Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS21830

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS21825 and ECOLIN_RS21830 are separated by 21 nucleotidesECOLIN_RS21830 and ECOLIN_RS21835 overlap by 4 nucleotides ECOLIN_RS21825: ECOLIN_RS21825 - uroporphyrinogen-III C-methyltransferase, at 4,384,679 to 4,385,896 _RS21825 ECOLIN_RS21830: ECOLIN_RS21830 - uroporphyrinogen-III synthase, at 4,385,918 to 4,386,658 _RS21830 ECOLIN_RS21835: ECOLIN_RS21835 - hydroxymethylbilane synthase, at 4,386,655 to 4,387,596 _RS21835 Position (kb) 4385 4386 4387Strain fitness (log2 ratio) -1 0 1at 4384.947 kb on + strand, within ECOLIN_RS21825at 4384.948 kb on - strand, within ECOLIN_RS21825at 4384.951 kb on - strand, within ECOLIN_RS21825at 4384.951 kb on - strand, within ECOLIN_RS21825at 4384.958 kb on + strand, within ECOLIN_RS21825at 4384.992 kb on + strand, within ECOLIN_RS21825at 4384.993 kb on - strand, within ECOLIN_RS21825at 4385.064 kb on + strand, within ECOLIN_RS21825at 4385.064 kb on + strand, within ECOLIN_RS21825at 4385.065 kb on - strand, within ECOLIN_RS21825at 4385.123 kb on - strand, within ECOLIN_RS21825at 4385.910 kb on + strandat 4385.920 kb on - strandat 4385.924 kb on + strandat 4385.925 kb on - strandat 4386.678 kb on - strandat 4386.678 kb on - strandat 4387.613 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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4,384,947 + ECOLIN_RS21825 0.22 -0.8
4,384,948 - ECOLIN_RS21825 0.22 -0.1
4,384,951 - ECOLIN_RS21825 0.22 -0.5
4,384,951 - ECOLIN_RS21825 0.22 +0.2
4,384,958 + ECOLIN_RS21825 0.23 -0.0
4,384,992 + ECOLIN_RS21825 0.26 +1.2
4,384,993 - ECOLIN_RS21825 0.26 -0.2
4,385,064 + ECOLIN_RS21825 0.32 +0.0
4,385,064 + ECOLIN_RS21825 0.32 +0.1
4,385,065 - ECOLIN_RS21825 0.32 +0.0
4,385,123 - ECOLIN_RS21825 0.36 -0.3
4,385,910 + -0.0
4,385,920 - +0.0
4,385,924 + -0.8
4,385,925 - +0.2
4,386,678 - +0.1
4,386,678 - -0.4
4,387,613 - -0.2

Or see this region's nucleotide sequence