Experiment: R2A with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS01740 and LRK55_RS01745 are separated by 81 nucleotides LRK55_RS01745 and LRK55_RS01750 are separated by 61 nucleotides LRK55_RS01750 and LRK55_RS01755 are separated by 14 nucleotides
LRK55_RS01740: LRK55_RS01740 - TolC family protein, at 368,236 to 369,507
_RS01740
LRK55_RS01745: LRK55_RS01745 - hypothetical protein, at 369,589 to 370,017
_RS01745
LRK55_RS01750: LRK55_RS01750 - DUF411 domain-containing protein, at 370,079 to 370,531
_RS01750
LRK55_RS01755: LRK55_RS01755 - c-type cytochrome, at 370,546 to 371,619
_RS01755
Position (kb) 370
371 Strain fitness (log2 ratio) -2
-1
0
1
2 at 369.232 kb on + strand, within LRK55_RS01740 at 369.232 kb on + strand, within LRK55_RS01740 at 369.233 kb on - strand, within LRK55_RS01740 at 369.515 kb on + strand at 369.515 kb on + strand at 369.515 kb on + strand at 369.515 kb on + strand at 369.516 kb on - strand at 369.516 kb on - strand at 369.516 kb on - strand at 369.516 kb on - strand at 369.516 kb on - strand at 369.516 kb on - strand at 369.516 kb on - strand at 369.516 kb on - strand at 369.741 kb on + strand, within LRK55_RS01745 at 369.920 kb on + strand, within LRK55_RS01745 at 369.920 kb on + strand, within LRK55_RS01745 at 369.920 kb on + strand, within LRK55_RS01745 at 369.920 kb on + strand, within LRK55_RS01745 at 369.921 kb on - strand, within LRK55_RS01745 at 369.921 kb on - strand, within LRK55_RS01745 at 369.921 kb on - strand, within LRK55_RS01745 at 369.921 kb on - strand, within LRK55_RS01745 at 370.031 kb on + strand at 370.031 kb on + strand at 370.147 kb on - strand, within LRK55_RS01750 at 370.159 kb on + strand, within LRK55_RS01750 at 370.159 kb on + strand, within LRK55_RS01750 at 370.159 kb on + strand, within LRK55_RS01750 at 370.279 kb on - strand, within LRK55_RS01750 at 370.279 kb on - strand, within LRK55_RS01750 at 370.279 kb on - strand, within LRK55_RS01750 at 370.279 kb on - strand, within LRK55_RS01750 at 370.380 kb on + strand, within LRK55_RS01750 at 370.381 kb on - strand, within LRK55_RS01750 at 370.381 kb on - strand, within LRK55_RS01750 at 370.398 kb on + strand, within LRK55_RS01750 at 370.398 kb on + strand, within LRK55_RS01750 at 370.398 kb on + strand, within LRK55_RS01750 at 370.398 kb on + strand, within LRK55_RS01750 at 370.399 kb on - strand, within LRK55_RS01750 at 370.441 kb on - strand, within LRK55_RS01750 at 370.790 kb on + strand, within LRK55_RS01755 at 370.790 kb on + strand, within LRK55_RS01755 at 370.791 kb on - strand, within LRK55_RS01755 at 370.791 kb on - strand, within LRK55_RS01755 at 370.791 kb on - strand, within LRK55_RS01755 at 371.144 kb on + strand, within LRK55_RS01755 at 371.145 kb on - strand, within LRK55_RS01755 at 371.321 kb on + strand, within LRK55_RS01755 at 371.390 kb on + strand, within LRK55_RS01755 at 371.390 kb on + strand, within LRK55_RS01755 at 371.499 kb on - strand, within LRK55_RS01755
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Metal_ion_mix_sitestressplate 2 x remove 369,232 + LRK55_RS01740 0.78 -1.2 369,232 + LRK55_RS01740 0.78 +0.1 369,233 - LRK55_RS01740 0.78 +0.2 369,515 + -0.3 369,515 + +1.0 369,515 + -0.0 369,515 + +0.3 369,516 - +0.7 369,516 - -0.4 369,516 - +0.7 369,516 - -1.8 369,516 - +0.1 369,516 - +1.9 369,516 - +0.4 369,516 - +0.9 369,741 + LRK55_RS01745 0.35 -0.8 369,920 + LRK55_RS01745 0.77 +0.3 369,920 + LRK55_RS01745 0.77 -0.2 369,920 + LRK55_RS01745 0.77 +1.2 369,920 + LRK55_RS01745 0.77 +0.8 369,921 - LRK55_RS01745 0.77 +0.7 369,921 - LRK55_RS01745 0.77 +0.2 369,921 - LRK55_RS01745 0.77 -1.2 369,921 - LRK55_RS01745 0.77 -1.9 370,031 + -0.2 370,031 + -0.1 370,147 - LRK55_RS01750 0.15 -0.1 370,159 + LRK55_RS01750 0.18 -0.2 370,159 + LRK55_RS01750 0.18 +1.0 370,159 + LRK55_RS01750 0.18 -1.6 370,279 - LRK55_RS01750 0.44 +0.8 370,279 - LRK55_RS01750 0.44 -0.3 370,279 - LRK55_RS01750 0.44 -0.9 370,279 - LRK55_RS01750 0.44 +0.6 370,380 + LRK55_RS01750 0.66 +0.2 370,381 - LRK55_RS01750 0.67 -1.1 370,381 - LRK55_RS01750 0.67 +0.1 370,398 + LRK55_RS01750 0.70 -1.3 370,398 + LRK55_RS01750 0.70 +0.5 370,398 + LRK55_RS01750 0.70 -2.2 370,398 + LRK55_RS01750 0.70 -1.0 370,399 - LRK55_RS01750 0.71 -1.6 370,441 - LRK55_RS01750 0.80 +1.6 370,790 + LRK55_RS01755 0.23 +0.0 370,790 + LRK55_RS01755 0.23 -0.1 370,791 - LRK55_RS01755 0.23 +0.8 370,791 - LRK55_RS01755 0.23 +0.5 370,791 - LRK55_RS01755 0.23 -0.1 371,144 + LRK55_RS01755 0.56 -0.7 371,145 - LRK55_RS01755 0.56 +0.7 371,321 + LRK55_RS01755 0.72 +0.4 371,390 + LRK55_RS01755 0.79 -2.7 371,390 + LRK55_RS01755 0.79 +1.1 371,499 - LRK55_RS01755 0.89 -0.8
Or see this region's nucleotide sequence