Experiment: R2A with Zinc chloride 2560 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt LRK55_RS09995 and LRK55_RS10000 are separated by 120 nucleotides LRK55_RS10000 and LRK55_RS10005 overlap by 4 nucleotides
LRK55_RS09995: LRK55_RS09995 - threonine synthase, at 2,192,596 to 2,193,906
_RS09995
LRK55_RS10000: LRK55_RS10000 - homoserine kinase, at 2,194,027 to 2,195,022
_RS10000
LRK55_RS10005: LRK55_RS10005 - homoserine dehydrogenase, at 2,195,019 to 2,196,164
_RS10005
Position (kb)
2194
2195
2196 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 2193.212 kb on + strand, within LRK55_RS09995 at 2193.215 kb on + strand, within LRK55_RS09995 at 2193.215 kb on + strand, within LRK55_RS09995 at 2193.216 kb on - strand, within LRK55_RS09995 at 2193.216 kb on - strand, within LRK55_RS09995 at 2193.216 kb on - strand, within LRK55_RS09995 at 2193.216 kb on - strand, within LRK55_RS09995 at 2193.216 kb on - strand, within LRK55_RS09995 at 2193.420 kb on - strand, within LRK55_RS09995 at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.806 kb on + strand at 2193.807 kb on - strand at 2193.807 kb on - strand at 2193.807 kb on - strand at 2193.807 kb on - strand at 2194.173 kb on - strand, within LRK55_RS10000 at 2194.173 kb on - strand, within LRK55_RS10000 at 2195.123 kb on + strand at 2195.214 kb on + strand, within LRK55_RS10005 at 2195.214 kb on + strand, within LRK55_RS10005 at 2195.215 kb on - strand, within LRK55_RS10005 at 2195.486 kb on + strand, within LRK55_RS10005 at 2195.488 kb on + strand, within LRK55_RS10005 at 2195.488 kb on + strand, within LRK55_RS10005 at 2195.488 kb on + strand, within LRK55_RS10005 at 2195.489 kb on - strand, within LRK55_RS10005 at 2195.489 kb on - strand, within LRK55_RS10005 at 2195.489 kb on - strand, within LRK55_RS10005
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Zinc chloride 2560 uM remove 2,193,212 + LRK55_RS09995 0.47 -4.5 2,193,215 + LRK55_RS09995 0.47 -0.7 2,193,215 + LRK55_RS09995 0.47 -1.4 2,193,216 - LRK55_RS09995 0.47 -2.1 2,193,216 - LRK55_RS09995 0.47 -1.6 2,193,216 - LRK55_RS09995 0.47 -3.4 2,193,216 - LRK55_RS09995 0.47 -0.7 2,193,216 - LRK55_RS09995 0.47 -0.8 2,193,420 - LRK55_RS09995 0.63 -0.5 2,193,806 + -0.5 2,193,806 + +0.3 2,193,806 + +0.9 2,193,806 + -1.1 2,193,806 + -1.2 2,193,806 + -2.2 2,193,806 + -0.4 2,193,806 + +0.3 2,193,806 + -2.2 2,193,807 - +0.1 2,193,807 - +0.4 2,193,807 - -1.7 2,193,807 - -3.6 2,194,173 - LRK55_RS10000 0.15 -1.1 2,194,173 - LRK55_RS10000 0.15 -2.3 2,195,123 + +0.1 2,195,214 + LRK55_RS10005 0.17 +1.4 2,195,214 + LRK55_RS10005 0.17 +0.1 2,195,215 - LRK55_RS10005 0.17 -0.6 2,195,486 + LRK55_RS10005 0.41 +0.4 2,195,488 + LRK55_RS10005 0.41 +0.8 2,195,488 + LRK55_RS10005 0.41 +0.6 2,195,488 + LRK55_RS10005 0.41 -1.8 2,195,489 - LRK55_RS10005 0.41 +0.3 2,195,489 - LRK55_RS10005 0.41 -0.1 2,195,489 - LRK55_RS10005 0.41 +0.1
Or see this region's nucleotide sequence