Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS16175

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS16165 and ECOLIN_RS16170 are separated by 117 nucleotidesECOLIN_RS16170 and ECOLIN_RS16175 are separated by 23 nucleotidesECOLIN_RS16175 and ECOLIN_RS31590 are separated by 290 nucleotidesECOLIN_RS31590 and ECOLIN_RS16180 are separated by 73 nucleotides ECOLIN_RS16165: ECOLIN_RS16165 - aminomethyl-transferring glycine dehydrogenase, at 3,277,752 to 3,280,625 _RS16165 ECOLIN_RS16170: ECOLIN_RS16170 - glycine cleavage system protein GcvH, at 3,280,743 to 3,281,132 _RS16170 ECOLIN_RS16175: ECOLIN_RS16175 - glycine cleavage system aminomethyltransferase GcvT, at 3,281,156 to 3,282,250 _RS16175 ECOLIN_RS31590: ECOLIN_RS31590 - hypothetical protein, at 3,282,541 to 3,282,624 _RS31590 ECOLIN_RS16180: ECOLIN_RS16180 - FAD-dependent 2-octaprenylphenol hydroxylase, at 3,282,698 to 3,283,900 _RS16180 Position (kb) 3281 3282 3283Strain fitness (log2 ratio) -2 -1 0 1at 3280.301 kb on - strand, within ECOLIN_RS16165at 3280.326 kb on - strand, within ECOLIN_RS16165at 3280.326 kb on - strand, within ECOLIN_RS16165at 3280.375 kb on + strandat 3280.583 kb on + strandat 3280.612 kb on - strandat 3280.756 kb on - strandat 3280.756 kb on - strandat 3280.844 kb on - strand, within ECOLIN_RS16170at 3281.060 kb on - strand, within ECOLIN_RS16170at 3281.079 kb on - strand, within ECOLIN_RS16170at 3281.105 kb on - strandat 3281.122 kb on - strandat 3281.204 kb on - strandat 3281.252 kb on - strandat 3281.281 kb on + strand, within ECOLIN_RS16175at 3281.281 kb on + strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.282 kb on - strand, within ECOLIN_RS16175at 3281.306 kb on - strand, within ECOLIN_RS16175at 3281.544 kb on + strand, within ECOLIN_RS16175at 3282.098 kb on - strand, within ECOLIN_RS16175at 3282.127 kb on + strand, within ECOLIN_RS16175at 3282.696 kb on - strandat 3282.696 kb on - strandat 3282.700 kb on - strandat 3282.854 kb on - strand, within ECOLIN_RS16180at 3282.995 kb on + strand, within ECOLIN_RS16180at 3283.125 kb on - strand, within ECOLIN_RS16180

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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3,280,301 - ECOLIN_RS16165 0.89 -0.1
3,280,326 - ECOLIN_RS16165 0.90 -0.3
3,280,326 - ECOLIN_RS16165 0.90 -0.6
3,280,375 + -2.5
3,280,583 + -0.4
3,280,612 - +0.1
3,280,756 - -0.3
3,280,756 - +0.3
3,280,844 - ECOLIN_RS16170 0.26 -0.4
3,281,060 - ECOLIN_RS16170 0.81 -0.7
3,281,079 - ECOLIN_RS16170 0.86 -0.6
3,281,105 - -0.1
3,281,122 - +0.1
3,281,204 - -0.3
3,281,252 - -0.3
3,281,281 + ECOLIN_RS16175 0.11 -0.1
3,281,281 + ECOLIN_RS16175 0.11 +1.5
3,281,282 - ECOLIN_RS16175 0.12 -0.6
3,281,282 - ECOLIN_RS16175 0.12 -0.4
3,281,282 - ECOLIN_RS16175 0.12 -1.9
3,281,282 - ECOLIN_RS16175 0.12 -0.5
3,281,282 - ECOLIN_RS16175 0.12 -0.8
3,281,306 - ECOLIN_RS16175 0.14 -1.2
3,281,544 + ECOLIN_RS16175 0.35 -0.1
3,282,098 - ECOLIN_RS16175 0.86 -0.5
3,282,127 + ECOLIN_RS16175 0.89 -0.6
3,282,696 - -0.3
3,282,696 - +1.7
3,282,700 - +0.3
3,282,854 - ECOLIN_RS16180 0.13 -1.5
3,282,995 + ECOLIN_RS16180 0.25 -2.7
3,283,125 - ECOLIN_RS16180 0.35 -2.5

Or see this region's nucleotide sequence