Experiment: Casamino-acids
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ECOLIN_RS00370 and ECOLIN_RS00375 are separated by 163 nucleotides ECOLIN_RS00375 and ECOLIN_RS00380 are separated by 88 nucleotides ECOLIN_RS00380 and ECOLIN_RS00385 are separated by 125 nucleotides ECOLIN_RS00385 and ECOLIN_RS00390 are separated by 10 nucleotides
ECOLIN_RS00370: ECOLIN_RS00370 - thiamine ABC transporter substrate binding subunit, at 77,477 to 78,460
_RS00370
ECOLIN_RS00375: ECOLIN_RS00375 - DNA-binding transcriptional regulator SgrR, at 78,624 to 80,279
_RS00375
ECOLIN_RS00380: ECOLIN_RS00380 - glucose uptake inhibitor SgrT, at 80,368 to 80,499
_RS00380
ECOLIN_RS00385: ECOLIN_RS00385 - 3-isopropylmalate dehydratase small subunit, at 80,625 to 81,230
_RS00385
ECOLIN_RS00390: ECOLIN_RS00390 - 3-isopropylmalate dehydratase large subunit, at 81,241 to 82,641
_RS00390
Position (kb) 78
79
80
81 Strain fitness (log2 ratio) -2
-1
0
1 at 77.769 kb on + strand, within ECOLIN_RS00370 at 77.774 kb on - strand, within ECOLIN_RS00370 at 77.818 kb on - strand, within ECOLIN_RS00370 at 77.978 kb on - strand, within ECOLIN_RS00370 at 78.381 kb on + strand at 78.381 kb on + strand at 78.382 kb on - strand at 78.382 kb on - strand at 78.385 kb on + strand at 78.386 kb on - strand at 78.387 kb on + strand at 78.388 kb on - strand at 78.600 kb on - strand at 78.701 kb on + strand at 78.701 kb on + strand at 78.702 kb on - strand at 78.702 kb on - strand at 78.702 kb on - strand at 78.702 kb on - strand at 78.702 kb on - strand at 78.702 kb on - strand at 79.271 kb on - strand, within ECOLIN_RS00375 at 79.274 kb on - strand, within ECOLIN_RS00375 at 79.809 kb on + strand, within ECOLIN_RS00375 at 80.163 kb on - strand at 80.222 kb on + strand at 80.222 kb on + strand at 80.325 kb on + strand at 80.328 kb on + strand at 80.329 kb on - strand at 80.656 kb on + strand at 80.667 kb on - strand at 80.745 kb on - strand, within ECOLIN_RS00385 at 80.793 kb on + strand, within ECOLIN_RS00385 at 81.243 kb on - strand at 81.246 kb on + strand at 81.246 kb on + strand at 81.247 kb on - strand at 81.247 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Casamino-acids remove 77,769 + ECOLIN_RS00370 0.30 -0.4 77,774 - ECOLIN_RS00370 0.30 -0.3 77,818 - ECOLIN_RS00370 0.35 -0.4 77,978 - ECOLIN_RS00370 0.51 -0.3 78,381 + -0.2 78,381 + +1.1 78,382 - -0.3 78,382 - -0.0 78,385 + +0.3 78,386 - +0.1 78,387 + +0.4 78,388 - +0.2 78,600 - -0.4 78,701 + -2.3 78,701 + +1.1 78,702 - +0.0 78,702 - -0.6 78,702 - +0.4 78,702 - +0.4 78,702 - +0.5 78,702 - -0.3 79,271 - ECOLIN_RS00375 0.39 -0.2 79,274 - ECOLIN_RS00375 0.39 -0.3 79,809 + ECOLIN_RS00375 0.72 -0.7 80,163 - -0.0 80,222 + +0.8 80,222 + -0.7 80,325 + -0.2 80,328 + -0.8 80,329 - +0.2 80,656 + -0.4 80,667 - +1.1 80,745 - ECOLIN_RS00385 0.20 +1.1 80,793 + ECOLIN_RS00385 0.28 +0.3 81,243 - -0.3 81,246 + +0.2 81,246 + +0.4 81,247 - -0.8 81,247 - -0.0
Or see this region's nucleotide sequence