071 kb on + strand at 444.071 kb on + strand at 444.071 kb on + strand at 444.071 kb on + strand at 444.071 kb on + strand at 444.072 kb on - strand at 444.092 kb on + strand at 444.092 kb on + strand at 444.092 kb on + strand at 444.092 kb on + strand at 444.093 kb on - strand at 444.093 kb on - strand at 444.093 kb on - strand at 444.093 kb on - strand at 444.110 kb on + strand at 444.110 kb on + strand at 444.110 kb on + strand at 444.110 kb on + strand at 444.110 kb on + strand at 444.110 kb on + strand at 444.110 kb on + strand at 444.111 kb on - strand at 444.111 kb on - strand at 444.111 kb on - strand at 444.111 kb on - strand at 444.111 kb on - strand at 444.111 kb on - strand at 444.111 kb on - strand at 444.111 kb on - strand at 444.191 kb on + strand at 444.191 kb on + strand at 444.192 kb on - strand at 444.192 kb on - strand at 444.192 kb on - strand at 444.232 kb on + strand, within LRK55_RS02020 at 444.233 kb on - strand, within LRK55_RS02020 at 444.233 kb on - strand, within LRK55_RS02020 at 444.233 kb on - strand, within LRK55_RS02020 at 444.233 kb on - strand, within LRK55_RS02020 at 444.284 kb on + strand, within LRK55_RS02020 at 444.284 kb on + strand, within LRK55_RS02020 at 444.285 kb on - strand, within LRK55_RS02020 at 444.285 kb on - strand, within LRK55_RS02020 at 444.285 kb on - strand, within LRK55_RS02020 at 444.285 kb on - strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.383 kb on + strand, within LRK55_RS02020 at 444.384 kb on - strand, within LRK55_RS02020 at 444.384 kb on - strand, within LRK55_RS02020 at 444.384 kb on - strand, within LRK55_RS02020 at 444.384 kb on - strand, within LRK55_RS02020 at 444.384 kb on - strand, within LRK55_RS02020 at 444.384 kb on - strand, within LRK55_RS02020 at 444.384 kb on - strand, within LRK55_RS02020 at 444.404 kb on + strand, within LRK55_RS02020 at 444.404 kb on + strand, within LRK55_RS02020 at 444.405 kb on - strand, within LRK55_RS02020 at 444.405 kb on - strand, within LRK55_RS02020 at 444.405 kb on - strand, within LRK55_RS02020 at 444.419 kb on + strand, within LRK55_RS02020 at 444.419 kb on + strand, within LRK55_RS02020 at 444.419 kb on + strand, within LRK55_RS02020 at 444.542 kb on + strand, within LRK55_RS02020 at 444.543 kb on - strand, within LRK55_RS02020 at 444.543 kb on - strand, within LRK55_RS02020 at 444.543 kb on - strand, within LRK55_RS02020 at 444.543 kb on - strand, within LRK55_RS02020 at 444.582 kb on - strand, within LRK55_RS02020
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 443,162 + +0.6 443,162 + -2.0 443,162 + +0.2 443,162 + -3.3 443,163 - +0.9 443,163 - -1.0 443,163 - -1.9 443,163 - +2.3 443,163 - +0.6 443,163 - -2.1 443,163 - +0.9 443,229 + -1.7 443,230 - -1.9 443,239 + -1.4 443,239 + -1.8 443,239 + +0.9 443,240 - +1.5 443,633 + +1.0 443,633 + +0.9 443,634 - -0.6 443,634 - -1.7 443,748 + -0.8 443,749 - -0.0 443,830 + LRK55_RS02015 0.27 -3.5 443,830 + LRK55_RS02015 0.27 -4.0 443,830 + LRK55_RS02015 0.27 -2.1 443,830 + LRK55_RS02015 0.27 -1.0 443,830 + LRK55_RS02015 0.27 -2.6 443,831 - LRK55_RS02015 0.28 -3.6 443,831 - LRK55_RS02015 0.28 -4.2 443,831 - LRK55_RS02015 0.28 -1.5 443,831 - LRK55_RS02015 0.28 -3.4 443,831 - LRK55_RS02015 0.28 -4.8 444,003 - LRK55_RS02015 0.84 -2.9 444,024 + -2.7 444,024 + -1.9 444,024 + -1.7 444,024 + -1.5 444,024 + -3.5 444,024 + -2.2 444,025 - -3.0 444,025 - -2.5 444,025 - -1.7 444,025 - -1.9 444,025 - -4.0 444,025 - -3.7 444,025 - -2.9 444,025 - -3.3 444,025 - -1.0 444,071 + -2.7 444,071 + -0.8 444,071 + -0.5 444,071 + +0.5 444,071 + -0.8 444,071 + -3.0 444,072 - -1.8 444,092 + -2.3 444,092 + -1.0 444,092 + -3.7 444,092 + -2.9 444,093 - -3.6 444,093 - -2.2 444,093 - -4.3 444,093 - -1.4 444,110 + -3.9 444,110 + -2.9 444,110 + -0.5 444,110 + -1.7 444,110 + -3.0 444,110 + -3.7 444,110 + -2.2 444,111 - -1.8 444,111 - -2.9 444,111 - -1.5 444,111 - -2.9 444,111 - -4.2 444,111 - -0.2 444,111 - -4.1 444,111 - -1.5 444,191 + -2.1 444,191 + -1.4 444,192 - -1.4 444,192 - -3.5 444,192 - -0.5 444,232 + LRK55_RS02020 0.10 -0.2 444,233 - LRK55_RS02020 0.10 -3.7 444,233 - LRK55_RS02020 0.10 -1.0 444,233 - LRK55_RS02020 0.10 -1.9 444,233 - LRK55_RS02020 0.10 -1.4 444,284 + LRK55_RS02020 0.13 -3.4 444,284 + LRK55_RS02020 0.13 -2.4 444,285 - LRK55_RS02020 0.13 -1.5 444,285 - LRK55_RS02020 0.13 -0.5 444,285 - LRK55_RS02020 0.13 -3.4 444,285 - LRK55_RS02020 0.13 -0.5 444,383 + LRK55_RS02020 0.18 -3.8 444,383 + LRK55_RS02020 0.18 -1.7 444,383 + LRK55_RS02020 0.18 -1.4 444,383 + LRK55_RS02020 0.18 -3.5 444,383 + LRK55_RS02020 0.18 -1.6 444,383 + LRK55_RS02020 0.18 -2.5 444,383 + LRK55_RS02020 0.18 -3.6 444,383 + LRK55_RS02020 0.18 -3.0 444,383 + LRK55_RS02020 0.18 -2.9 444,383 + LRK55_RS02020 0.18 -2.0 444,383 + LRK55_RS02020 0.18 -4.1 444,383 + LRK55_RS02020 0.18 -2.9 444,383 + LRK55_RS02020 0.18 -3.1 444,383 + LRK55_RS02020 0.18 -3.2 444,384 - LRK55_RS02020 0.18 -0.2 444,384 - LRK55_RS02020 0.18 -2.1 444,384 - LRK55_RS02020 0.18 -3.7 444,384 - LRK55_RS02020 0.18 -4.6 444,384 - LRK55_RS02020 0.18 -2.5 444,384 - LRK55_RS02020 0.18 -2.8 444,384 - LRK55_RS02020 0.18 -1.7 444,404 + LRK55_RS02020 0.19 -2.2 444,404 + LRK55_RS02020 0.19 -1.4 444,405 - LRK55_RS02020 0.20 -3.4 444,405 - LRK55_RS02020 0.20 +0.4 444,405 - LRK55_RS02020 0.20 -1.2 444,419 + LRK55_RS02020 0.20 -1.4 444,419 + LRK55_RS02020 0.20 -1.6 444,419 + LRK55_RS02020 0.20 -3.9 444,542 + LRK55_RS02020 0.27 -2.0 444,543 - LRK55_RS02020 0.27 -1.9 444,543 - LRK55_RS02020 0.27 -0.3 444,543 - LRK55_RS02020 0.27 -1.1 444,543 - LRK55_RS02020 0.27 -3.1 444,582 - LRK55_RS02020 0.29 -3.9
Or see this region's nucleotide sequence