Experiment: R2A_PIPES pH 4.25
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS14225 and LRK55_RS14230 are separated by 11 nucleotides LRK55_RS14230 and LRK55_RS14235 are separated by 83 nucleotides LRK55_RS14235 and LRK55_RS14240 are separated by 49 nucleotides LRK55_RS14240 and LRK55_RS14245 overlap by 4 nucleotides
LRK55_RS14225: LRK55_RS14225 - copper chaperone PCu(A)C, at 3,145,926 to 3,146,444
_RS14225
LRK55_RS14230: LRK55_RS14230 - SCO family protein, at 3,146,456 to 3,147,073
_RS14230
LRK55_RS14235: LRK55_RS14235 - DUF423 domain-containing protein, at 3,147,157 to 3,147,534
_RS14235
LRK55_RS14240: LRK55_RS14240 - hypothetical protein, at 3,147,584 to 3,147,997
_RS14240
LRK55_RS14245: LRK55_RS14245 - CPBP family glutamic-type intramembrane protease, at 3,147,994 to 3,148,764
_RS14245
Position (kb)
3147
3148 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3146.429 kb on + strand at 3146.429 kb on + strand at 3146.429 kb on + strand at 3146.429 kb on + strand at 3146.429 kb on + strand at 3146.430 kb on - strand at 3146.430 kb on - strand at 3146.430 kb on - strand at 3146.565 kb on + strand, within LRK55_RS14230 at 3146.566 kb on - strand, within LRK55_RS14230 at 3146.566 kb on - strand, within LRK55_RS14230 at 3146.566 kb on - strand, within LRK55_RS14230 at 3146.566 kb on - strand, within LRK55_RS14230 at 3146.593 kb on - strand, within LRK55_RS14230 at 3146.658 kb on - strand, within LRK55_RS14230 at 3146.658 kb on - strand, within LRK55_RS14230 at 3146.712 kb on + strand, within LRK55_RS14230 at 3146.712 kb on + strand, within LRK55_RS14230 at 3146.713 kb on - strand, within LRK55_RS14230 at 3146.857 kb on - strand, within LRK55_RS14230 at 3146.865 kb on + strand, within LRK55_RS14230 at 3146.865 kb on + strand, within LRK55_RS14230 at 3146.865 kb on + strand, within LRK55_RS14230 at 3146.866 kb on - strand, within LRK55_RS14230 at 3146.866 kb on - strand, within LRK55_RS14230 at 3147.106 kb on + strand at 3147.106 kb on + strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.107 kb on - strand at 3147.174 kb on + strand at 3147.288 kb on + strand, within LRK55_RS14235 at 3147.288 kb on + strand, within LRK55_RS14235 at 3147.301 kb on + strand, within LRK55_RS14235 at 3147.301 kb on + strand, within LRK55_RS14235 at 3147.301 kb on + strand, within LRK55_RS14235 at 3147.301 kb on + strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.302 kb on - strand, within LRK55_RS14235 at 3147.418 kb on + strand, within LRK55_RS14235 at 3147.532 kb on + strand at 3147.532 kb on + strand at 3147.532 kb on + strand at 3147.532 kb on + strand at 3147.533 kb on - strand at 3147.533 kb on - strand at 3147.824 kb on + strand, within LRK55_RS14240 at 3147.995 kb on + strand at 3148.076 kb on + strand, within LRK55_RS14245 at 3148.076 kb on + strand, within LRK55_RS14245 at 3148.491 kb on - strand, within LRK55_RS14245 at 3148.526 kb on + strand, within LRK55_RS14245 at 3148.526 kb on + strand, within LRK55_RS14245 at 3148.527 kb on - strand, within LRK55_RS14245 at 3148.527 kb on - strand, within LRK55_RS14245
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES pH 4.25 remove 3,146,429 + +0.9 3,146,429 + +2.0 3,146,429 + +3.2 3,146,429 + +4.1 3,146,429 + +2.4 3,146,430 - +4.0 3,146,430 - +3.3 3,146,430 - +2.2 3,146,565 + LRK55_RS14230 0.18 +0.0 3,146,566 - LRK55_RS14230 0.18 +1.2 3,146,566 - LRK55_RS14230 0.18 -0.4 3,146,566 - LRK55_RS14230 0.18 +0.8 3,146,566 - LRK55_RS14230 0.18 +0.0 3,146,593 - LRK55_RS14230 0.22 +1.8 3,146,658 - LRK55_RS14230 0.33 -1.4 3,146,658 - LRK55_RS14230 0.33 +2.1 3,146,712 + LRK55_RS14230 0.41 +2.5 3,146,712 + LRK55_RS14230 0.41 +1.6 3,146,713 - LRK55_RS14230 0.42 +0.3 3,146,857 - LRK55_RS14230 0.65 +0.6 3,146,865 + LRK55_RS14230 0.66 +0.2 3,146,865 + LRK55_RS14230 0.66 +2.5 3,146,865 + LRK55_RS14230 0.66 +1.0 3,146,866 - LRK55_RS14230 0.66 +1.9 3,146,866 - LRK55_RS14230 0.66 +1.6 3,147,106 + -0.4 3,147,106 + +0.5 3,147,107 - +1.2 3,147,107 - -2.0 3,147,107 - +1.0 3,147,107 - +0.4 3,147,107 - +0.9 3,147,107 - +2.0 3,147,107 - +2.2 3,147,107 - +1.2 3,147,107 - +1.4 3,147,174 + -2.6 3,147,288 + LRK55_RS14235 0.35 +0.1 3,147,288 + LRK55_RS14235 0.35 +0.6 3,147,301 + LRK55_RS14235 0.38 +1.0 3,147,301 + LRK55_RS14235 0.38 +1.0 3,147,301 + LRK55_RS14235 0.38 +1.4 3,147,301 + LRK55_RS14235 0.38 +0.3 3,147,302 - LRK55_RS14235 0.38 +0.4 3,147,302 - LRK55_RS14235 0.38 +0.9 3,147,302 - LRK55_RS14235 0.38 +1.3 3,147,302 - LRK55_RS14235 0.38 -1.7 3,147,302 - LRK55_RS14235 0.38 +0.0 3,147,302 - LRK55_RS14235 0.38 -2.9 3,147,302 - LRK55_RS14235 0.38 +2.1 3,147,302 - LRK55_RS14235 0.38 +0.0 3,147,418 + LRK55_RS14235 0.69 -0.6 3,147,532 + -0.7 3,147,532 + +3.5 3,147,532 + +0.6 3,147,532 + -1.7 3,147,533 - -0.4 3,147,533 - +1.1 3,147,824 + LRK55_RS14240 0.58 +3.1 3,147,995 + +0.9 3,148,076 + LRK55_RS14245 0.11 +1.7 3,148,076 + LRK55_RS14245 0.11 +1.7 3,148,491 - LRK55_RS14245 0.64 +1.3 3,148,526 + LRK55_RS14245 0.69 +0.7 3,148,526 + LRK55_RS14245 0.69 -1.6 3,148,527 - LRK55_RS14245 0.69 +0.3 3,148,527 - LRK55_RS14245 0.69 -0.7
Or see this region's nucleotide sequence