Strain Fitness in Escherichia coli Nissle 1917 around ECOLIN_RS04300

Experiment: Casamino-acids

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntECOLIN_RS04295 and ECOLIN_RS04300 overlap by 4 nucleotidesECOLIN_RS04300 and ECOLIN_RS04310 are separated by 116 nucleotidesECOLIN_RS04310 and ECOLIN_RS04315 overlap by 4 nucleotides ECOLIN_RS04295: ECOLIN_RS04295 - 23S rRNA (adenine(1618)-N(6))-methyltransferase RlmF, at 869,936 to 870,862 _RS04295 ECOLIN_RS04300: ECOLIN_RS04300 - mechanosensitive channel protein, at 870,859 to 873,084 _RS04300 ECOLIN_RS04310: ECOLIN_RS04310 - glutamine ABC transporter ATP-binding protein GlnQ, at 873,201 to 873,923 _RS04310 ECOLIN_RS04315: ECOLIN_RS04315 - glutamine ABC transporter permease GlnP, at 873,920 to 874,579 _RS04315 Position (kb) 870 871 872 873 874Strain fitness (log2 ratio) -1 0 1at 869.881 kb on - strandat 870.193 kb on + strand, within ECOLIN_RS04295at 870.538 kb on + strand, within ECOLIN_RS04295at 870.804 kb on + strandat 871.193 kb on + strand, within ECOLIN_RS04300at 871.445 kb on - strand, within ECOLIN_RS04300at 871.968 kb on + strand, within ECOLIN_RS04300at 873.912 kb on - strandat 873.959 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Casamino-acids
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869,881 - +0.3
870,193 + ECOLIN_RS04295 0.28 +0.1
870,538 + ECOLIN_RS04295 0.65 +0.5
870,804 + -0.7
871,193 + ECOLIN_RS04300 0.15 -0.3
871,445 - ECOLIN_RS04300 0.26 +0.0
871,968 + ECOLIN_RS04300 0.50 +0.1
873,912 - -1.0
873,959 - +0.2

Or see this region's nucleotide sequence