Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMJJ_RS08695 and MMJJ_RS08700 are separated by 70 nucleotides MMJJ_RS08700 and MMJJ_RS08705 overlap by 4 nucleotides MMJJ_RS08705 and MMJJ_RS08710 are separated by 73 nucleotides MMJJ_RS08710 and MMJJ_RS08715 are separated by 33 nucleotides
MMJJ_RS08695: MMJJ_RS08695 - phosphoenolpyruvate synthase, at 1,599,615 to 1,601,891
_RS08695
MMJJ_RS08700: MMJJ_RS08700 - phosphopantetheine adenylyltransferase, at 1,601,962 to 1,602,408
_RS08700
MMJJ_RS08705: MMJJ_RS08705 - AEC family transporter, at 1,602,405 to 1,603,319
_RS08705
MMJJ_RS08710: MMJJ_RS08710 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 1,603,393 to 1,604,241
_RS08710
MMJJ_RS08715: MMJJ_RS08715 - NAD-dependent epimerase/dehydratase family protein, at 1,604,275 to 1,605,150
_RS08715
Position (kb)
1602
1603
1604 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1602.385 kb on + strand at 1602.409 kb on - strand at 1602.409 kb on - strand at 1602.426 kb on - strand at 1602.426 kb on - strand at 1602.426 kb on - strand at 1602.426 kb on - strand at 1602.426 kb on - strand at 1602.426 kb on - strand at 1602.427 kb on + strand at 1602.427 kb on + strand at 1602.427 kb on + strand at 1602.427 kb on + strand at 1602.427 kb on + strand at 1602.459 kb on + strand at 1602.556 kb on - strand, within MMJJ_RS08705 at 1602.560 kb on - strand, within MMJJ_RS08705 at 1602.560 kb on - strand, within MMJJ_RS08705 at 1602.561 kb on + strand, within MMJJ_RS08705 at 1602.564 kb on + strand, within MMJJ_RS08705 at 1602.568 kb on - strand, within MMJJ_RS08705 at 1602.569 kb on + strand, within MMJJ_RS08705 at 1602.610 kb on - strand, within MMJJ_RS08705 at 1602.613 kb on - strand, within MMJJ_RS08705 at 1602.613 kb on - strand, within MMJJ_RS08705 at 1602.648 kb on - strand, within MMJJ_RS08705 at 1602.648 kb on - strand, within MMJJ_RS08705 at 1602.649 kb on + strand, within MMJJ_RS08705 at 1602.650 kb on - strand, within MMJJ_RS08705 at 1602.650 kb on - strand, within MMJJ_RS08705 at 1602.650 kb on - strand, within MMJJ_RS08705 at 1602.651 kb on + strand, within MMJJ_RS08705 at 1602.651 kb on + strand, within MMJJ_RS08705 at 1602.701 kb on + strand, within MMJJ_RS08705 at 1602.834 kb on - strand, within MMJJ_RS08705 at 1602.834 kb on - strand, within MMJJ_RS08705 at 1602.834 kb on - strand, within MMJJ_RS08705 at 1602.835 kb on + strand, within MMJJ_RS08705 at 1602.835 kb on + strand, within MMJJ_RS08705 at 1602.835 kb on + strand, within MMJJ_RS08705 at 1602.914 kb on + strand, within MMJJ_RS08705 at 1602.931 kb on - strand, within MMJJ_RS08705 at 1603.016 kb on + strand, within MMJJ_RS08705 at 1603.090 kb on - strand, within MMJJ_RS08705 at 1603.103 kb on + strand, within MMJJ_RS08705 at 1603.157 kb on + strand, within MMJJ_RS08705 at 1603.160 kb on - strand, within MMJJ_RS08705 at 1603.210 kb on - strand, within MMJJ_RS08705 at 1603.210 kb on - strand, within MMJJ_RS08705 at 1603.240 kb on - strand at 1603.279 kb on - strand at 1603.280 kb on + strand at 1603.280 kb on + strand at 1603.280 kb on + strand at 1603.301 kb on + strand at 1603.301 kb on + strand at 1603.305 kb on - strand at 1603.306 kb on + strand at 1603.312 kb on - strand at 1603.330 kb on - strand at 1603.345 kb on - strand at 1603.345 kb on - strand at 1603.363 kb on - strand at 1603.363 kb on - strand at 1603.364 kb on + strand at 1603.364 kb on + strand at 1603.366 kb on - strand at 1603.366 kb on - strand at 1603.367 kb on + strand at 1603.370 kb on + strand at 1603.370 kb on + strand at 1603.372 kb on + strand at 1603.392 kb on + strand at 1603.412 kb on - strand at 1603.499 kb on - strand, within MMJJ_RS08710 at 1603.500 kb on + strand, within MMJJ_RS08710 at 1603.500 kb on + strand, within MMJJ_RS08710 at 1603.500 kb on + strand, within MMJJ_RS08710 at 1603.504 kb on - strand, within MMJJ_RS08710 at 1603.504 kb on - strand, within MMJJ_RS08710 at 1603.504 kb on - strand, within MMJJ_RS08710 at 1603.505 kb on + strand, within MMJJ_RS08710 at 1603.509 kb on + strand, within MMJJ_RS08710 at 1603.548 kb on + strand, within MMJJ_RS08710 at 1603.551 kb on + strand, within MMJJ_RS08710 at 1603.555 kb on + strand, within MMJJ_RS08710 at 1603.662 kb on - strand, within MMJJ_RS08710 at 1603.663 kb on + strand, within MMJJ_RS08710 at 1603.665 kb on - strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.681 kb on + strand, within MMJJ_RS08710 at 1603.682 kb on - strand, within MMJJ_RS08710 at 1603.683 kb on + strand, within MMJJ_RS08710 at 1603.683 kb on + strand, within MMJJ_RS08710 at 1603.684 kb on - strand, within MMJJ_RS08710 at 1603.684 kb on - strand, within MMJJ_RS08710 at 1603.688 kb on + strand, within MMJJ_RS08710 at 1603.688 kb on + strand, within MMJJ_RS08710 at 1603.728 kb on + strand, within MMJJ_RS08710 at 1603.766 kb on - strand, within MMJJ_RS08710 at 1603.766 kb on - strand, within MMJJ_RS08710 at 1603.770 kb on + strand, within MMJJ_RS08710 at 1603.884 kb on - strand, within MMJJ_RS08710 at 1603.899 kb on + strand, within MMJJ_RS08710 at 1603.961 kb on - strand, within MMJJ_RS08710 at 1603.985 kb on - strand, within MMJJ_RS08710 at 1604.000 kb on + strand, within MMJJ_RS08710 at 1604.004 kb on + strand, within MMJJ_RS08710 at 1604.070 kb on - strand, within MMJJ_RS08710 at 1604.142 kb on - strand, within MMJJ_RS08710 at 1604.196 kb on - strand at 1604.196 kb on - strand at 1604.197 kb on + strand at 1604.197 kb on + strand at 1604.198 kb on - strand at 1604.199 kb on + strand at 1604.199 kb on + strand at 1604.199 kb on + strand at 1604.216 kb on + strand at 1604.246 kb on + strand at 1604.247 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.251 kb on - strand at 1604.252 kb on + strand at 1604.252 kb on + strand at 1604.254 kb on - strand at 1604.254 kb on - strand at 1604.255 kb on + strand at 1604.259 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE remove 1,602,385 + -1.1 1,602,409 - +0.5 1,602,409 - -1.0 1,602,426 - +0.1 1,602,426 - -1.2 1,602,426 - -0.7 1,602,426 - +1.9 1,602,426 - -1.3 1,602,426 - +0.9 1,602,427 + +1.6 1,602,427 + -0.5 1,602,427 + +0.8 1,602,427 + +0.6 1,602,427 + +0.8 1,602,459 + +1.0 1,602,556 - MMJJ_RS08705 0.17 +1.4 1,602,560 - MMJJ_RS08705 0.17 +0.6 1,602,560 - MMJJ_RS08705 0.17 +0.0 1,602,561 + MMJJ_RS08705 0.17 +1.5 1,602,564 + MMJJ_RS08705 0.17 +1.0 1,602,568 - MMJJ_RS08705 0.18 +0.9 1,602,569 + MMJJ_RS08705 0.18 +0.8 1,602,610 - MMJJ_RS08705 0.22 +0.3 1,602,613 - MMJJ_RS08705 0.23 +2.0 1,602,613 - MMJJ_RS08705 0.23 +1.2 1,602,648 - MMJJ_RS08705 0.27 +0.8 1,602,648 - MMJJ_RS08705 0.27 +0.5 1,602,649 + MMJJ_RS08705 0.27 -1.7 1,602,650 - MMJJ_RS08705 0.27 +1.2 1,602,650 - MMJJ_RS08705 0.27 -0.9 1,602,650 - MMJJ_RS08705 0.27 +2.3 1,602,651 + MMJJ_RS08705 0.27 -2.1 1,602,651 + MMJJ_RS08705 0.27 +1.2 1,602,701 + MMJJ_RS08705 0.32 -1.7 1,602,834 - MMJJ_RS08705 0.47 +0.9 1,602,834 - MMJJ_RS08705 0.47 +0.3 1,602,834 - MMJJ_RS08705 0.47 +0.2 1,602,835 + MMJJ_RS08705 0.47 +0.8 1,602,835 + MMJJ_RS08705 0.47 +0.8 1,602,835 + MMJJ_RS08705 0.47 +0.9 1,602,914 + MMJJ_RS08705 0.56 -1.2 1,602,931 - MMJJ_RS08705 0.57 +0.4 1,603,016 + MMJJ_RS08705 0.67 +2.1 1,603,090 - MMJJ_RS08705 0.75 +0.7 1,603,103 + MMJJ_RS08705 0.76 +1.2 1,603,157 + MMJJ_RS08705 0.82 -0.7 1,603,160 - MMJJ_RS08705 0.83 -1.0 1,603,210 - MMJJ_RS08705 0.88 +0.5 1,603,210 - MMJJ_RS08705 0.88 +1.7 1,603,240 - +1.1 1,603,279 - -1.3 1,603,280 + +0.7 1,603,280 + -0.6 1,603,280 + +0.1 1,603,301 + -0.7 1,603,301 + -1.0 1,603,305 - +0.9 1,603,306 + -1.1 1,603,312 - -1.3 1,603,330 - -0.1 1,603,345 - +0.9 1,603,345 - -0.5 1,603,363 - -1.3 1,603,363 - -0.7 1,603,364 + -0.2 1,603,364 + +2.0 1,603,366 - +1.2 1,603,366 - +1.3 1,603,367 + -2.0 1,603,370 + +1.0 1,603,370 + +1.5 1,603,372 + -1.1 1,603,392 + +1.3 1,603,412 - -1.3 1,603,499 - MMJJ_RS08710 0.12 -0.9 1,603,500 + MMJJ_RS08710 0.13 +1.5 1,603,500 + MMJJ_RS08710 0.13 -0.9 1,603,500 + MMJJ_RS08710 0.13 -1.5 1,603,504 - MMJJ_RS08710 0.13 -0.7 1,603,504 - MMJJ_RS08710 0.13 -3.2 1,603,504 - MMJJ_RS08710 0.13 +0.0 1,603,505 + MMJJ_RS08710 0.13 -0.9 1,603,509 + MMJJ_RS08710 0.14 -0.3 1,603,548 + MMJJ_RS08710 0.18 -0.9 1,603,551 + MMJJ_RS08710 0.19 -1.5 1,603,555 + MMJJ_RS08710 0.19 -0.8 1,603,662 - MMJJ_RS08710 0.32 -1.4 1,603,663 + MMJJ_RS08710 0.32 -0.9 1,603,665 - MMJJ_RS08710 0.32 -2.3 1,603,681 + MMJJ_RS08710 0.34 -0.7 1,603,681 + MMJJ_RS08710 0.34 +0.1 1,603,681 + MMJJ_RS08710 0.34 +1.0 1,603,681 + MMJJ_RS08710 0.34 +1.4 1,603,682 - MMJJ_RS08710 0.34 +0.2 1,603,683 + MMJJ_RS08710 0.34 -0.5 1,603,683 + MMJJ_RS08710 0.34 +1.5 1,603,684 - MMJJ_RS08710 0.34 +0.2 1,603,684 - MMJJ_RS08710 0.34 -0.8 1,603,688 + MMJJ_RS08710 0.35 -0.9 1,603,688 + MMJJ_RS08710 0.35 +1.0 1,603,728 + MMJJ_RS08710 0.39 -0.3 1,603,766 - MMJJ_RS08710 0.44 +3.0 1,603,766 - MMJJ_RS08710 0.44 -0.3 1,603,770 + MMJJ_RS08710 0.44 -0.5 1,603,884 - MMJJ_RS08710 0.58 -0.7 1,603,899 + MMJJ_RS08710 0.60 -0.2 1,603,961 - MMJJ_RS08710 0.67 +0.0 1,603,985 - MMJJ_RS08710 0.70 +0.2 1,604,000 + MMJJ_RS08710 0.71 -1.1 1,604,004 + MMJJ_RS08710 0.72 -0.1 1,604,070 - MMJJ_RS08710 0.80 -2.2 1,604,142 - MMJJ_RS08710 0.88 -1.3 1,604,196 - +0.5 1,604,196 - -0.5 1,604,197 + -0.6 1,604,197 + -0.1 1,604,198 - -0.1 1,604,199 + +0.0 1,604,199 + +2.0 1,604,199 + -0.3 1,604,216 + -0.3 1,604,246 + -1.4 1,604,247 - -1.9 1,604,251 - -0.2 1,604,251 - +0.2 1,604,251 - -1.1 1,604,251 - -0.3 1,604,251 - -1.2 1,604,252 + -1.3 1,604,252 + +0.5 1,604,254 - -1.3 1,604,254 - -1.3 1,604,255 + +0.5 1,604,259 - -1.1
Or see this region's nucleotide sequence