Experiment: R2A with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS18665 and LRK55_RS18670 are separated by 1 nucleotides LRK55_RS18670 and LRK55_RS18675 overlap by 8 nucleotides LRK55_RS18675 and LRK55_RS18680 overlap by 4 nucleotides
LRK55_RS18665: LRK55_RS18665 - efflux RND transporter periplasmic adaptor subunit, at 68,517 to 69,578
_RS18665
LRK55_RS18670: LRK55_RS18670 - CusA/CzcA family heavy metal efflux RND transporter, at 69,580 to 72,663
_RS18670
LRK55_RS18675: LRK55_RS18675 - DUF3240 family protein, at 72,656 to 72,973
_RS18675
LRK55_RS18680: LRK55_RS18680 - TolC family protein, at 72,970 to 74,214
_RS18680
Position (kb)
69
70
71
72
73 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 68.594 kb on + strand at 68.595 kb on - strand at 68.595 kb on - strand at 68.641 kb on - strand, within LRK55_RS18665 at 69.308 kb on - strand, within LRK55_RS18665 at 69.408 kb on + strand, within LRK55_RS18665 at 69.409 kb on - strand, within LRK55_RS18665 at 69.409 kb on - strand, within LRK55_RS18665 at 69.409 kb on - strand, within LRK55_RS18665 at 69.490 kb on + strand at 69.491 kb on - strand at 69.745 kb on - strand at 69.745 kb on - strand at 69.745 kb on - strand at 69.841 kb on + strand at 69.842 kb on - strand at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.889 kb on + strand, within LRK55_RS18670 at 69.890 kb on - strand, within LRK55_RS18670 at 69.890 kb on - strand, within LRK55_RS18670 at 70.133 kb on - strand, within LRK55_RS18670 at 70.134 kb on - strand, within LRK55_RS18670 at 70.134 kb on - strand, within LRK55_RS18670 at 70.543 kb on + strand, within LRK55_RS18670 at 70.544 kb on - strand, within LRK55_RS18670 at 70.544 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.650 kb on - strand, within LRK55_RS18670 at 70.925 kb on + strand, within LRK55_RS18670 at 71.435 kb on + strand, within LRK55_RS18670 at 71.501 kb on + strand, within LRK55_RS18670 at 71.527 kb on + strand, within LRK55_RS18670 at 71.527 kb on + strand, within LRK55_RS18670 at 71.528 kb on - strand, within LRK55_RS18670 at 71.528 kb on - strand, within LRK55_RS18670 at 71.689 kb on + strand, within LRK55_RS18670 at 71.690 kb on - strand, within LRK55_RS18670 at 71.716 kb on + strand, within LRK55_RS18670 at 71.717 kb on - strand, within LRK55_RS18670 at 71.717 kb on - strand, within LRK55_RS18670 at 71.769 kb on - strand, within LRK55_RS18670 at 72.231 kb on - strand, within LRK55_RS18670 at 72.310 kb on + strand, within LRK55_RS18670 at 72.310 kb on + strand, within LRK55_RS18670 at 72.311 kb on - strand, within LRK55_RS18670 at 72.388 kb on + strand at 72.388 kb on + strand at 72.389 kb on - strand at 72.389 kb on - strand at 72.619 kb on + strand at 72.620 kb on - strand at 72.620 kb on - strand at 72.650 kb on + strand at 72.650 kb on + strand at 72.651 kb on - strand at 72.651 kb on - strand at 72.651 kb on - strand at 72.651 kb on - strand at 72.661 kb on + strand at 72.661 kb on + strand at 72.661 kb on + strand at 72.662 kb on - strand at 72.848 kb on + strand, within LRK55_RS18675 at 72.849 kb on - strand, within LRK55_RS18675 at 73.541 kb on + strand, within LRK55_RS18680 at 73.623 kb on + strand, within LRK55_RS18680
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Metal_ion_mix_sitestressplate 2 x remove 68,594 + -0.7 68,595 - -0.0 68,595 - -0.8 68,641 - LRK55_RS18665 0.12 +0.1 69,308 - LRK55_RS18665 0.74 -1.1 69,408 + LRK55_RS18665 0.84 -0.7 69,409 - LRK55_RS18665 0.84 +0.7 69,409 - LRK55_RS18665 0.84 +0.9 69,409 - LRK55_RS18665 0.84 -2.1 69,490 + +0.0 69,491 - -2.3 69,745 - +1.2 69,745 - -0.3 69,745 - -0.7 69,841 + -0.9 69,842 - -0.5 69,889 + LRK55_RS18670 0.10 -0.4 69,889 + LRK55_RS18670 0.10 -0.4 69,889 + LRK55_RS18670 0.10 -1.1 69,889 + LRK55_RS18670 0.10 -1.1 69,889 + LRK55_RS18670 0.10 -1.7 69,889 + LRK55_RS18670 0.10 -0.7 69,890 - LRK55_RS18670 0.10 -1.5 69,890 - LRK55_RS18670 0.10 +0.7 70,133 - LRK55_RS18670 0.18 +0.3 70,134 - LRK55_RS18670 0.18 -0.3 70,134 - LRK55_RS18670 0.18 -0.7 70,543 + LRK55_RS18670 0.31 -0.7 70,544 - LRK55_RS18670 0.31 +0.3 70,544 - LRK55_RS18670 0.31 +0.4 70,650 - LRK55_RS18670 0.35 -0.5 70,650 - LRK55_RS18670 0.35 -0.6 70,650 - LRK55_RS18670 0.35 -0.3 70,650 - LRK55_RS18670 0.35 -0.7 70,925 + LRK55_RS18670 0.44 -0.0 71,435 + LRK55_RS18670 0.60 -0.6 71,501 + LRK55_RS18670 0.62 +0.1 71,527 + LRK55_RS18670 0.63 -0.2 71,527 + LRK55_RS18670 0.63 +0.5 71,528 - LRK55_RS18670 0.63 -1.7 71,528 - LRK55_RS18670 0.63 -0.8 71,689 + LRK55_RS18670 0.68 -1.2 71,690 - LRK55_RS18670 0.68 -0.9 71,716 + LRK55_RS18670 0.69 +0.1 71,717 - LRK55_RS18670 0.69 -0.2 71,717 - LRK55_RS18670 0.69 -0.6 71,769 - LRK55_RS18670 0.71 -0.6 72,231 - LRK55_RS18670 0.86 +0.2 72,310 + LRK55_RS18670 0.89 -3.4 72,310 + LRK55_RS18670 0.89 -1.2 72,311 - LRK55_RS18670 0.89 -0.5 72,388 + -0.2 72,388 + -0.2 72,389 - -0.7 72,389 - +1.1 72,619 + +0.3 72,620 - -0.1 72,620 - -1.1 72,650 + -0.6 72,650 + -0.4 72,651 - -1.5 72,651 - -0.7 72,651 - -2.4 72,651 - +1.4 72,661 + -1.0 72,661 + +0.3 72,661 + -1.0 72,662 - -1.2 72,848 + LRK55_RS18675 0.60 -0.9 72,849 - LRK55_RS18675 0.61 -1.1 73,541 + LRK55_RS18680 0.46 -0.4 73,623 + LRK55_RS18680 0.52 -0.9
Or see this region's nucleotide sequence