Experiment: R2A with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS18410 and LRK55_RS18415 are separated by 147 nucleotides LRK55_RS18415 and LRK55_RS18420 are separated by 243 nucleotides LRK55_RS18420 and LRK55_RS18425 are separated by 76 nucleotides
LRK55_RS18410: LRK55_RS18410 - PRTRC system ThiF family protein, at 15,059 to 15,868
_RS18410
LRK55_RS18415: LRK55_RS18415 - single-stranded DNA-binding protein, at 16,016 to 16,600
_RS18415
LRK55_RS18420: LRK55_RS18420 - DEAD/DEAH box helicase, at 16,844 to 18,841
_RS18420
LRK55_RS18425: LRK55_RS18425 - tetratricopeptide repeat protein, at 18,918 to 19,829
_RS18425
Position (kb)
16
17
18
19 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 15.895 kb on + strand at 15.896 kb on - strand at 15.896 kb on - strand at 15.950 kb on + strand at 15.951 kb on - strand at 15.951 kb on - strand at 15.951 kb on - strand at 15.951 kb on - strand at 16.016 kb on - strand at 16.016 kb on - strand at 16.250 kb on + strand, within LRK55_RS18415 at 16.250 kb on + strand, within LRK55_RS18415 at 16.250 kb on + strand, within LRK55_RS18415 at 16.250 kb on + strand, within LRK55_RS18415 at 16.251 kb on - strand, within LRK55_RS18415 at 16.251 kb on - strand, within LRK55_RS18415 at 16.251 kb on - strand, within LRK55_RS18415 at 16.251 kb on - strand, within LRK55_RS18415 at 16.251 kb on - strand, within LRK55_RS18415 at 16.280 kb on + strand, within LRK55_RS18415 at 16.281 kb on - strand, within LRK55_RS18415 at 16.308 kb on - strand, within LRK55_RS18415 at 16.446 kb on - strand, within LRK55_RS18415 at 16.545 kb on - strand at 16.725 kb on + strand at 16.725 kb on + strand at 16.725 kb on + strand at 16.835 kb on + strand at 16.835 kb on + strand at 16.835 kb on + strand at 16.835 kb on + strand at 16.835 kb on + strand at 16.835 kb on + strand at 16.835 kb on + strand at 16.836 kb on - strand at 16.836 kb on - strand at 16.946 kb on + strand at 16.947 kb on - strand at 17.345 kb on + strand, within LRK55_RS18420 at 17.345 kb on + strand, within LRK55_RS18420 at 17.345 kb on + strand, within LRK55_RS18420 at 17.346 kb on - strand, within LRK55_RS18420 at 17.379 kb on + strand, within LRK55_RS18420 at 17.379 kb on + strand, within LRK55_RS18420 at 17.379 kb on + strand, within LRK55_RS18420 at 17.379 kb on + strand, within LRK55_RS18420 at 17.379 kb on + strand, within LRK55_RS18420 at 17.380 kb on - strand, within LRK55_RS18420 at 17.430 kb on + strand, within LRK55_RS18420 at 17.430 kb on + strand, within LRK55_RS18420 at 17.430 kb on + strand, within LRK55_RS18420 at 17.430 kb on + strand, within LRK55_RS18420 at 17.431 kb on - strand, within LRK55_RS18420 at 17.431 kb on - strand, within LRK55_RS18420 at 17.431 kb on - strand, within LRK55_RS18420 at 17.431 kb on - strand, within LRK55_RS18420 at 17.431 kb on - strand, within LRK55_RS18420 at 17.431 kb on - strand, within LRK55_RS18420 at 17.431 kb on - strand, within LRK55_RS18420 at 17.465 kb on + strand, within LRK55_RS18420 at 17.465 kb on + strand, within LRK55_RS18420 at 17.465 kb on + strand, within LRK55_RS18420 at 17.466 kb on - strand, within LRK55_RS18420 at 17.682 kb on + strand, within LRK55_RS18420 at 17.783 kb on + strand, within LRK55_RS18420 at 17.784 kb on - strand, within LRK55_RS18420 at 17.993 kb on + strand, within LRK55_RS18420 at 17.993 kb on + strand, within LRK55_RS18420 at 17.993 kb on + strand, within LRK55_RS18420 at 17.993 kb on + strand, within LRK55_RS18420 at 17.993 kb on + strand, within LRK55_RS18420 at 17.993 kb on + strand, within LRK55_RS18420 at 17.994 kb on - strand, within LRK55_RS18420 at 17.994 kb on - strand, within LRK55_RS18420 at 18.161 kb on + strand, within LRK55_RS18420 at 18.161 kb on + strand, within LRK55_RS18420 at 18.162 kb on - strand, within LRK55_RS18420 at 18.283 kb on + strand, within LRK55_RS18420 at 18.284 kb on - strand, within LRK55_RS18420 at 18.326 kb on + strand, within LRK55_RS18420 at 18.326 kb on + strand, within LRK55_RS18420 at 18.327 kb on - strand, within LRK55_RS18420 at 18.327 kb on - strand, within LRK55_RS18420 at 18.327 kb on - strand, within LRK55_RS18420 at 18.347 kb on - strand, within LRK55_RS18420 at 18.467 kb on + strand, within LRK55_RS18420 at 18.467 kb on + strand, within LRK55_RS18420 at 18.497 kb on + strand, within LRK55_RS18420 at 18.626 kb on - strand, within LRK55_RS18420 at 18.626 kb on - strand, within LRK55_RS18420 at 18.882 kb on - strand at 18.949 kb on + strand at 18.950 kb on - strand at 18.950 kb on - strand at 18.950 kb on - strand at 18.950 kb on - strand at 18.971 kb on - strand at 19.154 kb on + strand, within LRK55_RS18425 at 19.154 kb on + strand, within LRK55_RS18425 at 19.155 kb on - strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.596 kb on + strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.597 kb on - strand, within LRK55_RS18425 at 19.645 kb on + strand, within LRK55_RS18425 at 19.645 kb on + strand, within LRK55_RS18425 at 19.645 kb on + strand, within LRK55_RS18425 at 19.646 kb on - strand, within LRK55_RS18425 at 19.710 kb on + strand, within LRK55_RS18425 at 19.710 kb on + strand, within LRK55_RS18425 at 19.749 kb on + strand at 19.758 kb on + strand at 19.758 kb on + strand at 19.759 kb on - strand at 19.827 kb on + strand at 19.828 kb on - strand at 19.828 kb on - strand at 19.828 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Metal_ion_mix_sitestressplate 2 x remove 15,895 + -0.5 15,896 - -0.4 15,896 - -0.9 15,950 + +0.8 15,951 - +0.1 15,951 - -1.5 15,951 - -0.0 15,951 - +0.2 16,016 - -0.3 16,016 - -0.7 16,250 + LRK55_RS18415 0.40 -0.9 16,250 + LRK55_RS18415 0.40 +0.5 16,250 + LRK55_RS18415 0.40 +0.1 16,250 + LRK55_RS18415 0.40 -1.1 16,251 - LRK55_RS18415 0.40 +0.7 16,251 - LRK55_RS18415 0.40 -0.2 16,251 - LRK55_RS18415 0.40 -0.8 16,251 - LRK55_RS18415 0.40 -1.3 16,251 - LRK55_RS18415 0.40 +1.0 16,280 + LRK55_RS18415 0.45 -1.6 16,281 - LRK55_RS18415 0.45 +0.1 16,308 - LRK55_RS18415 0.50 -2.5 16,446 - LRK55_RS18415 0.74 +0.3 16,545 - -1.5 16,725 + +0.8 16,725 + -0.4 16,725 + -0.0 16,835 + -0.0 16,835 + +1.5 16,835 + -0.2 16,835 + -0.2 16,835 + -1.3 16,835 + -0.2 16,835 + +0.5 16,836 - -1.4 16,836 - +0.2 16,946 + +0.0 16,947 - +1.0 17,345 + LRK55_RS18420 0.25 +0.3 17,345 + LRK55_RS18420 0.25 -0.5 17,345 + LRK55_RS18420 0.25 -0.2 17,346 - LRK55_RS18420 0.25 -0.8 17,379 + LRK55_RS18420 0.27 +0.9 17,379 + LRK55_RS18420 0.27 -0.6 17,379 + LRK55_RS18420 0.27 -0.1 17,379 + LRK55_RS18420 0.27 -0.3 17,379 + LRK55_RS18420 0.27 -0.6 17,380 - LRK55_RS18420 0.27 -0.6 17,430 + LRK55_RS18420 0.29 -0.1 17,430 + LRK55_RS18420 0.29 -0.6 17,430 + LRK55_RS18420 0.29 -0.6 17,430 + LRK55_RS18420 0.29 -0.4 17,431 - LRK55_RS18420 0.29 +1.8 17,431 - LRK55_RS18420 0.29 -0.0 17,431 - LRK55_RS18420 0.29 +2.1 17,431 - LRK55_RS18420 0.29 +0.6 17,431 - LRK55_RS18420 0.29 +0.1 17,431 - LRK55_RS18420 0.29 -0.1 17,431 - LRK55_RS18420 0.29 -0.7 17,465 + LRK55_RS18420 0.31 +1.6 17,465 + LRK55_RS18420 0.31 +0.5 17,465 + LRK55_RS18420 0.31 -0.8 17,466 - LRK55_RS18420 0.31 +0.1 17,682 + LRK55_RS18420 0.42 -2.5 17,783 + LRK55_RS18420 0.47 -0.2 17,784 - LRK55_RS18420 0.47 -0.5 17,993 + LRK55_RS18420 0.58 -0.0 17,993 + LRK55_RS18420 0.58 +1.4 17,993 + LRK55_RS18420 0.58 +0.4 17,993 + LRK55_RS18420 0.58 +0.1 17,993 + LRK55_RS18420 0.58 +0.1 17,993 + LRK55_RS18420 0.58 -0.5 17,994 - LRK55_RS18420 0.58 +0.8 17,994 - LRK55_RS18420 0.58 +0.2 18,161 + LRK55_RS18420 0.66 +0.2 18,161 + LRK55_RS18420 0.66 -1.2 18,162 - LRK55_RS18420 0.66 -0.8 18,283 + LRK55_RS18420 0.72 -0.7 18,284 - LRK55_RS18420 0.72 +0.2 18,326 + LRK55_RS18420 0.74 +1.7 18,326 + LRK55_RS18420 0.74 +1.0 18,327 - LRK55_RS18420 0.74 -0.6 18,327 - LRK55_RS18420 0.74 -0.4 18,327 - LRK55_RS18420 0.74 +0.6 18,347 - LRK55_RS18420 0.75 -0.2 18,467 + LRK55_RS18420 0.81 -0.4 18,467 + LRK55_RS18420 0.81 -0.2 18,497 + LRK55_RS18420 0.83 -1.5 18,626 - LRK55_RS18420 0.89 -0.1 18,626 - LRK55_RS18420 0.89 -0.3 18,882 - -0.5 18,949 + -0.1 18,950 - +0.1 18,950 - +1.2 18,950 - -0.4 18,950 - +1.0 18,971 - -0.2 19,154 + LRK55_RS18425 0.26 -0.2 19,154 + LRK55_RS18425 0.26 +0.0 19,155 - LRK55_RS18425 0.26 -2.9 19,596 + LRK55_RS18425 0.74 -1.4 19,596 + LRK55_RS18425 0.74 -0.1 19,596 + LRK55_RS18425 0.74 +0.4 19,596 + LRK55_RS18425 0.74 +1.1 19,596 + LRK55_RS18425 0.74 -0.1 19,596 + LRK55_RS18425 0.74 +0.5 19,596 + LRK55_RS18425 0.74 +0.3 19,596 + LRK55_RS18425 0.74 -0.5 19,596 + LRK55_RS18425 0.74 +0.7 19,597 - LRK55_RS18425 0.74 -0.0 19,597 - LRK55_RS18425 0.74 -0.5 19,597 - LRK55_RS18425 0.74 -0.3 19,597 - LRK55_RS18425 0.74 +0.4 19,597 - LRK55_RS18425 0.74 +0.1 19,597 - LRK55_RS18425 0.74 -0.9 19,597 - LRK55_RS18425 0.74 +0.3 19,597 - LRK55_RS18425 0.74 +0.9 19,645 + LRK55_RS18425 0.80 +0.1 19,645 + LRK55_RS18425 0.80 +0.6 19,645 + LRK55_RS18425 0.80 +1.0 19,646 - LRK55_RS18425 0.80 -0.9 19,710 + LRK55_RS18425 0.87 +1.1 19,710 + LRK55_RS18425 0.87 +0.1 19,749 + +1.0 19,758 + -0.5 19,758 + +0.7 19,759 - +0.4 19,827 + +1.5 19,828 - +0.1 19,828 - +0.6 19,828 - -0.2
Or see this region's nucleotide sequence