Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS01020 and LRK55_RS01025 are separated by 109 nucleotides LRK55_RS01025 and LRK55_RS01030 overlap by 1 nucleotides LRK55_RS01030 and LRK55_RS01040 are separated by 224 nucleotides LRK55_RS01040 and LRK55_RS01045 are separated by 94 nucleotides
LRK55_RS01020: LRK55_RS01020 - recombination mediator RecR, at 223,601 to 224,200
_RS01020
LRK55_RS01025: LRK55_RS01025 - YbaB/EbfC family nucleoid-associated protein, at 224,310 to 224,633
_RS01025
LRK55_RS01030: LRK55_RS01030 - DNA polymerase III subunit gamma/tau, at 224,633 to 226,318
_RS01030
LRK55_RS01040: LRK55_RS01040 - tRNA-Ser, at 226,543 to 226,635
_RS01040
LRK55_RS01045: LRK55_RS01045 - MBL fold metallo-hydrolase, at 226,730 to 227,488
_RS01045
Position (kb) 224
225
226
227 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 223.656 kb on + strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.656 kb on + strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.657 kb on - strand at 223.737 kb on + strand, within LRK55_RS01020 at 223.737 kb on + strand, within LRK55_RS01020 at 223.737 kb on + strand, within LRK55_RS01020 at 223.738 kb on - strand, within LRK55_RS01020 at 223.738 kb on - strand, within LRK55_RS01020 at 223.890 kb on + strand, within LRK55_RS01020 at 223.890 kb on + strand, within LRK55_RS01020 at 223.890 kb on + strand, within LRK55_RS01020 at 223.890 kb on + strand, within LRK55_RS01020 at 223.890 kb on + strand, within LRK55_RS01020 at 223.890 kb on + strand, within LRK55_RS01020 at 223.890 kb on + strand, within LRK55_RS01020 at 223.891 kb on - strand, within LRK55_RS01020 at 223.891 kb on - strand, within LRK55_RS01020 at 223.937 kb on - strand, within LRK55_RS01020 at 224.220 kb on - strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.291 kb on + strand at 224.292 kb on - strand at 224.292 kb on - strand at 224.292 kb on - strand at 226.374 kb on + strand at 226.374 kb on + strand at 226.374 kb on + strand at 226.375 kb on - strand at 226.375 kb on - strand at 226.375 kb on - strand at 226.375 kb on - strand at 226.848 kb on + strand, within LRK55_RS01045 at 226.848 kb on + strand, within LRK55_RS01045 at 226.848 kb on + strand, within LRK55_RS01045 at 226.848 kb on + strand, within LRK55_RS01045 at 226.849 kb on - strand, within LRK55_RS01045 at 226.849 kb on - strand, within LRK55_RS01045 at 226.849 kb on - strand, within LRK55_RS01045 at 226.881 kb on + strand, within LRK55_RS01045 at 227.040 kb on + strand, within LRK55_RS01045 at 227.054 kb on + strand, within LRK55_RS01045 at 227.055 kb on - strand, within LRK55_RS01045 at 227.176 kb on - strand, within LRK55_RS01045 at 227.308 kb on + strand, within LRK55_RS01045 at 227.308 kb on + strand, within LRK55_RS01045 at 227.309 kb on - strand, within LRK55_RS01045
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 223,656 + -1.0 223,656 + +0.8 223,656 + +1.5 223,656 + -2.2 223,656 + +0.8 223,656 + -0.9 223,656 + -0.7 223,657 - +1.6 223,657 - +0.6 223,657 - -1.0 223,657 - -0.1 223,657 - -3.6 223,657 - -0.7 223,657 - +0.7 223,657 - -1.7 223,657 - +1.3 223,737 + LRK55_RS01020 0.23 +2.0 223,737 + LRK55_RS01020 0.23 -2.1 223,737 + LRK55_RS01020 0.23 -0.6 223,738 - LRK55_RS01020 0.23 +0.8 223,738 - LRK55_RS01020 0.23 -0.2 223,890 + LRK55_RS01020 0.48 +0.8 223,890 + LRK55_RS01020 0.48 -0.7 223,890 + LRK55_RS01020 0.48 +0.1 223,890 + LRK55_RS01020 0.48 +1.3 223,890 + LRK55_RS01020 0.48 -3.3 223,890 + LRK55_RS01020 0.48 -0.4 223,890 + LRK55_RS01020 0.48 -2.3 223,891 - LRK55_RS01020 0.48 +0.2 223,891 - LRK55_RS01020 0.48 +0.6 223,937 - LRK55_RS01020 0.56 -1.7 224,220 - +0.3 224,291 + +0.5 224,291 + -0.4 224,291 + -2.8 224,291 + +0.1 224,291 + +1.3 224,291 + -0.4 224,291 + -0.4 224,291 + +0.8 224,291 + -3.7 224,291 + -1.7 224,291 + -1.8 224,292 - -0.4 224,292 - -1.6 224,292 - -1.5 226,374 + -1.0 226,374 + +1.8 226,374 + -2.4 226,375 - +0.3 226,375 - +1.5 226,375 - -1.0 226,375 - -1.0 226,848 + LRK55_RS01045 0.16 -0.6 226,848 + LRK55_RS01045 0.16 -0.2 226,848 + LRK55_RS01045 0.16 -1.0 226,848 + LRK55_RS01045 0.16 +0.2 226,849 - LRK55_RS01045 0.16 -0.8 226,849 - LRK55_RS01045 0.16 -1.0 226,849 - LRK55_RS01045 0.16 -0.8 226,881 + LRK55_RS01045 0.20 -0.2 227,040 + LRK55_RS01045 0.41 -0.6 227,054 + LRK55_RS01045 0.43 -1.6 227,055 - LRK55_RS01045 0.43 -0.2 227,176 - LRK55_RS01045 0.59 +0.2 227,308 + LRK55_RS01045 0.76 -0.8 227,308 + LRK55_RS01045 0.76 -0.6 227,309 - LRK55_RS01045 0.76 -1.2
Or see this region's nucleotide sequence