Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS18665 and LRK55_RS18670 are separated by 1 nucleotides LRK55_RS18670 and LRK55_RS18675 overlap by 8 nucleotides LRK55_RS18675 and LRK55_RS18680 overlap by 4 nucleotides
LRK55_RS18665: LRK55_RS18665 - efflux RND transporter periplasmic adaptor subunit, at 68,517 to 69,578
_RS18665
LRK55_RS18670: LRK55_RS18670 - CusA/CzcA family heavy metal efflux RND transporter, at 69,580 to 72,663
_RS18670
LRK55_RS18675: LRK55_RS18675 - DUF3240 family protein, at 72,656 to 72,973
_RS18675
LRK55_RS18680: LRK55_RS18680 - TolC family protein, at 72,970 to 74,214
_RS18680
Position (kb) 69
70
71
72
73 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 68.594 kb on + strand at 68.595 kb on - strand at 68.595 kb on - strand at 68.641 kb on - strand, within LRK55_RS18665 at 69.308 kb on - strand, within LRK55_RS18665 at 69.408 kb on + strand, within LRK55_RS18665 at 69.409 kb on - strand, within LRK55_RS18665 at 69.409 kb on - strand, within LRK55_RS18665 at 69.409 kb on - strand, within LRK55_RS18665 at 69.490 kb on + strand at 69.491 kb on - strand at 69.745 kb on - strand at 69.745 kb on - strand at 69.745 kb on - strand at 69.841 kb on + strand at 69.842 kb on - strand at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.890 kb on - strand, within LRK55_RS18670 at 69.890 kb on - strand, within LRK55_RS18670 at 70.133 kb on - strand, within LRK55_RS18670 at 70.134 kb on - strand, within LRK55_RS18670 at 70.134 kb on - strand, within LRK55_RS18670 at 70.543 kb on + strand, within LRK55_RS18670 at 70.544 kb on - strand, within LRK55_RS18670 at 70.544 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.925 kb on + strand, within LRK55_RS18670 at 71.435 kb on + strand, within LRK55_RS18670 at 71.501 kb on + strand, within LRK55_RS18670 at 71.527 kb on + strand, within LRK55_RS18670 at 71.527 kb on + strand, within LRK55_RS18670 at 71.528 kb on - strand, within LRK55_RS18670 at 71.528 kb on - strand, within LRK55_RS18670 at 71.689 kb on + strand, within LRK55_RS18670 at 71.690 kb on - strand, within LRK55_RS18670 at 71.716 kb on + strand, within LRK55_RS18670 at 71.717 kb on - strand, within LRK55_RS18670 at 71.717 kb on - strand, within LRK55_RS18670 at 71.769 kb on - strand, within LRK55_RS18670 at 72.231 kb on - strand, within LRK55_RS18670 at 72.310 kb on + strand, within LRK55_RS18670 at 72.310 kb on + strand, within LRK55_RS18670 at 72.311 kb on - strand, within LRK55_RS18670 at 72.388 kb on + strand at 72.388 kb on + strand at 72.389 kb on - strand at 72.389 kb on - strand at 72.619 kb on + strand at 72.620 kb on - strand at 72.620 kb on - strand at 72.650 kb on + strand at 72.650 kb on + strand at 72.651 kb on - strand at 72.651 kb on - strand at 72.651 kb on - strand at 72.651 kb on - strand at 72.661 kb on + strand at 72.661 kb on + strand at 72.661 kb on + strand at 72.662 kb on - strand at 72.848 kb on + strand, within LRK55_RS18675 at 72.849 kb on - strand, within LRK55_RS18675 at 73.541 kb on + strand, within LRK55_RS18680 at 73.623 kb on + strand, within LRK55_RS18680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 68,594 + -1.2 68,595 - -3.1 68,595 - -1.6 68,641 - LRK55_RS18665 0.12 -1.2 69,308 - LRK55_RS18665 0.74 -0.8 69,408 + LRK55_RS18665 0.84 -3.5 69,409 - LRK55_RS18665 0.84 +2.2 69,409 - LRK55_RS18665 0.84 +0.9 69,409 - LRK55_RS18665 0.84 -1.3 69,490 + +0.9 69,491 - +0.5 69,745 - -1.6 69,745 - -1.8 69,745 - -1.2 69,841 + +0.1 69,842 - +1.9 69,889 + LRK55_RS18670 0.10 +0.2 69,889 + LRK55_RS18670 0.10 -0.1 69,889 + LRK55_RS18670 0.10 +0.7 69,889 + LRK55_RS18670 0.10 +0.4 69,889 + LRK55_RS18670 0.10 -1.2 69,889 + LRK55_RS18670 0.10 -3.2 69,890 - LRK55_RS18670 0.10 +0.3 69,890 - LRK55_RS18670 0.10 +1.8 70,133 - LRK55_RS18670 0.18 +1.9 70,134 - LRK55_RS18670 0.18 -0.2 70,134 - LRK55_RS18670 0.18 +0.8 70,543 + LRK55_RS18670 0.31 +1.0 70,544 - LRK55_RS18670 0.31 -1.2 70,544 - LRK55_RS18670 0.31 +1.4 70,650 - LRK55_RS18670 0.35 +0.5 70,650 - LRK55_RS18670 0.35 +0.3 70,650 - LRK55_RS18670 0.35 -0.3 70,650 - LRK55_RS18670 0.35 -1.2 70,925 + LRK55_RS18670 0.44 +2.5 71,435 + LRK55_RS18670 0.60 +0.2 71,501 + LRK55_RS18670 0.62 +1.6 71,527 + LRK55_RS18670 0.63 -2.7 71,527 + LRK55_RS18670 0.63 -0.5 71,528 - LRK55_RS18670 0.63 +1.5 71,528 - LRK55_RS18670 0.63 +0.8 71,689 + LRK55_RS18670 0.68 +0.9 71,690 - LRK55_RS18670 0.68 +1.8 71,716 + LRK55_RS18670 0.69 +1.1 71,717 - LRK55_RS18670 0.69 +0.6 71,717 - LRK55_RS18670 0.69 +1.2 71,769 - LRK55_RS18670 0.71 +1.7 72,231 - LRK55_RS18670 0.86 +1.5 72,310 + LRK55_RS18670 0.89 -1.3 72,310 + LRK55_RS18670 0.89 -4.9 72,311 - LRK55_RS18670 0.89 +0.1 72,388 + -2.8 72,388 + -1.8 72,389 - -1.2 72,389 - -2.1 72,619 + -1.6 72,620 - -1.6 72,620 - +1.0 72,650 + +0.4 72,650 + +0.7 72,651 - -2.7 72,651 - -4.0 72,651 - -0.3 72,651 - -1.9 72,661 + -2.8 72,661 + +2.0 72,661 + +0.6 72,662 - -0.8 72,848 + LRK55_RS18675 0.60 -1.1 72,849 - LRK55_RS18675 0.61 -1.7 73,541 + LRK55_RS18680 0.46 +0.3 73,623 + LRK55_RS18680 0.52 -0.8
Or see this region's nucleotide sequence