Experiment: R2A with Metal_ion_mix_sitestressplate 2 x
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS14375 and LRK55_RS14380 are separated by 127 nucleotides LRK55_RS14380 and LRK55_RS14385 are separated by 4 nucleotides LRK55_RS14385 and LRK55_RS14390 are separated by 2 nucleotides LRK55_RS14390 and LRK55_RS14395 are separated by 184 nucleotides
LRK55_RS14375: LRK55_RS14375 - cytochrome c biogenesis protein DipZ, at 3,170,694 to 3,172,415
_RS14375
LRK55_RS14380: LRK55_RS14380 - sigma-70 family RNA polymerase sigma factor, at 3,172,543 to 3,173,208
_RS14380
LRK55_RS14385: LRK55_RS14385 - NrsF family protein, at 3,173,213 to 3,173,866
_RS14385
LRK55_RS14390: LRK55_RS14390 - YajQ family cyclic di-GMP-binding protein, at 3,173,869 to 3,174,351
_RS14390
LRK55_RS14395: LRK55_RS14395 - NAD(P)/FAD-dependent oxidoreductase, at 3,174,536 to 3,175,714
_RS14395
Position (kb)
3173
3174 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3172.381 kb on - strand at 3172.381 kb on - strand at 3172.381 kb on - strand at 3172.381 kb on - strand at 3172.381 kb on - strand at 3172.393 kb on + strand at 3172.394 kb on - strand at 3172.394 kb on - strand at 3172.444 kb on + strand at 3172.444 kb on + strand at 3172.470 kb on - strand at 3172.486 kb on + strand at 3172.486 kb on + strand at 3172.486 kb on + strand at 3172.486 kb on + strand at 3172.487 kb on - strand at 3172.487 kb on - strand at 3172.487 kb on - strand at 3172.487 kb on - strand at 3172.487 kb on - strand at 3172.487 kb on - strand at 3172.487 kb on - strand at 3172.512 kb on - strand at 3172.580 kb on + strand at 3172.580 kb on + strand at 3172.580 kb on + strand at 3172.581 kb on - strand at 3172.584 kb on + strand at 3172.584 kb on + strand at 3172.584 kb on + strand at 3172.585 kb on - strand at 3172.585 kb on - strand at 3172.585 kb on - strand at 3172.585 kb on - strand at 3172.589 kb on + strand at 3172.589 kb on + strand at 3172.589 kb on + strand at 3172.589 kb on + strand at 3172.590 kb on - strand at 3172.590 kb on - strand at 3172.590 kb on - strand at 3172.590 kb on - strand at 3172.590 kb on - strand at 3172.590 kb on - strand at 3172.590 kb on - strand at 3172.718 kb on - strand, within LRK55_RS14380 at 3172.718 kb on - strand, within LRK55_RS14380 at 3172.846 kb on + strand, within LRK55_RS14380 at 3172.846 kb on + strand, within LRK55_RS14380 at 3172.846 kb on + strand, within LRK55_RS14380 at 3172.846 kb on + strand, within LRK55_RS14380 at 3172.846 kb on + strand, within LRK55_RS14380 at 3172.846 kb on + strand, within LRK55_RS14380 at 3172.847 kb on - strand, within LRK55_RS14380 at 3172.847 kb on - strand, within LRK55_RS14380 at 3172.847 kb on - strand, within LRK55_RS14380 at 3172.847 kb on - strand, within LRK55_RS14380 at 3172.864 kb on + strand, within LRK55_RS14380 at 3172.864 kb on + strand, within LRK55_RS14380 at 3172.864 kb on + strand, within LRK55_RS14380 at 3172.865 kb on - strand, within LRK55_RS14380 at 3172.865 kb on - strand, within LRK55_RS14380 at 3173.302 kb on + strand, within LRK55_RS14385 at 3173.357 kb on + strand, within LRK55_RS14385 at 3173.358 kb on - strand, within LRK55_RS14385 at 3173.358 kb on - strand, within LRK55_RS14385 at 3173.392 kb on + strand, within LRK55_RS14385 at 3173.392 kb on + strand, within LRK55_RS14385 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.236 kb on + strand, within LRK55_RS14390 at 3174.237 kb on - strand, within LRK55_RS14390 at 3174.237 kb on - strand, within LRK55_RS14390 at 3174.237 kb on - strand, within LRK55_RS14390 at 3174.237 kb on - strand, within LRK55_RS14390 at 3174.237 kb on - strand, within LRK55_RS14390 at 3174.384 kb on + strand at 3174.384 kb on + strand at 3174.384 kb on + strand at 3174.384 kb on + strand at 3174.384 kb on + strand at 3174.385 kb on - strand at 3174.385 kb on - strand at 3174.385 kb on - strand at 3174.385 kb on - strand at 3174.385 kb on - strand at 3174.385 kb on - strand at 3174.385 kb on - strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.459 kb on + strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.460 kb on - strand at 3174.472 kb on + strand at 3174.472 kb on + strand at 3174.473 kb on - strand at 3174.473 kb on - strand at 3174.473 kb on - strand at 3174.473 kb on - strand at 3174.530 kb on + strand at 3174.530 kb on + strand at 3174.530 kb on + strand at 3174.530 kb on + strand at 3174.530 kb on + strand at 3174.530 kb on + strand at 3174.530 kb on + strand at 3174.530 kb on + strand at 3174.531 kb on - strand at 3174.531 kb on - strand at 3174.531 kb on - strand at 3174.531 kb on - strand at 3174.716 kb on + strand, within LRK55_RS14395 at 3174.716 kb on + strand, within LRK55_RS14395 at 3174.716 kb on + strand, within LRK55_RS14395 at 3174.716 kb on + strand, within LRK55_RS14395 at 3174.716 kb on + strand, within LRK55_RS14395 at 3174.717 kb on - strand, within LRK55_RS14395 at 3174.717 kb on - strand, within LRK55_RS14395 at 3174.717 kb on - strand, within LRK55_RS14395 at 3174.717 kb on - strand, within LRK55_RS14395 at 3174.717 kb on - strand, within LRK55_RS14395 at 3174.717 kb on - strand, within LRK55_RS14395 at 3174.717 kb on - strand, within LRK55_RS14395 at 3174.762 kb on - strand, within LRK55_RS14395 at 3174.809 kb on + strand, within LRK55_RS14395 at 3174.809 kb on + strand, within LRK55_RS14395
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Metal_ion_mix_sitestressplate 2 x remove 3,172,381 - -0.5 3,172,381 - +0.5 3,172,381 - +1.4 3,172,381 - +0.1 3,172,381 - +0.9 3,172,393 + -0.1 3,172,394 - -0.8 3,172,394 - +0.4 3,172,444 + +0.6 3,172,444 + -0.5 3,172,470 - -0.2 3,172,486 + +0.5 3,172,486 + -0.5 3,172,486 + -0.2 3,172,486 + +1.3 3,172,487 - -1.1 3,172,487 - +0.7 3,172,487 - -0.5 3,172,487 - +1.1 3,172,487 - -0.3 3,172,487 - +1.1 3,172,487 - +0.1 3,172,512 - +0.8 3,172,580 + -0.2 3,172,580 + -3.1 3,172,580 + -0.5 3,172,581 - +0.6 3,172,584 + -0.2 3,172,584 + -1.0 3,172,584 + +0.0 3,172,585 - -0.7 3,172,585 - +0.0 3,172,585 - +0.3 3,172,585 - +1.1 3,172,589 + +0.2 3,172,589 + +0.1 3,172,589 + +0.9 3,172,589 + +0.6 3,172,590 - -0.8 3,172,590 - -3.6 3,172,590 - +0.5 3,172,590 - -0.4 3,172,590 - +0.3 3,172,590 - +1.0 3,172,590 - -0.2 3,172,718 - LRK55_RS14380 0.26 -1.5 3,172,718 - LRK55_RS14380 0.26 +0.1 3,172,846 + LRK55_RS14380 0.45 -0.2 3,172,846 + LRK55_RS14380 0.45 +0.6 3,172,846 + LRK55_RS14380 0.45 +0.3 3,172,846 + LRK55_RS14380 0.45 -0.2 3,172,846 + LRK55_RS14380 0.45 +0.6 3,172,846 + LRK55_RS14380 0.45 -0.8 3,172,847 - LRK55_RS14380 0.46 -1.0 3,172,847 - LRK55_RS14380 0.46 -0.8 3,172,847 - LRK55_RS14380 0.46 -0.4 3,172,847 - LRK55_RS14380 0.46 -0.4 3,172,864 + LRK55_RS14380 0.48 -1.5 3,172,864 + LRK55_RS14380 0.48 -0.8 3,172,864 + LRK55_RS14380 0.48 +0.9 3,172,865 - LRK55_RS14380 0.48 +0.1 3,172,865 - LRK55_RS14380 0.48 +1.4 3,173,302 + LRK55_RS14385 0.14 -1.8 3,173,357 + LRK55_RS14385 0.22 +0.7 3,173,358 - LRK55_RS14385 0.22 -2.0 3,173,358 - LRK55_RS14385 0.22 -2.2 3,173,392 + LRK55_RS14385 0.27 -1.1 3,173,392 + LRK55_RS14385 0.27 -0.2 3,174,236 + LRK55_RS14390 0.76 -0.8 3,174,236 + LRK55_RS14390 0.76 +1.1 3,174,236 + LRK55_RS14390 0.76 -0.8 3,174,236 + LRK55_RS14390 0.76 +0.9 3,174,236 + LRK55_RS14390 0.76 -1.5 3,174,236 + LRK55_RS14390 0.76 -0.5 3,174,236 + LRK55_RS14390 0.76 -0.1 3,174,236 + LRK55_RS14390 0.76 +0.1 3,174,237 - LRK55_RS14390 0.76 +0.7 3,174,237 - LRK55_RS14390 0.76 +0.2 3,174,237 - LRK55_RS14390 0.76 -0.5 3,174,237 - LRK55_RS14390 0.76 -0.0 3,174,237 - LRK55_RS14390 0.76 +0.1 3,174,384 + +1.2 3,174,384 + +0.7 3,174,384 + +1.9 3,174,384 + +1.3 3,174,384 + +1.7 3,174,385 - +0.2 3,174,385 - +0.0 3,174,385 - +0.3 3,174,385 - -0.8 3,174,385 - -1.7 3,174,385 - +0.5 3,174,385 - -0.5 3,174,459 + -0.2 3,174,459 + -1.0 3,174,459 + -0.9 3,174,459 + -0.2 3,174,459 + +0.4 3,174,459 + +1.1 3,174,459 + +0.6 3,174,459 + -1.7 3,174,459 + -0.1 3,174,459 + -0.2 3,174,460 - -0.2 3,174,460 - -0.9 3,174,460 - +0.0 3,174,460 - -1.0 3,174,460 - +0.6 3,174,460 - +0.3 3,174,460 - -0.4 3,174,460 - +0.8 3,174,460 - -0.6 3,174,460 - -0.0 3,174,460 - -1.0 3,174,460 - +0.6 3,174,472 + -3.0 3,174,472 + +1.0 3,174,473 - +0.0 3,174,473 - -0.4 3,174,473 - -0.7 3,174,473 - -1.0 3,174,530 + -1.0 3,174,530 + +0.8 3,174,530 + +1.3 3,174,530 + -0.2 3,174,530 + -0.6 3,174,530 + -1.0 3,174,530 + +0.1 3,174,530 + +0.6 3,174,531 - +1.3 3,174,531 - -1.5 3,174,531 - -0.0 3,174,531 - +0.8 3,174,716 + LRK55_RS14395 0.15 +0.3 3,174,716 + LRK55_RS14395 0.15 +1.3 3,174,716 + LRK55_RS14395 0.15 -0.3 3,174,716 + LRK55_RS14395 0.15 +1.3 3,174,716 + LRK55_RS14395 0.15 -1.8 3,174,717 - LRK55_RS14395 0.15 -1.2 3,174,717 - LRK55_RS14395 0.15 +1.6 3,174,717 - LRK55_RS14395 0.15 -2.7 3,174,717 - LRK55_RS14395 0.15 +1.1 3,174,717 - LRK55_RS14395 0.15 -0.5 3,174,717 - LRK55_RS14395 0.15 -0.2 3,174,717 - LRK55_RS14395 0.15 -2.1 3,174,762 - LRK55_RS14395 0.19 -0.3 3,174,809 + LRK55_RS14395 0.23 +0.2 3,174,809 + LRK55_RS14395 0.23 +0.7
Or see this region's nucleotide sequence