Experiment: R2A with Zinc chloride 2560 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS19260 and LRK55_RS19265 are separated by 67 nucleotides LRK55_RS19265 and LRK55_RS19270 are separated by 15 nucleotides LRK55_RS19270 and LRK55_RS19275 are separated by 85 nucleotides
LRK55_RS19260: LRK55_RS19260 - type IV secretion system protein TraC, at 196,896 to 199,325
_RS19260
LRK55_RS19265: LRK55_RS19265 - TraA family conjugative transfer protein, at 199,393 to 199,773
_RS19265
LRK55_RS19270: LRK55_RS19270 - TraV family lipoprotein, at 199,789 to 200,547
_RS19270
LRK55_RS19275: LRK55_RS19275 - TraB/VirB10 family protein, at 200,633 to 201,913
_RS19275
Position (kb) 199
200
201 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 198.796 kb on + strand, within LRK55_RS19260 at 198.797 kb on - strand, within LRK55_RS19260 at 198.797 kb on - strand, within LRK55_RS19260 at 198.797 kb on - strand, within LRK55_RS19260 at 198.797 kb on - strand, within LRK55_RS19260 at 198.797 kb on - strand, within LRK55_RS19260 at 199.003 kb on + strand, within LRK55_RS19260 at 199.004 kb on - strand, within LRK55_RS19260 at 199.315 kb on + strand at 199.319 kb on + strand at 199.319 kb on + strand at 199.320 kb on - strand at 199.436 kb on + strand, within LRK55_RS19265 at 199.436 kb on + strand, within LRK55_RS19265 at 199.436 kb on + strand, within LRK55_RS19265 at 199.437 kb on - strand, within LRK55_RS19265 at 199.580 kb on + strand, within LRK55_RS19265 at 199.580 kb on + strand, within LRK55_RS19265 at 199.581 kb on - strand, within LRK55_RS19265 at 199.581 kb on - strand, within LRK55_RS19265 at 199.836 kb on + strand at 199.837 kb on - strand at 199.837 kb on - strand at 200.012 kb on + strand, within LRK55_RS19270 at 200.078 kb on - strand, within LRK55_RS19270 at 200.219 kb on + strand, within LRK55_RS19270 at 200.220 kb on - strand, within LRK55_RS19270 at 200.414 kb on + strand, within LRK55_RS19270 at 200.415 kb on - strand, within LRK55_RS19270 at 200.415 kb on - strand, within LRK55_RS19270 at 200.433 kb on + strand, within LRK55_RS19270 at 200.433 kb on + strand, within LRK55_RS19270 at 200.538 kb on + strand at 200.614 kb on + strand at 200.614 kb on + strand at 200.736 kb on + strand at 200.736 kb on + strand at 200.737 kb on - strand at 200.737 kb on - strand at 200.737 kb on - strand at 200.740 kb on - strand at 200.740 kb on - strand at 200.740 kb on - strand at 200.740 kb on - strand at 200.740 kb on - strand at 200.740 kb on - strand at 200.839 kb on - strand, within LRK55_RS19275 at 201.019 kb on + strand, within LRK55_RS19275 at 201.019 kb on + strand, within LRK55_RS19275 at 201.019 kb on + strand, within LRK55_RS19275 at 201.020 kb on - strand, within LRK55_RS19275 at 201.020 kb on - strand, within LRK55_RS19275 at 201.020 kb on - strand, within LRK55_RS19275 at 201.036 kb on + strand, within LRK55_RS19275 at 201.036 kb on + strand, within LRK55_RS19275 at 201.037 kb on - strand, within LRK55_RS19275 at 201.092 kb on + strand, within LRK55_RS19275 at 201.093 kb on - strand, within LRK55_RS19275 at 201.105 kb on + strand, within LRK55_RS19275 at 201.237 kb on + strand, within LRK55_RS19275 at 201.237 kb on + strand, within LRK55_RS19275 at 201.237 kb on + strand, within LRK55_RS19275 at 201.237 kb on + strand, within LRK55_RS19275 at 201.237 kb on + strand, within LRK55_RS19275 at 201.238 kb on - strand, within LRK55_RS19275 at 201.238 kb on - strand, within LRK55_RS19275 at 201.306 kb on - strand, within LRK55_RS19275
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc chloride 2560 uM remove 198,796 + LRK55_RS19260 0.78 +0.8 198,797 - LRK55_RS19260 0.78 +0.8 198,797 - LRK55_RS19260 0.78 -0.0 198,797 - LRK55_RS19260 0.78 +0.6 198,797 - LRK55_RS19260 0.78 -2.0 198,797 - LRK55_RS19260 0.78 -1.2 199,003 + LRK55_RS19260 0.87 +0.5 199,004 - LRK55_RS19260 0.87 +0.1 199,315 + -0.1 199,319 + +0.7 199,319 + +0.9 199,320 - +0.4 199,436 + LRK55_RS19265 0.11 +0.6 199,436 + LRK55_RS19265 0.11 +0.3 199,436 + LRK55_RS19265 0.11 +0.0 199,437 - LRK55_RS19265 0.12 +0.0 199,580 + LRK55_RS19265 0.49 +0.0 199,580 + LRK55_RS19265 0.49 -2.2 199,581 - LRK55_RS19265 0.49 -0.5 199,581 - LRK55_RS19265 0.49 +0.1 199,836 + +0.2 199,837 - -0.5 199,837 - -0.2 200,012 + LRK55_RS19270 0.29 +0.9 200,078 - LRK55_RS19270 0.38 +1.6 200,219 + LRK55_RS19270 0.57 -0.1 200,220 - LRK55_RS19270 0.57 +0.2 200,414 + LRK55_RS19270 0.82 +0.2 200,415 - LRK55_RS19270 0.82 +1.4 200,415 - LRK55_RS19270 0.82 -0.5 200,433 + LRK55_RS19270 0.85 -1.2 200,433 + LRK55_RS19270 0.85 -0.2 200,538 + -0.3 200,614 + +0.1 200,614 + +0.4 200,736 + -0.4 200,736 + +3.3 200,737 - -0.5 200,737 - -0.8 200,737 - -1.6 200,740 - -1.2 200,740 - -0.6 200,740 - +1.7 200,740 - -2.1 200,740 - -1.7 200,740 - -0.2 200,839 - LRK55_RS19275 0.16 -0.4 201,019 + LRK55_RS19275 0.30 -2.5 201,019 + LRK55_RS19275 0.30 +0.6 201,019 + LRK55_RS19275 0.30 +0.5 201,020 - LRK55_RS19275 0.30 -1.3 201,020 - LRK55_RS19275 0.30 +0.0 201,020 - LRK55_RS19275 0.30 -0.3 201,036 + LRK55_RS19275 0.31 +1.3 201,036 + LRK55_RS19275 0.31 -0.3 201,037 - LRK55_RS19275 0.32 +0.9 201,092 + LRK55_RS19275 0.36 +0.9 201,093 - LRK55_RS19275 0.36 +0.2 201,105 + LRK55_RS19275 0.37 -0.1 201,237 + LRK55_RS19275 0.47 +0.6 201,237 + LRK55_RS19275 0.47 +0.5 201,237 + LRK55_RS19275 0.47 +0.5 201,237 + LRK55_RS19275 0.47 +1.9 201,237 + LRK55_RS19275 0.47 +0.4 201,238 - LRK55_RS19275 0.47 +1.0 201,238 - LRK55_RS19275 0.47 -1.9 201,306 - LRK55_RS19275 0.53 +0.0
Or see this region's nucleotide sequence