Strain Fitness in Methanococcus maripaludis JJ around MMJJ_RS08705

Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntMMJJ_RS08695 and MMJJ_RS08700 are separated by 70 nucleotidesMMJJ_RS08700 and MMJJ_RS08705 overlap by 4 nucleotidesMMJJ_RS08705 and MMJJ_RS08710 are separated by 73 nucleotidesMMJJ_RS08710 and MMJJ_RS08715 are separated by 33 nucleotides MMJJ_RS08695: MMJJ_RS08695 - phosphoenolpyruvate synthase, at 1,599,615 to 1,601,891 _RS08695 MMJJ_RS08700: MMJJ_RS08700 - phosphopantetheine adenylyltransferase, at 1,601,962 to 1,602,408 _RS08700 MMJJ_RS08705: MMJJ_RS08705 - AEC family transporter, at 1,602,405 to 1,603,319 _RS08705 MMJJ_RS08710: MMJJ_RS08710 - UTP--glucose-1-phosphate uridylyltransferase GalU, at 1,603,393 to 1,604,241 _RS08710 MMJJ_RS08715: MMJJ_RS08715 - NAD-dependent epimerase/dehydratase family protein, at 1,604,275 to 1,605,150 _RS08715 Position (kb) 1602 1603 1604Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1602.385 kb on + strandat 1602.409 kb on - strandat 1602.409 kb on - strandat 1602.426 kb on - strandat 1602.426 kb on - strandat 1602.426 kb on - strandat 1602.426 kb on - strandat 1602.426 kb on - strandat 1602.426 kb on - strandat 1602.427 kb on + strandat 1602.427 kb on + strandat 1602.427 kb on + strandat 1602.427 kb on + strandat 1602.427 kb on + strandat 1602.459 kb on + strandat 1602.556 kb on - strand, within MMJJ_RS08705at 1602.560 kb on - strand, within MMJJ_RS08705at 1602.560 kb on - strand, within MMJJ_RS08705at 1602.561 kb on + strand, within MMJJ_RS08705at 1602.564 kb on + strand, within MMJJ_RS08705at 1602.568 kb on - strand, within MMJJ_RS08705at 1602.569 kb on + strand, within MMJJ_RS08705at 1602.610 kb on - strand, within MMJJ_RS08705at 1602.613 kb on - strand, within MMJJ_RS08705at 1602.613 kb on - strand, within MMJJ_RS08705at 1602.648 kb on - strand, within MMJJ_RS08705at 1602.648 kb on - strand, within MMJJ_RS08705at 1602.649 kb on + strand, within MMJJ_RS08705at 1602.650 kb on - strand, within MMJJ_RS08705at 1602.650 kb on - strand, within MMJJ_RS08705at 1602.650 kb on - strand, within MMJJ_RS08705at 1602.651 kb on + strand, within MMJJ_RS08705at 1602.651 kb on + strand, within MMJJ_RS08705at 1602.701 kb on + strand, within MMJJ_RS08705at 1602.834 kb on - strand, within MMJJ_RS08705at 1602.834 kb on - strand, within MMJJ_RS08705at 1602.834 kb on - strand, within MMJJ_RS08705at 1602.835 kb on + strand, within MMJJ_RS08705at 1602.835 kb on + strand, within MMJJ_RS08705at 1602.835 kb on + strand, within MMJJ_RS08705at 1602.914 kb on + strand, within MMJJ_RS08705at 1602.931 kb on - strand, within MMJJ_RS08705at 1603.016 kb on + strand, within MMJJ_RS08705at 1603.090 kb on - strand, within MMJJ_RS08705at 1603.103 kb on + strand, within MMJJ_RS08705at 1603.157 kb on + strand, within MMJJ_RS08705at 1603.160 kb on - strand, within MMJJ_RS08705at 1603.210 kb on - strand, within MMJJ_RS08705at 1603.210 kb on - strand, within MMJJ_RS08705at 1603.240 kb on - strandat 1603.279 kb on - strandat 1603.280 kb on + strandat 1603.280 kb on + strandat 1603.280 kb on + strandat 1603.301 kb on + strandat 1603.301 kb on + strandat 1603.305 kb on - strandat 1603.306 kb on + strandat 1603.312 kb on - strandat 1603.330 kb on - strandat 1603.345 kb on - strandat 1603.345 kb on - strandat 1603.363 kb on - strandat 1603.363 kb on - strandat 1603.364 kb on + strandat 1603.364 kb on + strandat 1603.366 kb on - strandat 1603.366 kb on - strandat 1603.367 kb on + strandat 1603.370 kb on + strandat 1603.370 kb on + strandat 1603.372 kb on + strandat 1603.392 kb on + strandat 1603.412 kb on - strandat 1603.499 kb on - strand, within MMJJ_RS08710at 1603.500 kb on + strand, within MMJJ_RS08710at 1603.500 kb on + strand, within MMJJ_RS08710at 1603.500 kb on + strand, within MMJJ_RS08710at 1603.504 kb on - strand, within MMJJ_RS08710at 1603.504 kb on - strand, within MMJJ_RS08710at 1603.504 kb on - strand, within MMJJ_RS08710at 1603.505 kb on + strand, within MMJJ_RS08710at 1603.509 kb on + strand, within MMJJ_RS08710at 1603.548 kb on + strand, within MMJJ_RS08710at 1603.551 kb on + strand, within MMJJ_RS08710at 1603.555 kb on + strand, within MMJJ_RS08710at 1603.662 kb on - strand, within MMJJ_RS08710at 1603.663 kb on + strand, within MMJJ_RS08710at 1603.665 kb on - strand, within MMJJ_RS08710at 1603.681 kb on + strand, within MMJJ_RS08710at 1603.681 kb on + strand, within MMJJ_RS08710at 1603.681 kb on + strand, within MMJJ_RS08710at 1603.681 kb on + strand, within MMJJ_RS08710at 1603.682 kb on - strand, within MMJJ_RS08710at 1603.683 kb on + strand, within MMJJ_RS08710at 1603.683 kb on + strand, within MMJJ_RS08710at 1603.684 kb on - strand, within MMJJ_RS08710at 1603.684 kb on - strand, within MMJJ_RS08710at 1603.688 kb on + strand, within MMJJ_RS08710at 1603.688 kb on + strand, within MMJJ_RS08710at 1603.728 kb on + strand, within MMJJ_RS08710at 1603.766 kb on - strand, within MMJJ_RS08710at 1603.766 kb on - strand, within MMJJ_RS08710at 1603.770 kb on + strand, within MMJJ_RS08710at 1603.884 kb on - strand, within MMJJ_RS08710at 1603.899 kb on + strand, within MMJJ_RS08710at 1603.961 kb on - strand, within MMJJ_RS08710at 1603.985 kb on - strand, within MMJJ_RS08710at 1604.000 kb on + strand, within MMJJ_RS08710at 1604.004 kb on + strand, within MMJJ_RS08710at 1604.070 kb on - strand, within MMJJ_RS08710at 1604.142 kb on - strand, within MMJJ_RS08710at 1604.196 kb on - strandat 1604.196 kb on - strandat 1604.197 kb on + strandat 1604.197 kb on + strandat 1604.198 kb on - strandat 1604.199 kb on + strandat 1604.199 kb on + strandat 1604.199 kb on + strandat 1604.216 kb on + strandat 1604.246 kb on + strandat 1604.247 kb on - strandat 1604.251 kb on - strandat 1604.251 kb on - strandat 1604.251 kb on - strandat 1604.251 kb on - strandat 1604.251 kb on - strandat 1604.252 kb on + strandat 1604.252 kb on + strandat 1604.254 kb on - strandat 1604.254 kb on - strandat 1604.255 kb on + strandat 1604.259 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 & Casamino Acid FREE
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1,602,385 + -1.1
1,602,409 - +0.5
1,602,409 - -1.0
1,602,426 - +0.1
1,602,426 - -1.2
1,602,426 - -0.7
1,602,426 - +1.9
1,602,426 - -1.3
1,602,426 - +0.9
1,602,427 + +1.6
1,602,427 + -0.5
1,602,427 + +0.8
1,602,427 + +0.6
1,602,427 + +0.8
1,602,459 + +1.0
1,602,556 - MMJJ_RS08705 0.17 +1.4
1,602,560 - MMJJ_RS08705 0.17 +0.6
1,602,560 - MMJJ_RS08705 0.17 +0.0
1,602,561 + MMJJ_RS08705 0.17 +1.5
1,602,564 + MMJJ_RS08705 0.17 +1.0
1,602,568 - MMJJ_RS08705 0.18 +0.9
1,602,569 + MMJJ_RS08705 0.18 +0.8
1,602,610 - MMJJ_RS08705 0.22 +0.3
1,602,613 - MMJJ_RS08705 0.23 +2.0
1,602,613 - MMJJ_RS08705 0.23 +1.2
1,602,648 - MMJJ_RS08705 0.27 +0.8
1,602,648 - MMJJ_RS08705 0.27 +0.5
1,602,649 + MMJJ_RS08705 0.27 -1.7
1,602,650 - MMJJ_RS08705 0.27 +1.2
1,602,650 - MMJJ_RS08705 0.27 -0.9
1,602,650 - MMJJ_RS08705 0.27 +2.3
1,602,651 + MMJJ_RS08705 0.27 -2.1
1,602,651 + MMJJ_RS08705 0.27 +1.2
1,602,701 + MMJJ_RS08705 0.32 -1.7
1,602,834 - MMJJ_RS08705 0.47 +0.9
1,602,834 - MMJJ_RS08705 0.47 +0.3
1,602,834 - MMJJ_RS08705 0.47 +0.2
1,602,835 + MMJJ_RS08705 0.47 +0.8
1,602,835 + MMJJ_RS08705 0.47 +0.8
1,602,835 + MMJJ_RS08705 0.47 +0.9
1,602,914 + MMJJ_RS08705 0.56 -1.2
1,602,931 - MMJJ_RS08705 0.57 +0.4
1,603,016 + MMJJ_RS08705 0.67 +2.1
1,603,090 - MMJJ_RS08705 0.75 +0.7
1,603,103 + MMJJ_RS08705 0.76 +1.2
1,603,157 + MMJJ_RS08705 0.82 -0.7
1,603,160 - MMJJ_RS08705 0.83 -1.0
1,603,210 - MMJJ_RS08705 0.88 +0.5
1,603,210 - MMJJ_RS08705 0.88 +1.7
1,603,240 - +1.1
1,603,279 - -1.3
1,603,280 + +0.7
1,603,280 + -0.6
1,603,280 + +0.1
1,603,301 + -0.7
1,603,301 + -1.0
1,603,305 - +0.9
1,603,306 + -1.1
1,603,312 - -1.3
1,603,330 - -0.1
1,603,345 - +0.9
1,603,345 - -0.5
1,603,363 - -1.3
1,603,363 - -0.7
1,603,364 + -0.2
1,603,364 + +2.0
1,603,366 - +1.2
1,603,366 - +1.3
1,603,367 + -2.0
1,603,370 + +1.0
1,603,370 + +1.5
1,603,372 + -1.1
1,603,392 + +1.3
1,603,412 - -1.3
1,603,499 - MMJJ_RS08710 0.12 -0.9
1,603,500 + MMJJ_RS08710 0.13 +1.5
1,603,500 + MMJJ_RS08710 0.13 -0.9
1,603,500 + MMJJ_RS08710 0.13 -1.5
1,603,504 - MMJJ_RS08710 0.13 -0.7
1,603,504 - MMJJ_RS08710 0.13 -3.2
1,603,504 - MMJJ_RS08710 0.13 +0.0
1,603,505 + MMJJ_RS08710 0.13 -0.9
1,603,509 + MMJJ_RS08710 0.14 -0.3
1,603,548 + MMJJ_RS08710 0.18 -0.9
1,603,551 + MMJJ_RS08710 0.19 -1.5
1,603,555 + MMJJ_RS08710 0.19 -0.8
1,603,662 - MMJJ_RS08710 0.32 -1.4
1,603,663 + MMJJ_RS08710 0.32 -0.9
1,603,665 - MMJJ_RS08710 0.32 -2.3
1,603,681 + MMJJ_RS08710 0.34 -0.7
1,603,681 + MMJJ_RS08710 0.34 +0.1
1,603,681 + MMJJ_RS08710 0.34 +1.0
1,603,681 + MMJJ_RS08710 0.34 +1.4
1,603,682 - MMJJ_RS08710 0.34 +0.2
1,603,683 + MMJJ_RS08710 0.34 -0.5
1,603,683 + MMJJ_RS08710 0.34 +1.5
1,603,684 - MMJJ_RS08710 0.34 +0.2
1,603,684 - MMJJ_RS08710 0.34 -0.8
1,603,688 + MMJJ_RS08710 0.35 -0.9
1,603,688 + MMJJ_RS08710 0.35 +1.0
1,603,728 + MMJJ_RS08710 0.39 -0.3
1,603,766 - MMJJ_RS08710 0.44 +3.0
1,603,766 - MMJJ_RS08710 0.44 -0.3
1,603,770 + MMJJ_RS08710 0.44 -0.5
1,603,884 - MMJJ_RS08710 0.58 -0.7
1,603,899 + MMJJ_RS08710 0.60 -0.2
1,603,961 - MMJJ_RS08710 0.67 +0.0
1,603,985 - MMJJ_RS08710 0.70 +0.2
1,604,000 + MMJJ_RS08710 0.71 -1.1
1,604,004 + MMJJ_RS08710 0.72 -0.1
1,604,070 - MMJJ_RS08710 0.80 -2.2
1,604,142 - MMJJ_RS08710 0.88 -1.3
1,604,196 - +0.5
1,604,196 - -0.5
1,604,197 + -0.6
1,604,197 + -0.1
1,604,198 - -0.1
1,604,199 + +0.0
1,604,199 + +2.0
1,604,199 + -0.3
1,604,216 + -0.3
1,604,246 + -1.4
1,604,247 - -1.9
1,604,251 - -0.2
1,604,251 - +0.2
1,604,251 - -1.1
1,604,251 - -0.3
1,604,251 - -1.2
1,604,252 + -1.3
1,604,252 + +0.5
1,604,254 - -1.3
1,604,254 - -1.3
1,604,255 + +0.5
1,604,259 - -1.1

Or see this region's nucleotide sequence