Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS10680

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS10665 and ECOLIN_RS10670 are separated by 21 nucleotidesECOLIN_RS10670 and ECOLIN_RS10675 are separated by 40 nucleotidesECOLIN_RS10675 and ECOLIN_RS10680 are separated by 58 nucleotidesECOLIN_RS10680 and ECOLIN_RS10685 overlap by 22 nucleotidesECOLIN_RS10685 and ECOLIN_RS10690 are separated by 52 nucleotides ECOLIN_RS10665: ECOLIN_RS10665 - DUF4222 domain-containing protein, at 2,076,683 to 2,076,937 _RS10665 ECOLIN_RS10670: ECOLIN_RS10670 - 3'-5' exonuclease, at 2,076,959 to 2,077,528 _RS10670 ECOLIN_RS10675: ECOLIN_RS10675 - excisionase family protein, at 2,077,569 to 2,077,805 _RS10675 ECOLIN_RS10680: ECOLIN_RS10680 - site-specific integrase, at 2,077,864 to 2,079,177 _RS10680 ECOLIN_RS10685: ECOLIN_RS10685 - DUF72 domain-containing protein, at 2,079,156 to 2,079,929 _RS10685 ECOLIN_RS10690: ECOLIN_RS10690 - MAPEG family protein, at 2,079,982 to 2,080,377 _RS10690 Position (kb) 2077 2078 2079 2080Strain fitness (log2 ratio) -1 0 1at 2077.260 kb on - strand, within ECOLIN_RS10670at 2077.762 kb on + strand, within ECOLIN_RS10675at 2079.197 kb on + strandat 2079.725 kb on + strand, within ECOLIN_RS10685

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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2,077,260 - ECOLIN_RS10670 0.53 +0.2
2,077,762 + ECOLIN_RS10675 0.81 +0.2
2,079,197 + -0.8
2,079,725 + ECOLIN_RS10685 0.74 +1.0

Or see this region's nucleotide sequence