Experiment: R2A with Zinc chloride 2560 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS04085 and LRK55_RS04090 are separated by 108 nucleotides LRK55_RS04090 and LRK55_RS04095 are separated by 5 nucleotides LRK55_RS04095 and LRK55_RS04100 are separated by 15 nucleotides LRK55_RS04100 and LRK55_RS04105 are separated by 103 nucleotides
LRK55_RS04085: LRK55_RS04085 - 3-dehydroquinate synthase, at 866,162 to 867,253
_RS04085
LRK55_RS04090: LRK55_RS04090 - WGR domain-containing protein, at 867,362 to 867,622
_RS04090
LRK55_RS04095: LRK55_RS04095 - uroporphyrinogen decarboxylase, at 867,628 to 868,698
_RS04095
LRK55_RS04100: LRK55_RS04100 - DUF3147 family protein, at 868,714 to 869,067
_RS04100
LRK55_RS04105: LRK55_RS04105 - valine--tRNA ligase, at 869,171 to 871,999
_RS04105
Position (kb) 867
868
869 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 867.225 kb on + strand at 867.310 kb on + strand at 867.310 kb on + strand at 867.312 kb on + strand at 867.312 kb on + strand at 867.313 kb on - strand at 867.313 kb on - strand at 867.313 kb on - strand at 867.371 kb on + strand at 867.372 kb on - strand at 867.410 kb on + strand, within LRK55_RS04090 at 867.411 kb on - strand, within LRK55_RS04090 at 867.459 kb on - strand, within LRK55_RS04090 at 868.735 kb on + strand at 868.735 kb on + strand at 868.735 kb on + strand at 868.735 kb on + strand at 868.735 kb on + strand at 868.735 kb on + strand at 868.736 kb on - strand at 868.736 kb on - strand at 868.736 kb on - strand at 868.897 kb on + strand, within LRK55_RS04100 at 868.897 kb on + strand, within LRK55_RS04100 at 868.898 kb on - strand, within LRK55_RS04100 at 868.898 kb on - strand, within LRK55_RS04100 at 868.898 kb on - strand, within LRK55_RS04100 at 868.898 kb on - strand, within LRK55_RS04100 at 868.898 kb on - strand, within LRK55_RS04100 at 868.909 kb on + strand, within LRK55_RS04100 at 868.909 kb on + strand, within LRK55_RS04100 at 868.909 kb on + strand, within LRK55_RS04100 at 868.909 kb on + strand, within LRK55_RS04100 at 868.909 kb on + strand, within LRK55_RS04100 at 868.910 kb on - strand, within LRK55_RS04100 at 868.910 kb on - strand, within LRK55_RS04100 at 868.977 kb on + strand, within LRK55_RS04100 at 869.037 kb on - strand at 869.037 kb on - strand at 869.037 kb on - strand at 869.107 kb on + strand at 869.108 kb on - strand at 869.108 kb on - strand at 869.108 kb on - strand at 869.128 kb on + strand at 869.128 kb on + strand at 869.128 kb on + strand at 869.128 kb on + strand at 869.129 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc chloride 2560 uM remove 867,225 + -1.9 867,310 + +0.5 867,310 + +1.6 867,312 + -1.4 867,312 + +0.0 867,313 - +0.9 867,313 - -0.3 867,313 - -0.9 867,371 + -0.4 867,372 - -1.7 867,410 + LRK55_RS04090 0.18 -0.8 867,411 - LRK55_RS04090 0.19 -0.1 867,459 - LRK55_RS04090 0.37 -0.6 868,735 + +0.3 868,735 + +2.1 868,735 + -0.2 868,735 + -0.1 868,735 + -2.3 868,735 + +1.2 868,736 - +0.5 868,736 - -1.9 868,736 - -3.0 868,897 + LRK55_RS04100 0.52 +0.0 868,897 + LRK55_RS04100 0.52 +0.5 868,898 - LRK55_RS04100 0.52 +0.3 868,898 - LRK55_RS04100 0.52 -0.1 868,898 - LRK55_RS04100 0.52 -2.1 868,898 - LRK55_RS04100 0.52 +0.1 868,898 - LRK55_RS04100 0.52 -2.4 868,909 + LRK55_RS04100 0.55 -2.5 868,909 + LRK55_RS04100 0.55 +0.6 868,909 + LRK55_RS04100 0.55 +0.7 868,909 + LRK55_RS04100 0.55 -0.6 868,909 + LRK55_RS04100 0.55 -1.1 868,910 - LRK55_RS04100 0.55 +0.6 868,910 - LRK55_RS04100 0.55 +0.1 868,977 + LRK55_RS04100 0.74 -0.1 869,037 - -0.1 869,037 - +1.4 869,037 - -0.2 869,107 + +0.6 869,108 - -1.3 869,108 - -0.9 869,108 - +0.6 869,128 + -0.4 869,128 + +0.3 869,128 + -0.3 869,128 + -0.3 869,129 - -0.7
Or see this region's nucleotide sequence